Enzyme electrode

• US 4,431,507 A
• Filed: 01/12/1982
• Issued: 02/14/1984
• Est. Priority Date: 01/14/1981
• Status: Expired due to Term

• Current Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Iijima, Takashi, Imai, Akihiro, Nankai, Shiro
• Primary Examiner(s): Williams, Howard S.
• Assistant Examiner(s): Boggs, Jr., B. J.
• Application Number:
  US06/338,957
• Time in Patent Office: 763 Days
• Field of Search: 204/195 B, 204/195 P, 204/1 E, 204/403, 435/817
• US Class Current: 204/403.11
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The disclosure is directed to an improved enzyme electrode which includes a first electrode having at least one kind of an enzyme immobilized on it for electrochemically detecting a substance to be produced in association with a reaction based on the enzyme, and a second electrode for electrochemically removing materials which interfere with the detection by the first electrode. The second electrode is disposed at the side of a test solution containing a substrate of the enzyme with respect to the first electrode.

Claims

1. An enzyme electrode assembly which comprises a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being disposed at the side of a test solution containing a substrate of said enzyme with respect to said first electrode.

2. An enzyme electrode assembly as claimed in claim 1, wherein said second electrode is mode of a thin layer formed selectively of a metal or metal oxide on a porous membrane.

3. An enzyme electrode assembly as claimed in claim 1 or 2, wherein said first electrode is made of by a porous membrane having a thin layer of a metal or metal oxide formed on its surface, with the enzyme being immobilized on said porous membrane.

4. An enzyme electrode assembly as claimed in claim 3, wherein said first electrode and second electrode are laminated one upon another.

5. An enzyme electrode assembly as claimed in claim 3, wherein said first electrode is formed at one side of the porous membrane, with said second electrode being formed at the other side of said porous membrane.

6. An electrode assembly comprising a first electrode including at least one enzyme immobilized thereon for electrochemically detecting a substance to be produced in association with a reaction based on said enzyme, said enzyme electrode being made of a porous membrane having a thin layer of a metal or metal oxide layer on one surface and at least one enzyme immobilized on its opposite surface and a second electrode for electrochemically removing materials which interfere with said detection by said first electrode, said second electrode being also composed of a porous membrane with a thin layer of a metal or metal oxide on one side of the porous membrane;

said first and second electrodes being laminated such that the immobilized enzyme(s) is sandwiched between between the two porous membranes and the metal or metal oxide layers are located on the outer portions of the laminated structure.

7. An electrode assembly comprising a porous membrane having first a metal or metal oxide electrode layer located on one side with an enzyme immobilized on said layer and within the pores of the membrane to constitute a first electrode and a second electrode metal and metal oxide layer located in the opposite side of the porous membrane, said first electrode-functioning to detect a substance to be produced in association with an enzyme reaction, based on said enzyme and said second electrode functioning to electrochemically remove materials which interfere with said detection by the first electrode.