Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex
First Claim
1. A process for separating male and female determining sperm from native semen which comprises:
- (a) treating the native semen with excess mono-specific male-specific antibody free from male-specificity interfering antibodies and coupling reaction interfering proteins to form comjugates between the male determining sperm and the antibodies,(b) coupling anti-immunoglobulin antibody capable of specifically binding said male-specific antibody to a solid phase immunoabsorbent substrate, said substrate having a form capable of being loaded into a separation column,(c) loading a separation column with the conjugate of the solid phase immunoabsorbent and anti-immunoglobulin antibody,(d) passing the treated semen containing male and female determining sperm through the column, whereby the male determining sperm and male-specific antibody conjugates are bound to the solid phase immunoabsorbent, while the female determining sperm passes out of the column, and(e) separating out the male determining sperm from the column.
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Accused Products
Abstract
A method of manufacturing and a raw material and its useage are disclosed for increasing the percentage of mammalian offspring of either sex. The process of use utilizes a male-specific antibody coupled to a solid-phase immunoabsorbent material to effect a separation of sex-determining spermatozoa derived from a semen suspension. The male-specific antibody selectively binds male-determining spermatozoa, while the female-determining spermatozoa remain not bound and are recoverable. A male specific antibody that is monospecific is prepared from male cells subjected to a multistep process which includes using said cells for the hyperimmunization of a female species and the preparation of an antiserum which is purified as to specificity by a multiplicity of absorptions against female cells. The antiserum is fractionated to yield male specific monospecific immunoglobulin which may be used to effect a separation of sex determining spermatozoa.
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Citations
7 Claims
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1. A process for separating male and female determining sperm from native semen which comprises:
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(a) treating the native semen with excess mono-specific male-specific antibody free from male-specificity interfering antibodies and coupling reaction interfering proteins to form comjugates between the male determining sperm and the antibodies, (b) coupling anti-immunoglobulin antibody capable of specifically binding said male-specific antibody to a solid phase immunoabsorbent substrate, said substrate having a form capable of being loaded into a separation column, (c) loading a separation column with the conjugate of the solid phase immunoabsorbent and anti-immunoglobulin antibody, (d) passing the treated semen containing male and female determining sperm through the column, whereby the male determining sperm and male-specific antibody conjugates are bound to the solid phase immunoabsorbent, while the female determining sperm passes out of the column, and (e) separating out the male determining sperm from the column. - View Dependent Claims (2, 3, 4)
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5. As a new composition of matter, an antibody composition containing monospecific male-specific antibody free from specificity interfering antibodies and coupling reaction interfering proteins.
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6. As a new composition, a conjugate of activated solid phase immunoabsorbent material having anti-globulin specific antibody capable of binding male-specific sperm distributed over the surface of said immunoabsorbent material, and coupled thereto
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7. The method of increasing the percentage of mammalian offspring of either sex, comprising the step of selectively separating male-determining spermatozoa by treating a body of semen with male-specific antibody, and contacting the treated semen with anti-globulin antibody capable of binding male-specific sperm, said anti-globulin antibody being coupled to immunoabsorbent material.
Specification