Immunological reagents employing polymeric backbone possessing reactive functional groups
First Claim
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1. An immunological substance detecting reagent comprising:
- (a) an immunological homolog specific for the substance to be detected;
(b) a water-soluble, substantially noncross-linked and nonprimary amine containing polymer having a net charge not greater than zero; and
(c) means for attaching substantially only one homolog to each water-soluble polymer.
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Abstract
An immunological substance detecting reagent is provided which, in the preferred embodiment, employs an immunological homolog specific for the immunological substance to be detected coupled to a water-soluble polymer having a net charge not greater than zero. The water-soluble polymer further has associated a plurality of marker substances such as fluorophore molecules thereby providing a reagent of increased sensitivity for the detection of an antigen-antibody immunological reaction.
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Citations
16 Claims
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1. An immunological substance detecting reagent comprising:
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(a) an immunological homolog specific for the substance to be detected; (b) a water-soluble, substantially noncross-linked and nonprimary amine containing polymer having a net charge not greater than zero; and (c) means for attaching substantially only one homolog to each water-soluble polymer. - View Dependent Claims (4, 5, 7, 13)
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2. An immunological substance detecting reagent comprising:
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(a) an immunological homolog specific for the substance to be detected; (b) a water-soluble polymer-marker substance complex having in combination a net charge not greater than zero, said polymer being nonprimary amine containing and substantially noncross-linked; and (c) means for attaching substantially only one homolog to each water-soluble polymer. - View Dependent Claims (6)
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3. An immunological substance detecting reagent comprising:
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(a) an immunological homolog specific for the substance to be detected; and (b) a water-soluble polymer having a marker substance associated and means for attaching substantially only one homolog to each polymer, the entire polymer-marker-attaching means complex having a net charge not greater than zero, said polymer being nonprimary amine containing and substantially noncross-linked.
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8. An immunological substance detecting reagent comprising:
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(a) a first immunological homolog specific for the substance to be detected; (b) a water-soluble, nonprimary amine containing and substantially noncross-linked polymer having a charge not greater than zero and selected from the group consisting of polyacrylic acid, polymethacrylic acid, polyacrylamide, polyvinylalcohol, polyallylalcohol, polymer combinations of the foregoing, hydroxyethyl cellulose, hydroxypropyl cellulose, natural water-soluble polymers and synthetic water-soluble polymers; and
the polymer further having in association therewith a marker substance selected from the group consisting of nonfluorescent dyes, fluorescent dyes, radioisotopes, electron opaque substances, enzymes, a second immunological homolog of differing specificity than the first homolog and microspheres; and(c) means for attaching substantially only one first homolog to each polymer-marker complex.
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9. An immunological substance detecting reagent comprising:
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(a) a first immunological homolog specific for the substance to be detected; (b) a water-soluble, nonprimary amine containing and substantially noncross-linked polymer selected from the group consisting of polyacrylic acid, polymethacrylic acid, polyacrylamide, polyvinylalcohol, polyallylalcohol, polymer combinations of the foregoing, hydroxyethyl cellulose, hydroxypropyl cellulose, natural water-soluble polymers and synthetic water-soluble polymers, and having in association therewith a marker substance selected from the group consisting of nonfluorescent dyes, fluorescent dyes, radioisotopes, electron opaque substances, enzymes, a second immunological homolog of differing specificity than the first homolog and microspheres, the polymer-marker complex having a net charge not greater than zero; and (c) means for attaching substantially only one first homolog to each polymer-marker complex.
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10. An immunological substance detecting reagent comprising:
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(a) a first immunological homolog specific for the substance to be detected; and (b) a water-soluble, nonprimary amine containing and substantially noncross-linked polymer, selected from the group consisting of polyacrylic acid, polymethacrylic acid, polyacrylamide, polyvinyl alcohol, polyallylalcohol, polymer combinations of the foregoing, hydroxyethyl cellulose, hydroxypropyl cellulose, natural water-soluble polymers and synthetic water-soluble polymers, the polymer having in association therewith a marker substance selected from the group consisting of nonfluorescent dyes, fluorescent dyes, radioisotopes, electron opaque substances, enzymes, a second immunological homolog of differing specificity than the first homolog and microspheres, and the polymer further having in combination therewith means for attaching substantially only one first homolog to each polymer, the entire polymer-marker-attaching means complex having a net charge not greater than zero. - View Dependent Claims (11, 12)
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14. A treatment agent specific for tissue having an associated antigen comprising:
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(a) a water-soluble, nonprimary amine containing and substantially noncross-linked polymer having a net charge not greater than zero; (b) an antibody, specific for the antigen, bound to the polymer on a substantially one antibody per polymer basis; and (c) a therapeutic substance bound to the polymer and selected from the group consisting of chemotherapeutic substances, radiotherapeutic substances, vitamins, tumor-inhibiting drugs, and tissue affecting materials desired to be delivered to the antigenic site. - View Dependent Claims (15, 16)
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Specification