Alcohol removal from blood with alcohol oxidase

• US 4,450,153 A
• Filed: 09/30/1982
• Issued: 05/22/1984
• Est. Priority Date: 09/30/1982
• Status: Expired due to Fees

• Current Assignee: Moldowan Laboratories Incorporated
• Original Assignee: Phillips Petroleum Company (ConocoPhillips)
• Inventors: Hopkins, Thomas R.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/429,758
• Time in Patent Office: 600 Days
• Field of Search: 424/94
• US Class Current: 424/94.4
• CPC Class Codes: A61K 38/443 acting on CH-OH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: A61K 47/24 containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0026 Blood substitute; Oxygen transporting formulations; Plasma extender
• CPC Class Codes: C12Y 101/03013 Alcohol oxidase (1.1.3.13)

Abstract

The level of alcohol content (methanol or ethanol) in blood is reduced by contacting the blood alcohol with the enzyme alcohol oxidase. Injection, extracorporeal shunt (dialysis), and oral administration are contemplated as routes of administration. Suitable compositions for reducing the blood alcohol level are also provided.

Claims

1. A process for reducing the alcohol content of human blood comprising contacting said alcohol with the enzyme alcohol oxidase wherein said alcohol oxidase, present in an injectable solution, is injected into the human body in the amount from about 1 to about 100 mg/Kg of body weight of the recipient.

2. A process according to claim 1 wherein said alcohol is ethanol or methanol.

3. A process according to claim 1 wherein said alcohol oxidase is present in either an aqueous or isotonic saline solution.

4. A process according to claim 3 wherein said alcohol oxidase is admixed with an oxygen carrier.

5. A process according to claim 4 wherein said oxygen carrier is an aqueous emulsion of perfluorodecalin and perfluorotripropylamine.

6. A process according to claim 4 wherein said alcohol oxidase is combined with an antioxidant.

7. A process according to claim 6 wherein said antioxidant is one of ascorbic acid, ascorbyl palmitate, and monothioglycerol.

8. A process according to claim 4 wherein said alcohol oxidase is administered by intravenous injection.

9. A process according to claim 4 wherein said alcohol oxidase is administered by intramuscular injection.

10. A process according to claim 4 wherein said alcohol oxidase is administered by intraperitoneal injection.

11. A process according to claim 4 wherein niacin is present in the injection solution.

12. A process according to claim 4 wherein said alcohol oxidase is attached to either dextran or a polyethylene glycol.

13. A process according to claim 4 wherein said alcohol oxidase is in a microencapsulated form.

14. A process for reducing the alcohol content of human blood wherein said blood is contacted by renal dialysis with said alcohol oxidase, present in sterile solution, in an amount of from 1-200 mg of alcohol oxidase/Kg of body weight of the recipient.

15. A process according to claim 14 wherein said blood is contacted with said alcohol oxidase for a time sufficient to reduce the level of said alcohol in the blood stream.

16. A process for reducing the alcohol content of human blood wherein alcohol oxidase is administered orally to a recipient as a pill or tablet containing an amount of alcohol oxidase from about 1 mg to about 1 gram.

17. A process according to claim 16 wherein said alcohol oxidase is admixed with a binder.

18. A process according to claim 17 wherein said binder is one of acacia, gelatin, sucrose, methyl cellulose, alginic acid, or guar gum.

19. A process according to claim 16 wherein said alcohol oxidase is admixed with a diluent.

20. A process according to claim 19 wherein said diluent is one of lactose, starch, dibasic calcium phosphate or calcium sulfate.

21. A process according to claim 16 wherein said alcohol oxidase is admixed with a disintegrating agent.

22. A process according to claim 21 wherein said disintegrating agent is one of starch, alginic acid, microcrystalline cellulose, cross-linked providone or collodial silicates.

23. A process according to claim 16 wherein said alcohol oxidase is within an enteric coating suitable to slow down its inactivation in the stomach.

24. A process for reducing the alcohol content of human blood wherein alcohol oxidase, present in amounts from about 1-200 mg/mL of tonic, is administered to a recipient in the form of a tonic in an amount of from 1 to 50 mL.

25. A process according to claim 24 wherein niacin is admixed with said alcohol oxidase.

26. A compositions suitable for injection into the human body which comprises the enzyme alcohol oxidase present in an injectable solution in an amount sufficient to reduce the level of alcohol in human blood.

27. A composition according to claim 26 suitable for injection into the human body which comprises the enzyme alcohol oxidase present in either an aqueous or a isotonic saline solution.

28. A composition according to claim 27 further comprising an oxygen carrier.

29. A composition according to claim 28 further comprising an antioxidant.

30. A composition according to claim 29 wherein said antioxidant is one of ascorbic acid, ascorbyl palmitate, monothioglycerol or sodium bisulfite.

31. A composition according to claim 28 wherein said alcohol oxidase is attached to either dextran or polyethylene glycol.

32. A composition in tablet form suitable for oral consumption which comprises the enzyme alcohol oxidase in an amount sufficient to reduce the level of alcohol in the blood and a binder.

33. A composition according to claim 32 in the form of a tablet wherein said alcohol oxidase is present in an amount ranging from about 1 mg to about 1 g per tablet.

34. A composition according to claim 33 further comprising a diluent.

35. A composition according to claim 34 further comprising a disintegrating agent.

36. A composition according to claim 35 further comprising a lubricant.

37. A composition according to claim 36 which has an enteric coating suitable to diminish inactivation of said alcohol oxidase by the stomach.

38. A composition in the form of a tonic suitable for oral administration comprising alcohol oxidase present in an aqueous solution in an amount of about 2-200 mg per ml and sufficient to reduce the level of alcohol oxidase in human blood.

39. A composition according to claim 38 wherein said alcohol oxidase is present in an amount of from about 1-200 mg of alcohol oxidase per ml of tonic solution.