Filteration method for cell produced antiviral substances
First Claim
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1. A separation method for extracting Cell-Produced-Antiviral-Substances (CPAS) from a production broth containing the living cells which produce the CPAS consisting essentially of:
- subjecting said broth and said living cells to a non-dead-end membrane filtration wherein the broth and said living cells are perfused into a first filtration cell and caused to flow across an ultra-filtration membrane in said cell which membrane has a nominal molecular weight cut-off of from twice to about five times the maximum molecular weight of the CPAS,permitting a first filtrate to pass through said membrane consisting essentially of CPAS and some molecular species,retaining in a first retentate all remaining portions of said broth, and wherein a diafiltration process is afforded by a partially-closed cycle continuous or semicontinuous flow, in which said first filtrate removed from the broth is replaced with an equal volume of media, thus maintaining a constant suspension volume throughout the process, and said first retentate is cycled back to the broth.
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Abstract
This invention affords a filtration method and apparatus for extracting cell produced antiviral substances (CPAS) from a production broth using cross-flow membrane filtration. The broth is perfused into a first filtration cell and caused to cross-flow across an ultrafiltration membrane in the cell, permitting a first filtrate to pass through the membrane consisting essentially of CPAS and some molecular species and retaining in a first ex-filtrate all remaining portions of the broth.
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24 Claims
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1. A separation method for extracting Cell-Produced-Antiviral-Substances (CPAS) from a production broth containing the living cells which produce the CPAS consisting essentially of:
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subjecting said broth and said living cells to a non-dead-end membrane filtration wherein the broth and said living cells are perfused into a first filtration cell and caused to flow across an ultra-filtration membrane in said cell which membrane has a nominal molecular weight cut-off of from twice to about five times the maximum molecular weight of the CPAS, permitting a first filtrate to pass through said membrane consisting essentially of CPAS and some molecular species, retaining in a first retentate all remaining portions of said broth, and wherein a diafiltration process is afforded by a partially-closed cycle continuous or semicontinuous flow, in which said first filtrate removed from the broth is replaced with an equal volume of media, thus maintaining a constant suspension volume throughout the process, and said first retentate is cycled back to the broth. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A dual module separation apparatus for extracting purified Cell-Produced-Antiviral-Substances (CPAS) from a CPAS production broth containing living cells which produce said CPAS consisting essentially of:
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a source of production broth containing said living cells; first non-dead-end filtration cell means, conduit means flowingly connecting said source of production broth containing said living cells to said first non-dead-end filtration cells means for flowing said broth and living cells across a filtration membrane for separating CPAS and all smaller molecules in a filtrate and recycling a retentate back to said source of production broth containing said living cells, which membrane has a nominal molecular weight cut-off of from twice to about five times the maximum molecular weight of said CPAS; CPAS reservoir means for receiving and storing said CPAS; conduit means flowingly connecting said first non-dead-end filtration cell means to said CPAS reservoir means for conducting said filtrate to said CPAS reservoir means; second filtration cell means for separating molecules smaller than CPAS into a second filtrate and a second retentate containing CPAS, said second filtration cell means having a filtration membrane with a nominal molecular weight cut-off of about 75 percent or less of the minimum molecular weight of the CPAS; and conduit means flowingly connecting said second filtration cell means with said CPAS reservoir for conducting fluid from said CPAS reservoir to said second filtration cell means and for recycling said second retentate back to the CPAS reservoir. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification