Method for processing protein from nonbinding oilseed by ultrafiltration and solubilization

• US 4,420,425 A
• Filed: 08/02/1982
• Issued: 12/13/1983
• Est. Priority Date: 08/02/1982
• Status: Expired due to Term

• Current Assignee: Texas A&M University System
• Original Assignee: Texas A&M University System
• Inventors: Lawhon, James T.
• Primary Examiner(s): SCHAIN, HOWARD
• Application Number:
  US06/404,125
• Time in Patent Office: 498 Days
• Field of Search: 260/112 R, 260/123.5
• US Class Current: 530/378
• CPC Class Codes: A23J 1/14 from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds

Abstract

There is provided a method of producing proteins from nonbinding oilseeds such as soybeans and peanuts by solubilization and ultrafiltration. A filterable protein feed is produced by extraction from a mixture of water and nonbinding oilseeds such as soybeans or peanuts. The feed is then passed over an ultrafiltration membrane system with a molecular weight cutoff of about 70,000 and preferably of 100,000 daltons or more. While the feed is being passed over the ultrafiltration membrane, the concentration or dilution of the protein feed is properly controlled in order to insure separation into the permeate of a substantial portion of that fraction of the constituents which produce color and flavor in the ultimate product.

Claims

1. A method of processing nonbinding oilseeds comprising the steps of:

solubilizing at least a portion of the protein contained in the oilseed to produce a filterable protein feed stream; and

passing the protein feed stream through an ultrafiltration membrane system with a molecular weight cutoff of about 70,000 daltons to separate out proteins, while controlling the concentration of the feed to limit self filtering and produce a permeate and a retentate, the retentate having a substantially bland taste and colorless appearance when dried.

2. A method according to claim 1 where in the molecular weight cutoff of the ultrafiltration membrane system is at least about 100,000 daltons.

3. A method according to claims 1 or 2 wherein the filterable protein feed stream is produced by extracting oilseeds in an alkaline environment to produce a solubilized protein solution.

4. A method according to claims 1 or 2 wherein the filterable protein feed stream is produced by extracting oilseeds in an acidic environment to produce a dispersed protein slurry.

5. A process according to claims 1 or 2 wherein the oilseed is soybean and the soybean in the feed is initially in the form of defatted soybean.

6. The protein product produced from oilseeds by the process of claims 1 or 2.

7. The method of claims 1 or 2 further comprising the step of passing the permeate through a reverse osmosis membrane.

8. A process according to claim 7 wherein the permeate of the reverse osmosis membrane is essentially water.

9. A process according to claim 8 wherein the permeate of the reverse osmosis membrane is recirculated for solubilizing a portion of the oilseed.

10. A process according to claim 7 wherein at least a portion of the reverse osmosis membrane permeate is used to control the concentration of the feed stream prior to its passage through the ultrafiltration membrane system.

11. A method of processing nonbinding oilseed protein comprising the steps of:

extracting oilseeds in an alkaline environment to produce a solubilized protein solution;

removing at least a portion of any insoluble residue from the protein solution; and

passing the protein solution over an ultrafiltration membrane with a molecular weight cutoff of at least about 100,000 to separate out color causing and taste causing components and to produce permeate and a retentate, the retentate having a substantially bland taste and a colorless appearance when dry.

12. A process according to claim 11 wherein the concentration of the solution is controlled to limit self-filtering in the ultrafiltration membrane.

13. A process according to claim 12 wherein the step of controlling the concentration of the solution to limit self-filtering is accomplished by adding water to a supply zone essentially upon the initiation of ultrafiltration to essentially maintain the original volume of the solution in the supply zone.

14. A method according to claim 11 further comprising the step of pasteurizing the solubilized protein solution prior to passing the protein solution through the ultrafiltration membrane.

15. A process according to claim 11 wherein the oilseed is soybean and the soybean extracted is initially in the form of defatted soybean.

16. A process according to claim 11 wherein the solubilized protein solution initially comprises oilseed, water, and sodium hydroxide.

17. A process according to claim 11 wherein the solubilized protein solution initially comprises oilseed, water, and calcium hydroxide.

18. A process according to claims 11, 16 or 17 wherein the oilseeds are extracted in an alkaline solution and ratio of water to oilseed in the alkaline solution is at least 10 to 1.

19. A process according to claim 11 wherein the protein solution is fed from a supply region and further comprising the steps of concurrently recycling the retentate and passing water to the supply region, the amount of water and retentate being sufficient to maintain the volume of the solubilized protein solution at a substantially constant volume.

20. A process according to claim 19 wherein the volume of the solubilized protein solution is maintained at a constant level until a volume of permeate collected is at least 1.2 times as large as the constant volume.

21. The process according to claim 20 wherein the volume of permeate ultimately collected is between approximately 2 and 3 times as large as the constant volume.

22. A process according to claim 11 wherein the retentate is passed through at least one more ultrafiltration membrane.

23. The protein product produced from oilseed by the process of claim 11.

24. The protein product of claim 23 wherein the protein product is an isolate.

25. The protein product of claim 23 having a Gardner color difference meter reading of at least 80 when the product is dry.

26. A method of processing nonbinding oilseed protein having a substantially bland taste and colorless appearance comprising the steps of:

mixing oilseeds in water to produce a mixture;

regulating the pH of the mixture while controlling the temperature and concentration of the oilseeds in the mixture in order to dissolve oilseed protein and and produce a solubilized protein slurry comprising a protein solution;

separating out insolubilized solids from the protein slurry;

prefiltering the protein slurry to remove a sufficient amount of suspended matter in preparation for the ultrafiltration of the protein solution; and

passing the protein solution over at least one ultrafiltration membrane with a molecular weight cutoff of at least about 100,000 daltons to produce a permeate and a retentate while essentially maintaining the original volume of the protein solution in a supply zone as the solution is being ultrafiltered by adding water at a rate sufficient to compensate for the permeate being removed from the ultrafiltration membrane and any retentate not recycled to the supply zone.

27. A protein produced from oilseeds according to the process of claim 26.

28. A process according to claim 26 further comprising the step of passing the permeate from the ultrafiltration membrane through a reverse osmosis membrane to produce a reverse osmosis permeate and a reverse osmosis retentate, the reverse osmosis permeate consisting substantially of water.

29. A process according to claim 26 wherein the pH of the mixture is greater than 7.

30. A method of processing soy protein having a substantially bland taste and colorless appearance comprising the steps of:

mixing defatted soy flour in water to produce a mixture wherein the ratio of soy to water by weight is between about 40 to 1 and about 10 to 1 and the temperature of the mixture is between about 50 and about 60°

C.;

setting the pH of the mixture between about 7 and about 8 in order to dissolve soy protein and produce a solubilized protein slurry comprising a protein solution;

separating out insolubilized solids from the protein slurry;

prefiltering the protein slurry to remove a sufficient amount of suspended matter in preparation for the ultrafiltration of the protein solution;

passing the protein solution over at least one ultrafiltration membrane with a molecular weight cutoff of at least about 100,000 to produce a permeate and a retentate while substantially maintaining the original volume of the protein solution in a feed tank as the solution is being ultrafiltered by adding water to make up for permeate removed from the ultrafiltration membrane and any retentate not recycled to the feed tank while recycling at least a portion of the retentate to the feed tank and collecting the permeate;

allowing the volume of the protein solution in the feed tank to decrease once a volume of permeate equal to at least about 1.2 times the volume of the protein solution in the feed tank is collected; and

thereafter mixing any unrecycled retentate with the protein solution.

31. The protein product produced by the performance of the process defined in claim 30.

32. A process according to claim 30 further comprising the steps of:

allowing the volume of the protein solution in the feed tank to decrease until a volume of permeate equal to at least about 2.0 times the volume of the protein solution in the feed tank is collected; and

thereafter mixing any unrecycled retentate with the protein solution and spray drying the resultant mixture.

33. A method of producing nonbinding oilseed protein comprising the steps of:

extracting oilseeds in an acidic environment to produce a dispersed protein slurry; and

passing the protein slurry over an ultrafiltration membrane with a molecular weight cutoff of at least about 100,000 to separate out color causing and taste causing components and to produce a permeate and a retentate, the retentate having a substantially bland taste and a colorless appearance when dry.

34. A process according to claim 33 wherein the concentration of the slurry is controlled to limit self-filtering in the ultrafiltration membrane.

35. A process according to claim 33 wherein the step of controlling the concentration of the slurry to limit self-filtering is accomplished by adding water to a supply zone essentially upon the initiation of ultrafiltration to essentially maintain the original volume of the slurry in the supply zone.

36. A process according to claim 33 wherein the pH of the dispersed protein slurry is at least 4.5.

37. The protein product produced from oilseeds by the process of claim 33.

38. A method of producing a soy protein concentrate having a substantially bland taste and colorless appearance comprising the steps of:

mixing defatted soy flour in water to produce a mixture wherein the ratio of soy to water by weight is between about 40 to 1 and about 10 to 1 and the temperature of the mixture is between about 50 and about 60°

C.;

controlling the pH of the mixture to dissolve nonprotein constituents including sugars and salts and a portion of the protein and to produce a dispersed protein slurry comprising undissolved protein; and

passing the protein slurry over at least one ultrafiltration membrane with a molecular weight cutoff of at least about 100,000 to produce a permeate and a retentate while substantially maintaining the original volume of the protein slurry in a feed tank as the slurry is being ultrafiltered by adding water to make up for permeate removed from the ultrafiltration membrane and any retentate not recycled to the feed tank while recycling at least a portion of the retentate to the feed tank and collecting the permeate.

39. A process according to claim 38 further comprising the steps of:

allowing the volume of the protein slurry in the feed tank to decrease once a volume of permeate equal to at least about 1.2 times the volume of the protein slurry in the feed tank is collected; and

thereafter mixing any unrecycled retentate with the protein slurry.

40. A process according to claim 39 further comprising the steps of:

allowing the volume of the protein solution in the feed tank to decrease until a volume of permeate equal to at least about 2.0 times the volume of the protein solution in the feed tank is collected; and

thereafter mixing any unrecycled retentate with the protein solution and spray drying the resultant mixture.

41. The process of claims 38 or 39 wherein the pH of the flour-water mixture is set at approximately 4.5.

42. The process of claims 38 or 39 wherein the pH of the flour-water mixture is controlled by the addition of a suitable acid.

43. The process of claim 42 wherein the acid is hydrochloric acid.

44. The protein product produced by the performance of the process defined in claim 38.

45. A method of processing nonbinding oilseeds comprising the steps of:

extracting a first portion of the oilseeds in an alkaline environment to produce a solubilized protein solution;

extracting a second portion of the oilseeds in an acidic environment to produce a dispersed protein slurry;

mixing the solubilized protein solution and dispersed protein slurry; and

passing the mixture over an ultrafiltration membrane having a molecular weight cutoff of at least about 100,000 to separate out color causing and taste causing components and to produce a permeate and a retentate, the retentate having a substantially bland taste and a colorless appearance when dry.

46. A process according to claim 45 wherein the ratio of the amount of oilseeds in the first and second portions is set in order to control the percentage of protein in the retentate.