Tridecadeoxynucleotide, process for preparation thereof, and use thereof

• US 4,433,140 A
• Filed: 02/24/1982
• Issued: 02/21/1984
• Est. Priority Date: 02/25/1981
• Status: Expired due to Fees

• Current Assignee: Dr. Karl Thomae GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Dr. Karl Thomae GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Seliger, Hartmut, Swetly, Peter, Rastl, Eva
• Primary Examiner(s): Love, Ethel G.
• Application Number:
  US06/351,851
• Time in Patent Office: 727 Days
• Field of Search: 536/27, 536/28, 536/29
• US Class Current: 536/24.33
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07K 14/555 Interferons [IFN]

Abstract

A tridecadeoxynucleotide of the formula

dCCTTCTGGAACTG,

is useful as a primer in the enzymatic synthesis of deoxyribonucleic acid (DNA) on messenger ribonucleic acid (mRNA) matrices.

The new tridecadeoxynucleotide is obtained by linking suitable partially protected oligonucleotide blocks, particularly two suitable oligonucleotide fragments, e.g. by linking a suitable tetramer to a suitable nomaer, using a condensing agent.

Claims

1. A tridecadeoxynucleotide of the formula dCCTTCTGGAACTG in substantially pure form.