Saccharification method

• US 4,409,329 A
• Filed: 07/15/1982
• Issued: 10/11/1983
• Est. Priority Date: 03/23/1981
• Status: Expired due to Fees

• Current Assignee: Chevron Research Company (Chevron Corp.)
• Original Assignee: Gulf Research & Development Company
• Inventors: Silver, Richard S.
• Primary Examiner(s): Yoncoskie, Robert A.
• Application Number:
  US06/398,618
• Time in Patent Office: 453 Days
• Field of Search: 435/93, 435/99, 435/105, 435/161, 435/162, 435/163, 435/165, 435/305, 435/307, 435/813
• US Class Current: 435/105
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Enzymic conversion of cellulose under mechanical shear rate of from 50,000 to 200,000 feet/minute/foot as imparted to the cellulose by a series of rotors and stators mounted in the reaction vessel improves yield of simple sugars.

Claims

1. A method for the hydrolysis of cellulose to simple sugars which comprises:

contacting in a reaction zone an aqueous slurry comprising from three to 20 weight percent of a solid cellulose containing charge stock with a cellulase enzyme complex wherein the concentration of said enzyme complex is greater than 0.1 units per milliliter of said slurry and wherein said contacting occurs in the presence of a mechanically produced shear rate of from 50,000 to 200,000 feet/minute/foot substantially throughout said reaction zone and wherein the cellulose content of said solid cellulose-containing charge stock is from 30 to 80 weight percent of said charge stock.

2. A method in accordance with claim 1 wherein said shear rate is generated by a series of spaced apart rotors and stators mounted in said reaction zone containing the enzyme complex in said aqueous slurry.

3. A method in accordance with claim 2 wherein said solid cellulose-containing charge stock has a mean particle size from 0.01 to 1 inch in diameter.

4. A method in accordance with claim 2 wherein the rotors and stators comprise less than 50 percent of the reactor volume.

5. A method in accordance with claim 2 wherein the spacing between the rotors and stators is from 0.1 to 50 mm.

6. A method in accordance with claim 5 wherein said solid cellulose-containing charge stock has a mean particle size such that substantially no particles are larger than the spacing between rotor and stator blades.

7. A process in accordance with claim 1 wherein said process is operated in a conjoint presence of a fermentation microorganism for the simultaneous hydrolysis and fermentation of cellulose to ethanol.

8. A process in accordance with claim 6 wherein said fermentation microorganism is selected from the group consisting of yeasts such as Saccharomyces cerevisiae or Candida brassicae or bacteria such as Zymomonas mobilis or molds such as Rhizopus javanicus.

9. A process in accordance with claim 7 wherein the concentration of said enzyme complex is from 0.1 to 5 units per milliliter of said slurry.

10. A process in accordance with claim 8 wherein the concentration of said fermentation microorganism is such that the concentration of produced sugar is at all times substantially zero.