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Multilayer enzyme electrode membrane

  • US 4,418,148 A
  • Filed: 11/05/1981
  • Issued: 11/29/1983
  • Est. Priority Date: 11/05/1981
  • Status: Expired due to Fees
First Claim
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1. A method of making a contiguous multilayer membrane of about 40 to about 100 microns in overall thickness suitable for use with an electrochemical sensor in the measurement of an unknown which comprises:

  • providing as a first layer, a polymer dissolved in an inert organic solvent and casting said polymer in solution onto an inert support surface which is unreactive with said polymer and does not form a bond to said polymer;

    permitting said solution to form a film and thereby obtaining a first relatively nonporous dense polymer layer of about 2 to about 5 microns in thickness;

    providing as a second layer, a composition comprising a polymer dissolved in an inert organic solvent, mixing said polymer dissolved in solvent with a nonsolvent for said polymer to obtain a dispersion and thereafter casting said dispersion onto said first layer and thereafter permitting said second layer to dry to form a porous polymer layer of about 2 to about 20 microns in thickness and less dense and more porous than the first layer;

    providing a third layer comprising an enzyme solution or suspension and depositing said third layer onto the exposed surface of the second layer;

    providing as a fourth layer, a composition comprising a polymer dissolved in an inert organic solvent, mixing said polymer dissolved in solvent with a nonsolvent for said polymer to obtain a dispersion and thereafter casing said dispersion onto the exposed surface of the third layer to provide a fourth porous polymer layer of about 2 to about 20 microns in thickness and less dense and more porous than the first layer; and

    thereby forming said contiguous multilayer membrane.

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