×

Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex

  • US 4,448,767 A
  • Filed: 02/15/1980
  • Issued: 05/15/1984
  • Est. Priority Date: 10/11/1977
  • Status: Expired due to Term
First Claim
Patent Images

1. A process for separating male and female determining sperm from native semen which comprises:

  • (a) treating the native semen with excess mono-specific male-specific antibody free from male-specificity interfering antibodies and coupling reaction interfering proteins to form comjugates between the male determining sperm and the antibodies,(b) coupling anti-immunoglobulin antibody capable of specifically binding said male-specific antibody to a solid phase immunoabsorbent substrate, said substrate having a form capable of being loaded into a separation column,(c) loading a separation column with the conjugate of the solid phase immunoabsorbent and anti-immunoglobulin antibody,(d) passing the treated semen containing male and female determining sperm through the column, whereby the male determining sperm and male-specific antibody conjugates are bound to the solid phase immunoabsorbent, while the female determining sperm passes out of the column, and(e) separating out the male determining sperm from the column.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×