Tissue adhesive

• US 4,414,976 A
• Filed: 09/13/1982
• Issued: 11/15/1983
• Est. Priority Date: 02/15/1979
• Status: Expired due to Term

• Current Assignee: Immuno AG Fur Chemisch-Medizinische Produkte
• Original Assignee: Immuno AG Fur Chemisch-Medizinische Produkte
• Inventors: Seelich, Thomas, Linnau, Yendra, Schwarz, Otto, Loblich, Franz
• Primary Examiner(s): Morris, Theodore
• Application Number:
  US06/417,538
• Time in Patent Office: 428 Days
• Field of Search: 424/101, 424/177, 260/112 B, 106/161, 128/334 R
• US Class Current: 606/214
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/16 Blood plasma; Blood serum umbilical cord blood A61K35/51
• CPC Class Codes: A61K 38/005 Enzyme inhibitors protease inhibitors A61K38/55
• CPC Class Codes: A61L 24/106 Fibrin; Fibrinogen
• CPC Class Codes: Y10S 530/83 Plasma; serum

Abstract

A tissue adhesive on the basis of human or animal protein contains factor XIII and at least 33% by weight of fibrinogen, has a ratio of factor XIII to fibrinogen, expressed in units of factor XIII per gram of fibrinogen, of at least 80, contains fibrinogen and albumin in the total protein at a ratio of 33 to 90:5 to 40, contains plasminogen-activator-inhibitor or plasmin inhibitor in an amount of 250 to 25,000 KIU per g of fibrinogen and has been lyophilized.

Claims

1. A method of seamlessly connecting tissue or organ parts, for sealing wounds, stopping bleeding and stimulating wound healing in mammals which comprises reconstituting lyophilized tissue adhesive of mammalian protein origin which comprises fibrinogen, albumin, factor XIII, cold-insoluble globulin and plasminogen-activator inhibitor or plasmin inhibitor wherein the fibrinogen is present in at least 33% by weight, in the lyophilized state, the ratio of factor XIII to fibrinogen, expressed in units of factor XIII per gram of fibrinogen is at least 80, and fibrinogen and albumin are present in a ratio of 33 to 90:

5 to 40 and applying said reconstituted tissue adhesive containing at least 70 mg of fibrinogen per ml to the organ tissue or wound of a mammal in an amount sufficient to function as an adhesive.

2. A method as set forth in claim 1, wherein the tissue adhesive and a mixture of thrombin and calcium chloride are applied onto the tissue.

3. A method as set forth in claim 1, wherein, prior to applying the tissue adhesive onto the tissue to be connected, a mixture of thrombin and calcium chloride is added to the adhesive.