Process for making human antibody producing B-lymphocytes
First Claim
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1. Process for making antibody producing human B-lymphocytes which comprises culturing a human lymphoid cell suspension containing human B-lymphocytes and a cell concentration of about 1×
- 106 to 5×
106 cells/ml in a tissue culture medium containing;
(a) 0.3 to 0.003% antigen;
(b) helper signal producing agents consisting essentially of;
(i) monocytes or conditioned medium containing interleukin 1 derived from said monocytes in an amount to result in a concentration of 0.1 to 30% and(ii) helper T-lymphocytes or helper T-lymphocyte replacing factor obtained from activated T-cells; and
(c) about 1-10% human serum;
and thereafter recovering the antibody producing human B-Lymphocytes from the medium.
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Abstract
Antibody producing human B-lymphocytes are produced by a process which comprises culturing human B-lymphocytes in a tissue culture medium in the presence of an antigen; helper signal producing agents comprising monocytes or monocyte conditioned medium containing Interleukin 1 (I1-1), and helper T-lymphocytes or helper T-lymphocyte replacing factor; and human serum; and thereafter recovering the antibody producing cells from the medium.
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29 Claims
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1. Process for making antibody producing human B-lymphocytes which comprises culturing a human lymphoid cell suspension containing human B-lymphocytes and a cell concentration of about 1×
- 106 to 5×
106 cells/ml in a tissue culture medium containing;(a) 0.3 to 0.003% antigen; (b) helper signal producing agents consisting essentially of; (i) monocytes or conditioned medium containing interleukin 1 derived from said monocytes in an amount to result in a concentration of 0.1 to 30% and (ii) helper T-lymphocytes or helper T-lymphocyte replacing factor obtained from activated T-cells; and (c) about 1-10% human serum; and thereafter recovering the antibody producing human B-Lymphocytes from the medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
- 106 to 5×
Specification