Method and system for externally treating the blood

• US 4,428,744 A
• Filed: 06/16/1981
• Issued: 01/31/1984
• Est. Priority Date: 12/11/1979
• Status: Expired due to Term

• Current Assignee: Eleanor F. Bourke, Frederic A. Bourke, Jr., Richard L. Edelson, The Edelson Trust
• Original Assignee: Eleanor F. Bourke, Frederic A. Bourke, Jr., Richard L. Edelson, The Edelson Trust
• Inventors: Edelson, Richard L.
• Primary Examiner(s): Lesmes, George F.
• Assistant Examiner(s): Swisher, Nancy A. B.
• Application Number:
  US06/274,319
• Time in Patent Office: 959 Days
• Field of Search: 128/214 B, 128/275.1, 128/207.22, 128/214 R, 128/214.2, 128/1.1, 128/1 R, 128/395, 128/DIG. 3, 604/6, 604/20, 604/28
• US Class Current: 604/6.08
• CPC Class Codes: A61K 41/10 Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person
• CPC Class Codes: A61M 1/3616 Batch-type treatment
• CPC Class Codes: A61M 1/3623 Means for actively controlling temperature of blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3686 by removing photoactive agents after irradiation
• CPC Class Codes: Y10S 128/03 Heart-lung

Abstract

A method and system are disclosed for externally treating human blood, with the objective of reducing the functioning lymphocyte population in the blood system of a human subject. According to the method, blood is withdrawn from the subject and passed through an ultraviolet radiation field in the presence of from about 1 nanogram to 100 micrograms per ml of blood, of a dissolved photoactive agent capable of forming photo-adducts with lymphocytic-DNA, mobile cortisone receptors or antigen sites to thereby effect covalent bonding between the photoactive agent and the same, thereby inhibiting the metabolic processes of the lymphocytes or complexing them; and thereupon returning the irradiated blood to the subject. The withdrawn blood may be formed into an extracorporeal stream and flowed through a treatment station whereat the irradiation is effected, as for example by exposure to UV radiation; and such flow process may be conducted on a continuous basis. If desired, at least portions of the treated blood may then be separated, as for example by a continuous centrifuge, before returning the remaining diverted blood to the subject.

Claims

1. A method for reducing the functioning population of nucleated cells in the blood supply of a human subject, comprising the steps of:

withdrawing blood from said subject, and irradiating said withdrawn blood with UV radiation in the presence of an effective amount of a dissolved photoactive chemical agent having an affinity for the nucleic acid of the nucleated blood cells and capable when activated by said UV radiation of forming photo-adducts with DNA, to thereby effect chemical bonding between said photoactive chemical agent and the nucleic acid of the nucleated cells, thereby altering the said nucleic acid and inhibiting the metabolic processes of said nucleated cells; and

returning the irradiated blood to said subject.

2. A method in accordance with claim 1 wherein the nucleated cells are lymphocytes and said photoactive chemical agent is a psoralen.

3. A method in accordance with claim 1, wherein said withdrawn blood is formed into an extracorporeal flowing stream, passed through a treatment station whereat said irradiation is effected and returned to said subject in a continuous operation.

4. A method in accordance with claim 1, including the further step of separating at least portions of said nucleated cell population before returning said blood to said subject.

5. A method in accordance with claim 1, including the further step of separating at least portions of said nucleated cell population before returning said blood to said subject, by passing said bloodstream through a continuous flow centrifuge.

6. A method in accordance with claim 5 wherein excess quantities of said photoactive chemical agent are removed from the extracorporeal stream by withdrawing blood plasma at said centrifuge and replacing same with fresh photoactive chemical agent-free plasma.

7. A method in accordance with claim 1 wherein said blood is irradiated with photoenergy in the UVA wavelength range and at a radiation dose level of from about 0.1 to 100 joules/cm².

8. A method in accordance with claim 1 wherein said photoactive chemical agent is dissolved in said blood by mixing from about 1 nanogram to 100 micrograms of the same per ml of blood subsequent to withdrawing the blood from said subject.

9. A method in accordance with claim 1 wherein said photoactive chemical agent is dissolved in said blood by administering said agent to said subject.

10. A method for reducing the population of lymphocytes with intense metabolic activity in the blood supply of a human subject, comprising the steps of:

withdrawing blood from said subject, forming said blood into an extracorporeal stream, flowing said stream through a treatment station, irradiating said withdrawn blood in said treatment station with UV radiation to the presence of about 1 nanogram to 100 micrograms of a dissolved psoralen capable when activated by said UV radiation to effect chemical bonding between said psoralen and said lymphocytes, thereby selectively inhibiting the metabolic processes of said lymphocytes; and

returning the irradiated blood to said subject.

11. The method of claim 10 including passing the blood from said extracorporeal stream through a centrifuge and separating out portions of said blood before returning the irradiated blood to said subject.

12. The method of claim 11 wherein said process is carried out continuously.