Fermentable sugar from the hydrolysis of starch derived from dry milled cereal grains

• US 4,407,955 A
• Filed: 11/12/1981
• Issued: 10/04/1983
• Est. Priority Date: 11/12/1981
• Status: Expired due to Fees

• Current Assignee: National Distillers and Chemical Corporation
• Original Assignee: National Distillers and Chemical Corporation
• Inventors: Miller, Franklyn D., Muller, Werner C.
• Primary Examiner(s): Smith, William F.
• Application Number:
  US06/320,278
• Time in Patent Office: 691 Days
• Field of Search: 127/37, 127/38, 127/39, 127/40, 435/161, 203/19, 203/DIG. 13
• US Class Current: 435/161
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C13K 1/06 obtained by saccharification of starch or raw materials containing starch
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 203/14 Ejector-eductor

Abstract

Starch derived from a dry milled cereal grain such as corn or milo is hydrolyzed to provide a sterile aqueous fermentable sugar solution which is especially adapted for fermentative conversion to ethanol with minimum thermal expenditure. Following a preliminary acid-catalyzed hydrolysis of the starch to provide a sterile hydrolysate slurry, the slurry is further hydrolyzed in the presence of added aqueous non-fermentable carbohydrate to reequilibrate the hydrolysis reaction in favor of increased production of fermentable sugar, primarily glucose. Substantially all of the water insoluble protein and oil components, and a portion of the water soluble components, e.g., sugars, proteins and vitamins, are separately recovered from the sterile hydrolysate either before or after the further hydrolysis step with the water solubles being recycled to the system to effect reequilibration of a further quantity of hydrolysate.

Claims

1. A process for converting the starch fraction derived from whole dry milled cereal grain to a sterile aqueous solution of fermentable sugar, said starch containing water insoluble protein and oil, which comprises:

(a) hydrolyzing in a preliminary hydrolysis step an aqueous slurry of the starch in the presence of an acid hydrolysis catalyst at an elevated pressure and temperature to provide a starch hydrolysate slurry containing water insoluble protein and oil and water soluble components;

(b) separating water insoluble protein and oil with or without water soluble components from the hydrolysate resulting from step (a); and

,

(c) hydrolyzing in a further hydrolysis step the aqueous sterile starch hydrolysate slurry resulting from step (a) in the presence of added aqueous water soluble non-fermentable carbohydrate to provide a sterile aqueous solution of fermentable sugar.

3. The process of claim 1 or 2 wherein step (c) is carried out at an elevated pressure and temperature which is less than the pressure and temperature of step (a).

4. The process of claim 1 or 2 wherein the cereal grain feed for hydrolyzing step (a) is corn or milo.

5. The process of claim 1 or 2 wherein the cereal grain feed for hydrolyzing step (a) is dehulled corn or milo.

6. The process of claim 1 or 2 wherein the cereal grain feed for hydrolyzing step (a) is degerminated corn or milo.

7. The process of claim 1 or 2 wherein the sterile aqueous solution of fermentable sugar is subjected to fermentation to provide dilute aqueous ethanol.

8. The process of claim 7 wherein the concentrated ethanol is substantially dehydrated to provide anhydrous ethanol.

9. The process of claim 1 or 2 further comprising the steps of:

(d) diluting the water insoluble protein and oil resulting from step (c) with water;

(e) separating water from the water insoluble protein and oil resulting from step (d); and

,

(f) recycling the water recovered in step (e) as a source of water soluble carbohydrate for use in hydrolysis step (c) and/or for preparing the starch slurry for hydrolysis step (a).

10. The process of claim 9 wherein the dilution water in step (b) is stillage from an ethanol concentration facility.

11. The process of claim 1 or 2 wherein the aqueous slurry of starch is prepared with stillage from an ethanol concentration facility.

12. The process of claim 1 or 2 wherein stillage from an ethanol concentration facility is used as a source of water soluble non-fermentable carbohydrate for use in hydrolysis step (c).

13. The process of claim 9 wherein the stillage is contacted with an ion exchange resin prior to use in forming the starch slurry.

14. The process of claim 10 wherein the stillage is contacted with an ion exchange resin prior to use in hydrolysis step (c).

2. A process for converting the starch fraction derived from whole dry milled cereal grain to a sterile aqueous solution of fermentable sugar, said starch containing water insoluble protein and oil, which comprises:

(a) hydrolyzing in a preliminary hydrolysis step an aqueous slurry of the starch in the presence of an acid hydrolysis catalyst at an elevated pressure and temperature to provide a starch hydrolysate slurry containing water insoluble protein and oil and water soluble components;

(b) hydrolyzing in a further hydrolysis step the aqueous sterile starch hydrolysate slurry resulting from step (a) in the presence of added aqueous water soluble non-fermentable carbohydrate to provide a sterile aqueous solution of fermentable sugar; and

(c) separating water insoluble protein and oil with or without water soluble components from the hydrolysate resulting from step (b).