Device for selectively available phase-contrast and relief observation in microscopes
First Claim
1. In a microscope, illumination-optical means establishing an illumination-ray path, an objective and observation-optical means establishing an observation-ray path, a first pupil plane in the illumination-ray path conjugate to a second pupil plane in the observation-ray path, a phase-contrast layer positioned in said second pupil plane, said phase-contrast layer having the configuration of at least one circular annulus concentric with the axis of the observation-ray path at said second pupil plane, the outer diameter of said annulus being of effectively pupil-entrance size at said second plane, and selectively operable means including at least two diaphragms of different configuration at said first pupil plane, one of said two diaphragms having the shape of a circular annulus, and the other of said two diaphragms being a light transmitting circular-ring segment.
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Abstract
The invention contemplates a microscope construction, applicable both to reflected-light and to transmitted-light varieties, whereby an object under examination can be selectively observed for phase-contrast and for relief effect (so-called "contrast modulation"). A first diaphragm having the effective shape of a circular ring is selectively positionable concentric with the observation-ray path and at a first location conjugate to the image plane of the microscope objective; and at least two diaphragms are selectively positionable on the illumination-ray path at a second location conjugate to the image plane of the microscope objective. One of these latter two diaphragms is in the effective circular-ring shape of the observation-path diaphragm, and the other of these latter two diaphragms is a light-transmitting circular-ring segment.
66 Citations
13 Claims
- 1. In a microscope, illumination-optical means establishing an illumination-ray path, an objective and observation-optical means establishing an observation-ray path, a first pupil plane in the illumination-ray path conjugate to a second pupil plane in the observation-ray path, a phase-contrast layer positioned in said second pupil plane, said phase-contrast layer having the configuration of at least one circular annulus concentric with the axis of the observation-ray path at said second pupil plane, the outer diameter of said annulus being of effectively pupil-entrance size at said second plane, and selectively operable means including at least two diaphragms of different configuration at said first pupil plane, one of said two diaphragms having the shape of a circular annulus, and the other of said two diaphragms being a light transmitting circular-ring segment.
Specification