NMR System for the non-invasive study of phosphorus metabilism

• US 4,452,250 A
• Filed: 04/29/1982
• Issued: 06/05/1984
• Est. Priority Date: 04/29/1982
• Status: Expired due to Term

• Current Assignee: Varian Incorporated (Agilent Technologies, Inc.)
• Original Assignee: Britton Chance, John S. Leigh, Scott M. Eleff
• Inventors: Chance, Britton, Leigh, John S., Eleff, Scott M.
• Primary Examiner(s): Howell, Kyle L.
• Assistant Examiner(s): Jaworski, Francis J.
• Application Number:
  US06/373,283
• Time in Patent Office: 768 Days
• Field of Search: 128/653, 128/630, 324/309-312, 324/318, 324/322
• US Class Current: 600/410
• CPC Class Codes: G01R 33/465 applied to biological material, e.g. in vitro testing

Abstract

A nuclear magnetic resonance analytic apparatus for obtaining tissue metabolism information from a living body member. The apparatus consists of a large high-intensity electromagnet with an inner space having a uniform magnetic field, for receiving the body member. The space has a probe coil mounted therein with which the body member is engageable. For phosphorus-31 metabolite, there is provided a 24.33 MHz RF generator connected through a control logic section and a driver unit to the probe coil via a transmitter/receiver switching unit arranged to in effect switch the probe coil from a normal connection with a demodulation circuit to the driver unit responsive to a burst of RF pulse energy applied to the switching unit by the driver unit via the control logic section by command of a computer. The control logic section controls the system so as to first apply short pulses of the RF signal to the probe coil for a predetermined excitation time period, gating-off the demodulation unit. After this excitation period, the transmitter circuit is disabled, the demodulation circuit is gated-on, and the switching unit restores the normal connection of the probe coil to the demodulation unit, allowing the free induction decay signals sensed by the probe coil to be demodulated and furnished in analog form to the computer, which converts these analog signals into representations of the intensities versus frequency shift of the respective metabolite components of the tissue. Means is provided for applying a predetermined simulated free induction decay signal to the demodulation circuit for testing the apparatus.

Claims

1. An analytic apparatus for obtaining metabolism information from a living body member comprising a hollow electromagnet having an inner space for receiving the body member, said space having means providing a substantially uniform high-intensity magnetic field and containing a probe coil with which the body member is engageable when received in said space, means generating a radio-frequency signal with a carrier frequency corresponding to the nuclear magnetic resonance frequency of a metabolite in the tissue of the body member, means to repetitively apply short pulses of said radio-frequency signal to said probe coil, whereby to excite nuclear resonance responses of the metabolite, radio frequency demodulation means tuned substantially to said resonance responses, control logic means controlling the application of said radio-frequency signal pulses to said probe coil, circuit means connecting the probe coil to the demodulation means and including switch means, said switch means having transistor controlled means to greatly increase the impedance between the probe coil and the demodulation means responsive to application of said radio-frequency signal pulses, means to substantially remove said impedance after a predetermined period of application of said short pulses to the probe coil, and signal conversion means connected to the output of said demodulation means for converting the output signals of the demodulation means into a representation of the intensities versus frequency shift of the free induction decays of the respective metabolite components of the tissue.

2. The analytic apparatus of claim 1, and wherein the carrier radio frequency corresponds substantially to the nuclear magnetic resonance frequency of phosphorus-31.

3. The analytic apparatus of claim 1, and wherein the carrier frequency is approximately 24.33 MHz.

4. The analytic apparatus of claim 1, and means to at times generate a simulated predetermined free induction decay signal, and means to apply said simulated decay signal to the input of said demodulation means for testing the apparatus.

5. The analytic apparatus of claim 4, and wherein said simulated decay signal comprises an exponentially decaying 1 kHz sine wave.

6. The analytic apparatus of claim 1, and wherein said control logic means includes adjustable means to provide a predetermined time delay before the enabling of the means to apply the short pulses of said radio-frequency signal to said probe coil.

7. The analytic apparatus of claim 1, and wherein the control logic means comprises a main control logic unit connected to the output of said radio-frequency signal generating means and a driver unit connected to and controlled by the output of the main control logic unit, said main control logic unit including circuit means for connecting said radio-frequency generating means to said driver unit.

8. The analytic apparatus of claim 7, and gating means connecting said main control logic unit to said demodulation means and disabling said demodulation means when the driver unit is enabled.

9. The analytic apparatus of claim 7, and timed means to enable said demodulation means and to disable said driver means during the operation of said demodulation means.

10. The analytic apparatus of claim 7, and a computer operatively connected to said main contol logic unit, said logic unit including means to furnish a first handshake signal to said computer when said demodulation means is disabled, and means to furnish a second handshake signal to said computer at the end of a timed period of radio-frequency signal delivery to the probe coil by the driver unit.

11. The analytic apparatus of claim 7, and wherein said main control logic unit includes means to provide an adjustable time delay before transmitter radio-frequency pulses are delivered to the probe coil by the driver unit.

12. The analytic apparatus of claim 7, and a computer operatively connected to said main control logic unit, said logic unit including means to furnish a low level handshake signal to the computer shortly after the start of an operating cycle in which the driver unit is enabled, means to gate-off the demodulation means during delivery of the radio-frequency pulses to the probe coil by the driver unit, means to terminate radio-frequency pulses to the driver unit at the end of a timed delivery period, means to gate-on the demodulation means shortly after completion of said delivery period, and means to then furnish a high level handshake signal to the computer to indicate that the transmit cycle is complete and data is ready to be acquired.

13. The analytic apparatus of claim 7, and wherein said switch means has a transmitter terminal connected to the output of said driver unit, a receiver terminal connected to said demodulation means, and a probe terminal connected to said probe coil, variable impedance means connected between said probe terminal and said receiver terminal, said impedance means being relatively low in impedance during receive times, conductive circuit means connected between said transmitter terminal and said probe terminal, and means to substantially increase the impedance of said variable impedance means responsive to a burst of radio-frequency energy applied to said transmitter terminal by said driver unit and furnished to the probe coil via said conductive circuit means and probe terminal, whereby to limit the input to said demodulation means during transmit times.

14. The analytic apparatus of claim 1, and wherein said switch means has a transmitter terminal connected to said radio-frequency generating means, a receiver terminal connected to said demodulation means, and a probe terminal connected to said probe coil, variable impedance means connected between said probe terminal and said receiver terminal, said impedance means being relatively low in impedance during receive times, conductive circuit means connected between said transmitter terminal and probe terminal, and means to substantially increase the impedance of said variable impedance means responsive to a burst of radio-frequency signal energy applied to said transmitter terminal and furnished to the probe coil via said conductive circuit means and probe terminal, wherein said variable impedance means comprises a transformer with its primary connected between the probe terminal and the receiver terminal, circuit means including a conducting transistor substantially defining a short circuit across the secondary of the transformer during receive times, and negative biasing circuit means connected to the base circuit of said transistor for cutting off said transistor responsive to the application of said burst of energy to the transmitter terminal, whereby to open said short circuit and produce a high impedance condition of said transformer primary so as to substantially isolate the probe terminal from the receiver terminal and to limit the input to said demodulation means during transmit times.