Analytical element

• US 4,427,632 A
• Filed: 04/22/1982
• Issued: 01/24/1984
• Est. Priority Date: 04/29/1981
• Status: Expired due to Term

• Current Assignee: Konishiroku Photo Industry Co., Ltd. (Konica Minolta Inc.)
• Original Assignee: Konishiroku Photo Industry Co., Ltd. (Konica Minolta Inc.)
• Inventors: Koyama, Mikio, Kikugawa, Shozo, Okaniwa, Kenichiro
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/370,854
• Time in Patent Office: 642 Days
• Field of Search: 422/56, 422/57
• US Class Current: 422/423
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements

Abstract

An analytical element which comprises (a) a light-transmissive and liquid-impermeable support, (b) at least one reagent layer containing at least one reagent reactive with a component in a liquid sample and being constituted of a hydrophilic colloid, and (c) at least one developing layer of a fibrous structure being positioned on said reagent layer on the side opposite to said support for permitting the component in said liquid sample to permeate therethrough toward said reagent layer and having been formed by coating said reagent layer with a dispersion comprising an organic macromolecular polymer having reactive groups and a fibrous material.

Claims

1. An analytical element for determining the presence of a component in a liquid sample comprising:

(a) a light-transmissive and liquid-impermeable support;

(b) at least one reagent layer containing at least one reagent reactive with said component and containing a hydrophilic colloid; and

(c) at least one hydrophobic developing layer having a fibrous structure and being positioned on the side of said reagent layer opposite to said support whereby the component in said liquid sample permeates through said developing layer toward said reagent layer, said developing layer formed by coating said reagent layer with a homogeneous dispersion comprising a hydrophobic organic macromolecular polymer having reactive groups and a fibrous material.

2. The analytical element of claim 1, wherein said dispersion contains said hydrophobic organic macromolecular polymer having reactive groups in an amount of 0.005 to 50% by weight based on said fibrous material.

3. The analytical element of claim 1, wherein said reactive groups of the hydrophobic organic macromolecular polymer are selected from the group consisting of epoxy, aziridyl, formyl, hydroxymethyl, isocyanate, thiol and carbamoyl groups.

4. The analytical element of claim 1, wherein said fibrous material for the developing layer is selected from the group consisting of natural celluloses, natural cellulose derivatives, polyethylenes, polypropylenes and polyamides.

5. The analytical element of claim 1, wherein said developing layer has a thickness of 30 to 600 microns.

6. The analytical element of claim 1, wherein said dispersion contains at least one surfactant.

7. The analytical element of claim 6, wherein said dispersion contains said surfactant in an amount of 0.005 to 30% by weight based on said fibrous material.

8. The analytical element of claim 1, wherein said support has a thickness of about 50 to 250 microns.

9. The analytical element of claim 8, wherein said support comprises a compound selected from cellulose acetates, polyethyleneterephthalates, polycarbonates and polystyrenes.

10. The analytical element of claim 1, wherein said hydrophilic colloid is selected from the group consisting of gelatins, gelatin derivatives, polyvinyl alcohols and polyvinyl pyrrolidones.

11. The analytical element of claim 10, wherein said hydrophilic colloid is gelatin.

12. The analytical element of claim 10, wherein said hydrophilic colloid has a film thickness of at least about 5 microns.

13. The analytical element of claim 1, wherein said hydrophobic organic macromolecular polymer consists essentially of a copolymer having at least one monomer having reactive groups and at least one monomer selected from the group consisting of:

##STR2## wherein each of R¹ and R², which can be the same or different, is a non-interfering substituent selected from a hydrogen atom, a halogen atom, and a substituted or unsubstituted amino-free alkyl or aryl group having 1 to 10 carbon atoms and R³ is selected from a hydrogen atom, a halogen atom, or a substituted or unsubstituted, amino-free aliphatic or aromatic group having 1 to 10 carbon atoms;

(II)

space="preserve" listing-type="equation">CHR.sup.6 ═

CR.sup.4 --COOR.sup.5

wherein R⁶ has the same meaning as R¹ in formula (I), R⁴ is selected from the group consisting of a hydrogen atom and a methyl group and R⁵ is selected from the group consisting of an aryl, an alkyl, an alkaryl and aralkyl group, each having 1 to 10 carbon atoms;

(III) polymerizable unsaturated nitrile monomers; and

(IV) interparticle crosslinking monomers having two addition-polymerizable groups.

14. The analytical element of claim 13, wherein the copolymer is comprised of 0.1 to 30% by weight of the monomer having reactive groups, 0 to 99.5% by weight of the monomer (I), 0 to 99.5% by weight of the monomer (II), 0 to 99.5% by weight of the monomer (III) and 0 to 10% by weight of the monomer (IV).

15. The analytical element of claim 13, wherein the monomer having reactive groups is present in an amount of 0.1 to 30% by weight based on said hydrophobic organic macromolecular polymer.

16. The analytical element of claim 15, wherein the monomer having reactive groups is present in an amount of 0.5 to 20% by weight based on said hydrophobic organic macromolecular polymer.

17. An analytical element for determining the presence of a component in a liquid sample comprising:

(a) a light-transmissive and liquid-impermeable support selected from cellulose acetates, polyethyleneterephthalates, polycarbonates and polystyrenes;

(b) at least one reagent layer containing at least one reagent reactive with said component and containing a hydrophilic colloid selected from the group consisting of gelatins, gelatin derivatives, polyvinyl alcohols and polyvinyl pyrrolidones, said hydrophilic colloid having a film thickness of at least about 5 microns; and

(c) at least one hydrophobic developing layer having a fibrous structure and being positioned on the side of said reagent layer opposite to said support whereby the component in said liquid sample permeates through said developing layer toward said reagent layer, said developing layer formed by coating said reagent layer with a homogeneous dispersion comprising a hydrophobic organic macromolecular polymer having reactive groups and a fibrous material, said hydrophobic organic macromolecular polymer consisting essentially of a copolymer having at least one monomer having reactive groups and at least one monomer selected from the group consisting of;

##STR3## wherein each of R¹ and R², which can be the same or different, is a non-interfering substituent selected from a hydrogen atom, a halogen atom, and a substituted or unsubstituted amino-free alkyl or aryl group having 1 to 10 carbon atoms and R³ is selected from a hydrogen atom, a halogen atom, or a substituted or unsubstituted, amino-free aliphatic or aromatic group having 1 to 10 carbon atoms;

(II)

space="preserve" listing-type="equation">CHR.sup.6 ═

CR.sup.4 -COOR.sup.5

wherein R⁶ has the same meaning as R¹ in formula (I), R⁴ is selected from the group consisting of a hydrogen atom and a methyl group and R⁵ is selected from the group consisting of an aryl, an alkyl, an alkaryl and aralkyl group, each having 1 to 10 carbon atoms;

(III) polymerizable unsaturated nitrile monomers; and

(IV) interparticle crosslinking monomers having two addition-polymerizable groups, wherein the monomer having reactive groups is present in an amount of 0.1 to 30% by weight based on said hydrophobic organic macromolecular polymer.