Polarization fluoroimmunoassay apparatus
First Claim
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1. Apparatus for determining the extent of an immunoreaction by means of fluorescent tagged species comprising the combination of:
- (a) an excitation light beam source for producing high intensity light for exciting fluorescent radiation in such tagged species;
(b) means for positioning a sample in the path of said excitation light beams;
(c) means for guiding the light beam to the sample while shielding the sample from the heat of the beam;
(d) means in the excitation light beam path for polarizing the excitation light beam directed toward the sample position; and
(e) means for measuring fluorescent light emitted from the sample.
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Abstract
Fluoroimmunoassay of fluorescently tagged species using a fiber optical light guiding system, and single or paired fluorescent detectors, and polarizing the activating radiation and both fluorescent radiation outputs and apparatus for carrying out such assay is disclosed.
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4 Claims
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1. Apparatus for determining the extent of an immunoreaction by means of fluorescent tagged species comprising the combination of:
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(a) an excitation light beam source for producing high intensity light for exciting fluorescent radiation in such tagged species; (b) means for positioning a sample in the path of said excitation light beams; (c) means for guiding the light beam to the sample while shielding the sample from the heat of the beam; (d) means in the excitation light beam path for polarizing the excitation light beam directed toward the sample position; and (e) means for measuring fluorescent light emitted from the sample. - View Dependent Claims (2, 3, 4)
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Specification