Mechanically actuated pipette dispenser

• US 4,437,586 A
• Filed: 03/29/1982
• Issued: 03/20/1984
• Est. Priority Date: 03/29/1982
• Status: Expired due to Fees

• Current Assignee: Eastman Kodak Company
• Original Assignee: Eastman Kodak Company
• Inventors: Columbus, Richard L.
• Primary Examiner(s): Rolla, Joseph J.
• Assistant Examiner(s): Huppert, Michael S.
• Application Number:
  US06/362,931
• Time in Patent Office: 722 Days
• Field of Search: 73/864.15, 73/864.16, 73/864.23, 604/124, 604/125, 604/207, 604/208, 604/209, 604/210, 604/184, 604/224, 604/231, 222/43, 222/185, 222/309, 222/333, 222/386, 222/391, 222/180, 222/181
• US Class Current: 222/181.2
• CPC Class Codes: B01L 3/0234 Repeating pipettes, i.e. for dispensing multiple doses from a single charge
• CPC Class Codes: B01L 9/54 Supports specially adapted for pipettes and burettes automated pipetting stations G01N35/10

Abstract

A liquid dispenser is disclosed, comprising a vented pipette and an actuator for the pipette. The actuator includes apparatus for mounting the pipette and a mechanism for automatically lowering a piston into the pipette to a predetermined starting level before the pipette vent is sealed, to insure that a predictable quantity of liquid is dispensed once the vent is sealed.

Claims

1. In a dispenser including

a pipette comprising means at a first end for containing liquid for dispensing, a compression chamber in fluid communication with said first end, compressing means, including a piston, for providing fluid compression within said chamber, and means for venting said chamber to the atmosphere, and

actuating means including mounting means for receiving said pipette and for removably securing the compression chamber of said pipette in a fixed position and means for engaging and moving said compressing means by a predetermined distance to dispense a fixed amount of liquid;

the improvement wherein said actuating means includes moving and sealing means, operable during receipt of said pipette, for sequentially;

(1) moving said piston with said chamber in a vented condition, to a predetermined starting position relative to said mounting means while said actuating means is stationary with respect to said compression chamber, and (2) thereafter sealing said venting means,

whereby said piston is automatically initialized without dispensing any of the liquid contained by said pipette.

2. A dispenser as defined in claim 1, wherein said venting means comprises a vent aperture extending through said piston to an end of said pipette that is opposite said first end,

and wherein said actuating means comprises a cantilevered piston-actuation arm having a camming surface and a sealing surface adapted to seal against said vent aperture when said pipette is fully mounted in said actuating means, said camming surface extending from the cantilevered end of said actuation arm to said sealing surface,

said camming surface having a groove extending from said cantilevered end to a location adjacent said sealing surface, said groove being positioned to expose to the atmosphere said vent aperture as said pipette is being mounted in said actuating means and lowered to said starting position by said camming surface.

3. A dispenser as defined in claim 2, and further including incrementing means for moving in increments said actuation arm and said piston to provide compression within said chamber, each increment corresponding to a desired volume of dispensed liquid.

4. A dispenser as defined in claim 3, wherein said incrementing means comprises a pawl and ratchet, said actuation arm being operatively connected to a member having said ratchet as a portion thereof.

5. Actuating means for dispensing a fixed amount of liquid from a pipette having means at one end for containing the dispensable liquid, a compression chamber in fluid communication with said containing means, compressing means, including a piston movably mounted within said chamber, for providing compression within said chamber, and means for venting the pipette to the atmosphere;

the actuating means comprising

means for receiving said pipette and for removably mounting said compression chamber in a fixed position,

means for engaging and moving said piston a predetermined distance to dispense a fixed amount of liquid, and

means, operable during receipt of said pipette, for sequentially;

(1) moving said piston with said chamber in a vented condition, to a predetermined starting position relative to said mounting means while said actuating means is stationary with respect to said compression chamber, and (2) thereafter sealing said venting means,

whereby said piston is automatically initialized without dispensing any of the liquid contained by said pipette.

6. Actuating means as defined in claim 5, wherein the pipette venting means comprises a vent aperture extending through said piston to an end of said pipette opposite to said one end,

and wherein said actuating means comprises a cantilevered piston-actuation arm having a camming surface and a sealing surface adapted to seal against said vent aperture when said pipette is fully mounted in said actuating means, said camming surface extending from the cantilevered end of said actuation arm to said sealing surface,

said camming surface having a groove extending from said cantilevered end to a location adjacent said sealing surface, said groove being positioned to expose to the atmosphere said vent aperture as said pipette is being mounted in said actuating means and lowered to said starting position by said camming surface.

7. Actuating means as defined in claim 6, and further including incrementing means for moving in increments said actuation arm and said piston sealed against said sealing surface to compress air within said chamber, each increment corresponding to a desired volume of dispensed liquid.

8. Actuating means as defined in claim 7, wherein said incrementing means comprises a pawl and ratchet, said actuation arm being operatively connected to a member having said ratchet as a portion thereof.