Process for separation of carbohydrates
First Claim
1. A process for separation of mucopolysaccharides which comprises separating a mucopolysaccharide into its constituent components comprisingcontacting said mucopolysaccharide with a chromatographic column comprising a support selected from the group consisting of crosslinked agarose, non-cross-linked agarose, and a polyvinyl alcohol, said support containing hydrophobic ligands selected from the group consisting of alkyl groups;
- alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group;
alkylene groups substituted with a hydroxyl group;
aryl groups; and
alalkyl groups, and thereby separating said mucopolysaccharide into its constituent components.
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Abstract
There is provided a process for the separation of carbohydrates by use of hydrophobic interaction chromatography.
The process according to the present invention can accomplish effective separation of carbohydrates, particularly mucopolysaccharides including heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronic acid, keratan sulfate and chondroitin-polysulfates. As the hydrophobic ligand in the chromatographic support to be used in the present invention, there may particularly be mentioned and alkyl group; an alkyl group substituted with a hydroxyl group, a carboxyl group and/or an amino group; an aryl group; or an aralkyl group. The chromatographic support is selected from cross-linked and non-cross-linked agaroses and polyvinyl alcohols.
55 Citations
12 Claims
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1. A process for separation of mucopolysaccharides which comprises separating a mucopolysaccharide into its constituent components comprising
contacting said mucopolysaccharide with a chromatographic column comprising a support selected from the group consisting of crosslinked agarose, non-cross-linked agarose, and a polyvinyl alcohol, said support containing hydrophobic ligands selected from the group consisting of alkyl groups; - alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group;
alkylene groups substituted with a hydroxyl group;
aryl groups; and
alalkyl groups, and thereby separating said mucopolysaccharide into its constituent components. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group;
Specification