Antibodies to immunogenic peptides and their use to purify human fibroblast interferon

• US 4,438,030 A
• Filed: 03/30/1982
• Issued: 03/20/1984
• Est. Priority Date: 07/25/1980
• Status: Expired due to Fees

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Hunkapiller, Michael W., Korant, Bruce D., Knight, Ernest Jr., Ganfield, David J.
• Primary Examiner(s): Hazel, Blondel
• Application Number:
  US06/363,743
• Time in Patent Office: 721 Days
• Field of Search: 424/85, 260/112 R, 260/112.5 R, 436/536, 436/547
• US Class Current: 530/351
• CPC Class Codes: C07K 14/565 IFN-beta
• CPC Class Codes: C07K 16/249 Interferons
• CPC Class Codes: G01N 33/6866 Interferon
• CPC Class Codes: Y10S 930/142 Interferon

Abstract

Antibodies to the twenty-one acid peptide: (X-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-Y-Gln-Lys-Leu-Leu)_n, where X is Met or Ser, Y is His or Cys and n is 1 to 12; peptide/protein conjugates and antibodies thereto; process for producing the antibodies; and processes for assaying human fibroblast interferon and for purifying it.

Claims

1. A process for purifying human fibroblast interferon contained in a liquid phase mixture of proteins, comprising (i) contacting the protein mixture with antibodies produced in response to the peptide having the amino acid sequence:

space="preserve" listing-type="equation">(X-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-Y-Gln-Lys-Leu-Leu).sub.n

wherein;

X is selected from Met and Ser,

Y is selected from His and Cys, and

n is 1 to 12;

and forming an interferon-antibody complex;

(ii) separating the interferon-antibody complex from the liquid phase; and

(iii) separating the interferon from the antibody.

2. A process for purifying protein and protein fragments of human fibroblast interferon contained in a liquid phase mixture of proteins, said protein and protein fragments containing one or more of the peptide sequences defined in step (i) the process comprising (i) contacting the protein mixture with antibodies produced in response to the peptide having the amino acid sequence:

space="preserve" listing-type="equation">(X-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-Y-Gln-Lys-Leu-Leu).sub.n

wherein;

X is selected from Met and Ser,

Y is selected from His and Cys, and

n is 1 to 12;

and forming a complex of the antibody with the protein or protein fragment of human fibroblast interferon;

(ii) separating the complex from the liquid phase; and

(iii) separating the protein and protein fragments of the interferon from the antibody.