Enhanced growth medium and method for culturing human mammary epithelial cells

• US 4,423,145 A
• Filed: 05/07/1981
• Issued: 12/27/1983
• Est. Priority Date: 05/07/1981
• Status: Expired due to Fees

• Current Assignee: Peralta Cancer Research Institute, Peralta Hospital Association
• Original Assignee: Adeline J. Hackett, Helene S. Smith, Martha R. Stampfer
• Inventors: Stampfer, Martha R., Smith, Helene S., Hackett, Adeline J.
• Primary Examiner(s): Wiseman, Thomas G.
• Assistant Examiner(s): Tarcza, John E.
• Application Number:
  US06/261,086
• Time in Patent Office: 964 Days
• Field of Search: 435/29, 435/240, 435/241, 435/32, 435/201, 435/219, 435/267, 435/948, 23/230 B, 436/64, 436/65
• US Class Current: 435/32
• CPC Class Codes: C12N 2501/01 Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
• CPC Class Codes: C12N 2501/33 Insulin
• CPC Class Codes: C12N 2502/23 Gastro-intestinal tract cells
• CPC Class Codes: C12N 2502/253 Bladder cells
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: C12N 2509/00 Methods for the dissociation of cells, e.g. specific use of enzymes
• CPC Class Codes: C12N 5/0631 Mammary cells
• CPC Class Codes: G01N 33/5014 for testing toxicity
• CPC Class Codes: Y10S 435/948 using viruses or cell lines

Abstract

Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.

Claims

1. A method for preparing samples of human mammary tissue to obtain a mass culture of mammary epithelial cells, said method comprising:

digesting the tissue samples with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells;

separating the clumps of epithelial cells from the stromal cells and other cellular material; and

,

culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the mammary epithelial cells in the clumps proliferate.

2. A method for passaging the epithelial cells obtained by the steps of claim 1 to obtain a clonal culture, said method comprising the additional steps of:

separating individual cells from the clumps of epithelial cells; and

plating individual cells onto a substrate in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies.

4. A method as in claim 1, wherein the step of digesting the tissue is carried out with agitation and at a temperature in the range from approximately 20°

to 37°

C. and wherein said digestion step is terminated when clumps of the cells are observed to be formed.

5. A method as in claim 2, wherein the step of separating individual cells from the clumps of epithelial cells comprises treating the clumps with a mixture including trypsin.

6. A method as in claim 2, wherein the substrate is plated with feeder cells prior to culturing the epithelial cells.

7. A method as in claim 6, wherein the feeder cells are normal human skin fibroblasts.

3. An assay for determining the toxicity of a particular agent to human mammary epithelial cells, wherein viable colonies of human mammary epithelial cells are prepared by:

digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells;

separating the clumps of epithelial cells from the stromal cells and other cellular material; and

,

culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate;

separating individual cells from the clumps of epithelial cells; and

plating a relatively small number of the individual cells onto a substrate in a medium including conditioned media from at least one media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies;

said assay comprising;

exposing the viable colonies to the particular agent; and

determining the survival rate of the colonies.

8. An assay for determining the toxicity of a particular agent to human mammary epithelial cells, said assay employing a culture of human mammary epithelial cells prepared by:

digesting samples of human mammary tissue with an enzyme digestion mixture including at least one enzyme selected to break down the mammary tissue into clumps of epithelial cells substantially free from attached stromal cells;

separating the clumps of epithelial cells from the stromal cells and other cellular material; and

culturing the clumps of epithelial cells in a medium including conditioned media obtained from cultures of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells so that the cells in the clumps proliferate;

separating individual cells from the clumps of epithelial cells; and

plating individual cells onto a substrate in a medium including conditioned media from at least one of the media obtained from human fetal intestine epithelial cells and human bladder epithelial cells so that the cells proliferate in isolation from one another to form colonies, wherein said substrate has been plated with feeder cells prior to culturing the epithelial cells;

said assay comprising;

exposing the plated cells to the agent;

terminating the exposure of the colonies to the treatment;

providing supplemental growth media further including feeder cells;

allowing the colonies plated on the substrate to proliferate until it is possible to distinguish those colonies which continue to grow from those colonies which have ceased to grow prior to determining the survival rate; and

determining the toxicity by comparing the number of viable colonies with the number of colonies which have ceased to grow.

9. A growth medium, comprising insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal intestine epithelial cells and human bladder epithelial cells, said components being present in basal medium at concentrations effective to promote the growth of human mammary epithelial cells therein.

10. A growth medium, as in claim 9 wherein the insulin is present at a concentration in the range from approximately 0.1 to 20 μ

g/ml.

11. A growth medium, as in claim 9 wherein the cholera toxin is present at a concentration in the range from approximately 0.1 to 1000 ng/ml.

12. A growth medium, as in claim 9 wherein the conditioned media is present at a concentration in the range from approximately 10 to 50 weight percent.

13. A growth medium, as in claim 9 including additional components selected from the group consisting of epidermal growth factor at a concentration not exceeding approximately 20 ng/ml, triiodothyronine at a concentration not exceeding approximately 10^-7 M, estradiol at a concentration not exceeding 10^-7 M, and serum not exceeding 3% by weight.

14. A viable culture of human mammary epithetial cells in a basal medium comprising said cells, insulin, cholera toxin and conditioned medium obtained from a culture of cells selected from the group consisting of human fetal instestine epithelial cells and human bladder epithelial cells wherein said insulin, cholera toxin and conditioned medium are present in amounts sufficient to promote the growth of human mammary epithetial cells.