Apparatus for liquid-solid column centrifugation chromatography and method

• US 4,422,941 A
• Filed: 09/08/1980
• Issued: 12/27/1983
• Est. Priority Date: 09/08/1980
• Status: Expired due to Term

• Current Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Original Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Inventors: Andersen, Klaus B., Vaughan, Maurice H. Jr.
• Primary Examiner(s): Adee, John
• Application Number:
  US06/185,335
• Time in Patent Office: 1,205 Days
• Field of Search: 210/656, 210/657, 210/198.2, 55/386, 233/28, 233/14 A
• US Class Current: 210/657
• CPC Class Codes: B01D 15/18 relating to flow patterns
• CPC Class Codes: B01J 20/281 Sorbents specially adapted for preparative, analytical or investigative chromatography
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B04B 5/00 Other centrifuges
• CPC Class Codes: B04B 5/0414 comprising test tubes
• CPC Class Codes: G01N 2030/381 centrifugal chromatography
• CPC Class Codes: G01N 30/38 Flow patterns
• CPC Class Codes: G01N 30/466 with separation columns in parallel
• CPC Class Codes: G01N 30/6017 Fluid distributors
• CPC Class Codes: G01N 30/603 retaining the stationary phase, e.g. Frits
• CPC Class Codes: G01N 30/6047 with supporting means; Holders

Abstract

Centrifugation chromatography columns including column members for containing a particle bed and a porous member underlying the bed. The column members have an upper receiving opening and a lower discharge opening. A receptacle is demountably secured to the column. Attachment is effected through cooperation between a lower portion of the column members and an upper portion of the receptacles. Support structures may be provided on the exterior of the column members to facilitate support thereof by a centrifuge. In another embodiment of the invention continuous column centrifugation chromatography is provided by means of a column rotor on which are mounted a plurality of columns. A collector rotor is provided with means for receiving material discharged from the columns. Relative synchronized movement between the column rotor and the collector rotor is provided. A distribution system for supplying liquid to be separated to the columns on a continuous basis is provided. Funnel means may be employed to facilitate transfer of effluent from the column to the collector rotor. A method for continuous column centrifugation chromatography employing a rotating array of columns which are subjected to a centrifugal force, effecting synchronized movement of an array of receptacles positioned to receive the liquid discharged from the columns and supplying on a continuous basis liquid to the columns.

Claims

1. Column centrifugation chromatography apparatus comprising

column rotor means for supporting and applying centrifugal force to a plurality of circumferentially spaced nonhelical centrifugation columns,

said centrifugation columns being generally radially oriented,

collector rotor means for collecting material discharged from said columns,

a plurality of receptacles secured to said collector rotor means for receiving liquid discharged from said column,

said receptacles disposed radially outwardly of said columns,

power means for establishing synchronized relative movement of said column rotor means and said collector rotor means,

said column rotor means and said collector rotor means being mounted for substantially coaxial rotation, and

distribution means for supplying liquid to said columns while said column rotor means is subjected to said movement.

2. The column centrifugation chromatography apparatus of claim 1 wherein

at least one of said column rotor means and said collector rotor means being secured to a drive shaft, and

said power means including motor means for rotating said drive shaft.

3. The column centrifugation chromatography apparatus of claim 1 wherein

said distribution means including reservoir means mounted for rotation with said column rotor means and

means connecting said reservoir with said columns.

4. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means are supported by post means disposed generally on the axis of rotation of said column rotor means.

5. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means has an open upper portion for receipt of liquid, and

supply conduit means for delivering said liquid to said reservoir.

6. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means being closed, and

supply conduit means sealingly connected to said reservoir and delivering liquid thereto.

7. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means has a unitary storage chamber.

8. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means having a number of chambers.

9. The column centrifugation chromatography apparatus of claim 3 wherein

said reservoir means has a generally annular chamber.

10. The column centrifugation chromatography apparatus of claim 8 wherein

said reservoir means has a number of annular generally concentric chambers.

11. The column centrifugation chromatography apparatus of claim 1 wherein

said distribution means include tube means for delivery of liquid to said columns, and

stopper means in communication with said tube means and said columns.

12. The column centrifugation chromatography apparatus of claim 11 wherein

said stopper means include a stopper having one passageway in communication with said tube means, and

said passageway branching into a number of subpassageways for discharge of said liquid to several portions of the interior of said column.

13. The column centrifugation chromatography apparatus of claim 1 wherein

said collector rotor means have an annular array of said receptacles.

14. The column centrifugation chromatography apparatus of claim 1 wherein

said receptacles are fixedly mounted with their openings facing generally upwardly and radially inwardly with respect to the center of said collector rotor means.

15. The column centrifugation chromatography apparatus of claim 14 wherein

said receptacles have mouths which diverge generally

radially inwardly with respect to the longitudinal axis of said receptacles.

16. The column centrifugation chromatography apparatus of claim 1 wherein

funnel means are interposed between said columns and said receptacles for facilitating efficient transfer of liquid from said columns to said receptacles.

17. The column centrifugation chromatography apparatus of claim 16 wherein

said funnel means include an annular member having a plurality of generally radially oriented openings, and

said openings diverge generally radially inwardly.

18. The column centrifugation chromatography apparatus of claim 1 wherein

said receptacles are formed integrally within an annular member.

19. The column centrifugation chromatography apparatus of claim 1 wherein

said receptacles are rotatably secured to said collector rotor means, whereby application of centrifugal force thereto will result in the receptacles rotating generally outwardly and upwardly.

20. The centrifugation chromatography apparatus of claim 1 including

said column rotor means having means for supporting chromatography columns, and

means for effecting rotation of said columns about their longitudinal axis.

21. A method of continuous column centrifugation chromatography comprising

providing an annular array of generally radially oriented columns,

applying a centrifugal force to said array of columns through rotation of said array,

effecting synchronized relative movement of an array of receptacles positioned generally radially outwardly of said columns to receive liquid discharge from said columns, and

supplying on a continuous basis liquid to said centrifuge columns.

22. The method of chromatography of claim 21 including

providing chromatographic bed material in said columns, and

removing effluent from said columns on a substantially continuous basis.