Water insoluble gel matrix containing lectin bound to antithrombin

• US 4,450,104 A
• Filed: 09/29/1982
• Issued: 05/22/1984
• Est. Priority Date: 09/30/1980
• Status: Expired due to Term

• Current Assignee: Cutter Laboratories, Inc. (Bayer AG)
• Original Assignee: Cutter Laboratories, Inc. (Bayer AG)
• Inventors: Jordan, Robert E.
• Primary Examiner(s): SCHAIN, HOWARD
• Application Number:
  US06/426,560
• Time in Patent Office: 601 Days
• Field of Search: 260/112 R, 260/112 B
• US Class Current: 530/396
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/8128 Antithrombin III
• CPC Class Codes: C08B 37/0075 Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof

Abstract

A lectin-containing, water-insoluble gel matrix with a glycoprotein reversibly bound thereto can be used for fractionating a polysaccharidic substance capable of being separated into components of differing activity by virtue of differing affinity for the glycoprotein. In this manner, heparin can be fractionated into high activity and low activity components.

Claims

1. A composition for fractionating heparin into components of differing activity by virtue of differing affinity for antithrombin, which comprises a lectin-containing, water-insoluble gel matrix having reversibly bound thereto antithrombin specific for gradiently binding the components of differing activity in heparin.

2. The composition of claim 1 which contains an amount of antithrombin sufficient to gradiently bind the components of differing activity.

3. The composition of claim 1 wherein the lectin is Concanavalin A.

4. The composition of claim 1 wherein the water-insoluble gel matrix is an agarose gel.

5. The composition of claim 1 wherein the heparin is unfractionated heparin.