Support for electrophoresis and method of producing same

• US 4,415,428 A
• Filed: 01/27/1982
• Issued: 11/15/1983
• Est. Priority Date: 01/27/1982
• Status: Expired due to Term

• Current Assignee: FMC Corporation
• Original Assignee: FMC Corporation
• Inventors: Nochumson, Samuel, Witt, Henry J.
• Primary Examiner(s): Douglas, Winston A.
• Assistant Examiner(s): Boggs, Jr., B. J.
• Application Number:
  US06/342,993
• Time in Patent Office: 657 Days
• Field of Search: 204/180 G, 204/180 S, 204/299 R, 204/301
• US Class Current: 204/606
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture

Abstract

An electrophoretic support comprising a base plate having on at least one side thereof, a layer of an adherent resin containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide. The support provides excellent adhesion for polyacrylamide gels and other electrophoretic media such as agarose.

Claims

1. A support for an electrophoretic medium comprising a base plate of an organoplastic sheet film and an adherent resin coating applied to at least one side of said base plate, said resin coating containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide.

2. The support according to claim 1 wherein the organo plastic sheet film is selected from the class consisting essentially of polystyrene, polypropylene, styrene-acrylonitrile copolymer, polycarbonate, cellulose acetate propionate, cellulose acetate, butyrate, nitrile-acrylonitrile-styrene terpolymer, polyacrylate, polymethacrylate, polyterephthalate and acrylonitrile-butadiene-styrene copolymer.

3. The support of claim 1 wherein the ethylenically unsaturated groups in the resin consist of ethylenically unsaturated hydrocarbon radicals of 2 to 12 carbon atoms connected to said resin through functional linkages selected from the class consisting of --O--, --S--, --N--, --O(CO)--, S(CO)-- and --(CO)N-- linkages.

4. The support according to claim 1 wherein the resin coating is in the form of a separately applied film-forming adherent coating.

5. The support according to claim 4 wherein the coated side of the base plate and the resin coating are hydrophilic.

6. The support according to claim 5 wherein the hydrophilic surface on the coated side of the organoplastic sheet film is formed by contacting it with an electric discharge.

7. The support according to claim 3 wherein the resin is a polysaccharide.

8. The support according to claim 7 wherein the polysaccharide is agarose.

11. An electrophoretic element, for use in electrophoretic separations, comprising the support of claim 1 coated with electrophoretic medium.

12. The electrophoretic element of claim 11 wherein the electrophoretic medium is selected from the class consisting of polyacrylamide and agarose gel films.

9. An electrophoretic support comprising a base plate of an organoplastic film sheet one side of which has been rendered hydrophilic by contact with a plasma chamber and having applied to said hydrophilic side a coating of allylglycidylagarose.

10. The support according to claim 9 wherein the organoplastic film is polyester film.

13. An electrophoretic element, comprising the support of claim 9 coated with an electrophoretic medium.

14. The electrophoretic element of claim 13 wherein the electrophoretic medium is selected from the class consisting of polyacrylamide and agarose gel films.

15. The electrophoretic element of claim 14 wherein the electrophoretic medium is a polyacrylamide gel film.

16. The electrophoretic element of claim 14 wherein the electrophoretic medium is an agarose gel film.