Apparatus for use in qualitative determination of immunological reactions

• US 4,411,518 A
• Filed: 11/26/1979
• Issued: 10/25/1983
• Est. Priority Date: 09/23/1977
• Status: Expired due to Term

• Current Assignee: Gamma Biologicals, Inc. (Werfen SA)
• Original Assignee: Gamma Biologicals, Inc. (Werfen SA)
• Inventors: Acker, Jesse L., Meserol, Peter M.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US06/097,195
• Time in Patent Office: 1,429 Days
• Field of Search: 356/244, 356/246, 356/39, 356/440, 350/95, 250/576, 73/426, 73/64.1, 422/104, 422/102, 422/73, 424/12
• US Class Current: 356/39
• CPC Class Codes: G01N 2035/00099 Characterised by type of test elements
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/5911 of the scanning type scanning per se G02B
• CPC Class Codes: G01N 21/82 producing a precipitate or turbidity
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

An apparatus is provided for use in qualitative and quantitative determinations of agglutination in immunoreactive systems. The apparatus comprises a carriage assembly having a plurality of spaced, axially-disposed compartments for receiving image cells which are uniquely designed for holding the biological fluid test specimen and the reagent therefor. Means are provided for partially lifting each image cell successively out of its compartment and into an optical path. A monochromatic light source directs beams of radiant energy through the reaction zone in each image cell and the light beams transmitted through the reaction zone are focused, by means of an imaging lens, on the surface of an image sensor such as a "Charge-Coupled Device". The dark areas formed on the image sensor are measured electronically and also displayed on a display cathode ray tube.

Claims

1. An apparatus for use in connection with immunological reactions, said apparatus comprising:

(a) a carriage assembly defined by front and rear walls and by side walls, said carriage assembly comprising a plurality of spaced, axially-disposed compartments between said side walls,

(b) an image cell in each of said compartments, each image cell comprising two opposed, parallel, planar surfaces wherein each of said surfaces includes a generally circular groove so that when said surfaces are bonded together, said circular grooves define a reaction cell, and aperture in one of said grooves for introducing biological fluid and reagent into said reaction cell,

(c) means for engaging with and biasing each of said image cells partially out of its respective compartment and into an optical path,

(d) a light source for directing beams of light through said reaction cell,

(e) an image sensor comprising a photosensitive surface consisting of photosensor elements,

(f) an imaging lens for focusing the light beams transmitted through said reaction cell onto said image sensor on which dark images corresponding to agglutinated particles in the reaction cells are formed, and

(g) means for determining the total dark imaged area on the surface of said image sensor.

2. An apparatus as in claim 1 wherein said carriage assembly includes at least two compartments.

3. An apparatus as in claim 1 wherein both said surfaces of said image cells are made of a transparent material.

4. An apparatus as in claim 2 wherein both said surfaces of said image cells are made of a transparent material.

5. An apparatus as in claim 3 wherein one of said surfaces is made of a transparent material and the other surface is made of a translucent material.

6. An apparatus as in claim 4 wherein one of said surfaces is made of a transparent material and the other surface is made of a translucent material.

7. An apparatus as in claim 1 wherein said means for engaging with and biasing each said image cell is a caliper means.

8. An apparatus as in claim 2 wherein said means for engaging with and biasing each said image cell is a caliper means.

9. An apparatus as in claim 3 wherein said means for engaging with and biasing each said image cell is a caliper means.

10. An apparatus as in claim 4 wherein said means for engaging with and biasing each said image cell is a caliper means.

11. An apparatus as in claim 5 wherein said means for engaging with and biasing each said image cell is a caliper means.

12. An apparatus as in claim 6 wherein said means for engaging with and biasing each said image cell is a caliper means.

13. An apparatus as in claim 1 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

14. An apparatus as in claim 2 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

15. An apparatus as in claim 3 wherein said means for determining the total dark imaged area of the surface of said image sensor is an electronic means.

16. An apparatus as in claim 4 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

17. An apparatus as in claim 5 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

18. An apparatus as in claim 6 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

19. An apparatus as in claim 7 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

20. An apparatus as in claim 8 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

21. An apparatus as in claim 9 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

22. An apparatus as in claim 10 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

23. An apparatus as in claim 11 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

24. An apparatus as in claim 12 wherein said means for determining the total dark imaged area on the surface of said image sensor is an electronic means.

25. An image cell for fluid reactants, said image cell comprising a pair of opposed, parallel planar surfaces bonded to each other, a generally circular indentation in each of said opposed surfaces so as to define a reaction cell, and an aperture in one of said indentations for introducing the fluid reactants into said reaction cell, wherein said indentations are sufficiently recessed so as to permit the fluid reactants to freely move within said cell while said cell is rotated and wherein said reactants remain confined without issuing from said cell during such rotation.

26. An image cell as in claim 25 wherein one of said surfaces are made from a transparent material.

27. An image cell as in claim 25 wherein one of said surfaces is made from a transparent material and the other surface is made of a translucent material.

28. An image cell as in claim 25 further including a generally cylindrical member attached on the inner face of one of said circular indentations for focusing an optical system on the mid-plane in the reaction cell.

29. An image cell as in claim 26 further including a generally cylindrical member attached on the inner face of one of said circular indentations for focusing an optical system in the mid-plane in the reaction cell.

30. An image cell as in claim 27 further including a generally cylindrical member attached on the inner face of one of said circular indentations for focusing an optical system on the mid-plane in the reaction cell.