Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex

• US 4,448,767 A
• Filed: 02/15/1980
• Issued: 05/15/1984
• Est. Priority Date: 10/11/1977
• Status: Expired due to Term

• Current Assignee: Sumar Corporation
• Original Assignee: Sumar Corporation
• Inventors: Bryant, Bernard J.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/117,341
• Time in Patent Office: 1,551 Days
• Field of Search: 424/85, 424/105, 435/1, 435/2
• US Class Current: 530/389.1
• CPC Class Codes: A01K 67/02 Breeding vertebrates
• CPC Class Codes: A61K 35/52 Sperm; Prostate; Seminal fluid; Leydig cells of testes
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: A61P 13/02 of urine or of the urinary tract, e.g. urine acidifiers
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: Y10S 424/811 involving sex selection or contraception

Abstract

A method of manufacturing and a raw material and its useage are disclosed for increasing the percentage of mammalian offspring of either sex. The process of use utilizes a male-specific antibody coupled to a solid-phase immunoabsorbent material to effect a separation of sex-determining spermatozoa derived from a semen suspension. The male-specific antibody selectively binds male-determining spermatozoa, while the female-determining spermatozoa remain not bound and are recoverable. A male specific antibody that is monospecific is prepared from male cells subjected to a multistep process which includes using said cells for the hyperimmunization of a female species and the preparation of an antiserum which is purified as to specificity by a multiplicity of absorptions against female cells. The antiserum is fractionated to yield male specific monospecific immunoglobulin which may be used to effect a separation of sex determining spermatozoa.

Claims

1. A process for separating male and female determining sperm from native semen which comprises:

(a) treating the native semen with excess mono-specific male-specific antibody free from male-specificity interfering antibodies and coupling reaction interfering proteins to form comjugates between the male determining sperm and the antibodies,

(b) coupling anti-immunoglobulin antibody capable of specifically binding said male-specific antibody to a solid phase immunoabsorbent substrate, said substrate having a form capable of being loaded into a separation column,

(c) loading a separation column with the conjugate of the solid phase immunoabsorbent and anti-immunoglobulin antibody,

(d) passing the treated semen containing male and female determining sperm through the column, whereby the male determining sperm and male-specific antibody conjugates are bound to the solid phase immunoabsorbent, while the female determining sperm passes out of the column, and

(e) separating out the male determining sperm from the column.

2. The process of claim 1 wherein the step of separating out the male determining sperm comprises passing a composition consisting essentially of monospecific male specific antibody through the column.

3. The process of claim 1 wherein the step of separating the male determining sperm comprises passing a composition consisting essentially of anti-globulin antibody through the column.

4. The process of claim 1 wherein the coupling step (b) comprises:

activating the solid phase immunoabsorbent material to render it capable of accepting a protein by covalent bonding, an

adding a solution of anti-globulin specific antibody thereto.

5. As a new composition of matter, an antibody composition containing monospecific male-specific antibody free from specificity interfering antibodies and coupling reaction interfering proteins.

6. As a new composition, a conjugate of activated solid phase immunoabsorbent material having anti-globulin specific antibody capable of binding male-specific sperm distributed over the surface of said immunoabsorbent material, and coupled thereto

7. The method of increasing the percentage of mammalian offspring of either sex, comprising the step of selectively separating male-determining spermatozoa by treating a body of semen with male-specific antibody, and contacting the treated semen with anti-globulin antibody capable of binding male-specific sperm, said anti-globulin antibody being coupled to immunoabsorbent material.