Experiment-machine

• US 4,412,288 A
• Filed: 04/01/1980
• Issued: 10/25/1983
• Est. Priority Date: 04/01/1980
• Status: Expired due to Term

• Current Assignee: Michael Herman
• Original Assignee: Michael Herman
• Inventors: Herman, Michael
• Primary Examiner(s): ATKINSON, CHARLES
• Application Number:
  US06/136,326
• Time in Patent Office: 1,302 Days
• Field of Search: 364/413, 364/414, 364/415, 364/200, 364/900, 128/630, 128/653, 128/634, 250/445 R, 250/445 T
• US Class Current: 600/342
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: Y10S 128/92 Computer assisted medical diagnostics

Abstract

A method, apparatus and procedure whereby a probe, which in the preferred embodiment is a fiber optic catheter, is used in vivo to acquire intra-cellular data from a host. Under computer control, the system detects the specific nature of the host subject'"'"'s cell kinetic processes. Cells are assumed to always acquire information about their environs and this information is detected and recognized as constituting friendly or unfriendly environments. Using physical analogies, the computer tracks pairing and unpairing of nucleotide sequences as biological radar. A reference analog is used to vary the view of the data-base so that selected portions or subsets of the available analogies lend themselves to sensory presentation from the computer to the user. Provision is made for interactive human communication with the host'"'"'s metabolism while microscopic resolution and forecasting are obtained. The method and procedure is analog driven and may be embodied in a variety of hardware and software mixes.

Claims

1. A biological diagnostic and testing system comprising:

probe means for examining intra-cellular activity within an individual cell in animal tissue in vivo and selectively providing a physical indication signal representing that activity;

control means for selectively actuating said probe means to provide said physical indication signal; and

display means for providing a visual display in real time of the intra-cellular activity represented by said physical indication signal.

2. The system according to claim 1:

wherein said control means includes means for selectively delivering an interrogation signal, in the form of an energy burst, to said probe means; and

wherein said probe means comprises transmit means for directing interrogation signal energy bursts delivered thereto toward a target area within said individual cell and receiver means for receiving, as said physical indication signal, a reflection of said energy burst from said target area.

3. The system according to claim 2 wherein said control means further comprises:

manipulator means for automatically maintaining said probe means directed toward said target area in spite of movement of said animal tissue.

4. The system according to claim 2 wherein said control means further comprises:

means for detecting changes in successive physical indication signals;

means for determining if the detected changes are the result of relative movement between said probe means and said animal tissue; and

manipulator means for re-positioning said probe means, in response to a determination of relative movement between said probe means and animal tissue, to maintain said probe means directed toward said target area.

5. The system according to claims 2 or 4:

wherein said probe means comprises at least first and second separate probe elements, each directed to target areas within respective individual cells in said animal tissue, each including transmit means responsive to energy bursts delivered thereto for directing such bursts toward the target areas of that probe element, and each including receiver means for receiving reflections of said energy bursts; and

wherein said control means includes multiplexer means for delivering energy bursts to said first probe element which differ in a physical parameter from energy bursts delivered to said second probe element.

6. The system according to claim 5 wherein said energy bursts are light bursts and wherein said probe elements are fiber optic probes.

7. The system according to claim 6 wherein said physical parameter which differentiates energy bursts delivered to said first and second probe elements is time.

8. The system according to claims 2 or 4 wherein said probe means comprises at least first and second separate probe elements of different types, each probe element being directed to a respective target area within different individual cells in said animal tissue, each probe element including transmit means responsive to energy bursts delivered thereto for directing the delivered bursts toward the target area if that probe element, and each probe element includes receiver means for receiving reflections of said energy bursts, and wherein said energy burst delivered to said first probe element is a different energy form than the energy burst delivered to said second probe element.

9. The system according to claim 8 wherein said first probe element is a fiber optic probe and the energy burst delivered thereto is light energy.

10. The system according to claim 4 wherein said control means further comprises means for selectively expanding and decreasing the field of view of said target area.

11. A method of biological diagnosis and testing comprising the steps of:

examining intra-cellular activity within an individual cell in animal tissue in vivo with a probe and selectively providing a physical indication signal representing that activity;

selectively actuating said probe to provide said physical indication signal; and

providing a visual display in real time of the intracellular activity represented by said physical indication signal.

12. The method according to claim 11:

wherein said step of actuating includes selectively delivering an interrogation signal, in the form of an energy burst, to said probe; and

wherein said step of examining comprises transmitting interrogation signal energy bursts, delivered to said probe, toward a target area within said individual cell, and receiving, as said physical indication signal, a reflection of said energy burst from said target area.

13. The method according to claim 12 further comprising the step of:

automatically maintaining said probe directed toward said target area in spite of movement of said animal tissue.

14. The method according to claim 12 further comprising the steps of:

detecting changes in successive physical indication signals;

determining if the detected changes are the result of relative movement between said probe and said animal tissue; and

re-positioning said probe, in response to a determination of relative movement between said probe means and animal tissue, to maintain said probe directed toward said target area.

15. The method according to claims 12 or 14 wherein said probe comprises at least first and second separate probe elements, and further comprising the step of:

delivering energy bursts to said first probe element which differ in a physical parameter from energy bursts delivered to said second probe element.

16. The method according to claim 15 wherein said energy bursts are light bursts.

17. The method according to claim 16 wherein said physical parameter which differentiates energy bursts delivered to said first and second probe elements is time.

18. The method according to claims 12 or 14 wherein said probe means comprises at least first and second separate probe elements of different types, and wherein said energy burst delivered to said first probe element is a different energy form than the energy burst delivered to said second probe element.

19. The method according to claim 18 wherein the energy burst delivered thereto is light energy.

20. The method according to to claim 14 further comprising the step of selectively expanding and decreasing the field of view of said target areas.

21. A biological diagnostic and testing system for monitoring intra-cellular activity within a common host, said system comprising:

a plurality of individually actuable probe elements for examining intra-cellular activity within respective individual cells in said host, each probe element being responsive to reception of an interrogation signal for sensing a physical parameter within a localized area in its respective cell and providing a measurement signal representative of the state of that parameter;

control means for selectively actuating each of said probe elements with respective interrogation signals; and

processor means for receiving and individually monitoring the measurement signals provided by said probe elements.

22. The system according to claim 21 wherein said control means comprises multiplexer means for applying interrogation signals to said probe elements in a prescribed time sequence.

23. A method of biological diagnosis comprising the steps of:

(a) selectively examining intra-cellular activity within an individual cell in animal tissue in vivo and providing an indication signal representing that activity;

(b) comparing the provided indication signal with stored medical history data; and

(c) determining, from the comparison in step (b) whether or not a predetermined disease condition is indicated by the examined intra-cellular activity.

24. The method according to claim 23 wherein step (a) includes:

sequentially examining different areas within a cell and providing respective indication signal corresponding to the intra-cellular activity in each area.

25. The method according to claims 23 or 24 wherein step (a) includes:

examining intra-cellular activity within plural individual cells of said host with respective individual probe members and providing respective indication signals from each probe member.

26. The method according to claim 25 wherein said predetermined disease condition is a carcinogenic condition.

27. The method according to claims 23 or 24 wherein said predetermined disease condition is a carcinogenic condition.

28. The method according to claim 23 or 24 wherein step (a) includes:

selectively actuating a probe to provide said physical indication signal.

29. The method according to claim 28 wherein said step of actuating includes selectively delivering an interrogation signal, in the form of an energy burst, to said probe;

and

wherein said step of examining comprises transmitting interrogation signal energy bursts, delivered to said probe, toward a target area within said individual cell, and receiving, as said physical indication signal, a reflection of said energy burst from said target area.

30. The method according to claim 29 further comprising the step of:

automatically maintaining said probe directed toward said target area in spite of movement of said animal tissue.

31. The method according to claim 29 further comprising the steps of:

detecting changes in successive physical indication signals;

determining if the detected changes are the result of relative movement between said probe and said animal tissue; and

re-positioning said probe, in response to a determination of relative movement between said probe means and animal tissue, to maintain said probe directed toward said target area.

32. The method according to claim 31 wherein said probe comprises at least first and second separate probe elements, and further comprising the step of:

delivering energy bursts to said first probe element which differ in a physical parameter from energy bursts delivered to said second probe element.

33. The method according to claim 29 wherein said probe comprises at least first and second separate probe elements, and further comprising the step of:

delivering energy bursts to said first probe element which differ in a physical parameter from energy bursts delivered to said second probe element.

34. The method according to claim 33 wherein said energy bursts are light bursts.

35. The method according to claim 34 wherein said physical parameter which differentiates energy bursts delivered to said first and second probe elements is time.

36. The method according to claim 31 wherein said probe means comprises at least first and second separate probe elements of different types, and wherein said energy burst delivered to said first probe element is a different energy form than the energy burst delivered to said second probe element.

37. The method according to claim 29 wherein said probe means comprises at least first and second separate probe elements of different types, and wherein said energy burst delivered to said first probe element is a different energy form than the energy burst delivered to said second probe element.

38. The method according to claim 29 further comprising the step of selectively expanding and decreasing the field of view of said target areas.