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Bispecific antibody determinants

  • US 4,444,878 A
  • Filed: 12/21/1981
  • Issued: 04/24/1984
  • Est. Priority Date: 12/21/1981
  • Status: Expired due to Term
First Claim
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1. A homogenous sample of identical bispecific antibody determinants, each said determinant comprising two L-H half-molecules linked by disulfide bonds, each said L-H half-molecule being specific for a different antigenic determinant, and comprising at least the F(ab'"'"') portion of a monoclonal IgG antibody,one said antigenic determinant comprising an antigenic site on a solid matrix, whereby said bispecific antibody determinant is capable of being immobilized on said solid matrix by binding to said matrix at said antigenic site,said sample comprising a multilamellar assembly whereinsaid antigenic site on said matrix is a site on a haptenic molecule attached to said matrix,said bispecific antibody determinant is bonded to said haptenic molecule,the other said antigenic determinant comprises a first antigenic site on a first protein molecule, said bispecific antibody determinant being bonded to said protein molecule, andthere is bonded to said first protein molecule, at a second antigenic site on said protein molecule, a second bispecific antibody determinant different from the determinant bonded to said haptenic molecule, each said second determinant comprising two L-H half molecules linked by disulfide bonds, each said L-H half molecule being specific for a different antigenic determinant, one said antigenic determinant being a second antigenic site on said first protein molecule, each said half-molecule comprising at least the F(ab'"'"') portion of a monoclonal IgG antibody.

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