Apparatus for measuring clinical emergency check items of blood

• US 4,452,682 A
• Filed: 10/24/1980
• Issued: 06/05/1984
• Est. Priority Date: 10/24/1980
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Shibata, Yasuhisa, Miyagi, Hiroyuki, Takata, Yoshinori, Hirota, Kunio, Nidaira, Kazuo, Shirato, Fusao
• Primary Examiner(s): TUNG, TA HSUNG
• Application Number:
  US06/200,264
• Time in Patent Office: 1,320 Days
• Field of Search: 204/195 B, 204/195 P, 204/195 M, 204/1 T, 204/195 R, 204/195 G, 23/253 R, 23/230 B, 128/2 E, 128/2 G
• US Class Current: 204/403.12
• CPC Class Codes: G01N 33/4915 using flow cells flow cytometry G01N15/14
• CPC Class Codes: G01N 33/492 Determining multiple analytes

Abstract

An apparatus for electrochemically measuring clinical emergency check items of blood for a short time by flowthrough system, using a small amount of whole blood as a blood sample, which comprises a blood pH/gas component sensor unit, an electrolyte component sensor unit, and/or a biochemical component sensor unit, arranged in series in this order, a sampler and these sensor units being communicated with one another through a blood sample flow passage or channel, each of the sensor units being provided with sensor electrodes and at least one reference electrode as electrochemical sensor means.

Detected values from each of the sensor units are transmitted to a computer or calculator through a current amplifier and/or a differential amplifier for a differential voltage from the voltage of the reference electrode to compute pH, pressure, or concentrations from the detected values, and numerically displayed on a display device connected to the computer.

Claims

1. An apparatus for measuring blood emergency check items by electrochemically measuring pH, gas component concentrations or pressures, and electrolyte component concentrations of whole blood by a flowthrough system, which comprises:

(1) a sampler,

(2) a blood pH/gas component sensor unit comprising a flow cell provided with a pH sensor electrode, at least one gas sensing electrode and a reference electrode.

(3) a blood electrolyte component sensor unit comprising a flow cell provided with a plurality of ion sensing electrodes and a reference electrode, the sampler and the sensor units being arranged separately from each other and in series in the order given, and

(4) a blood sample flow passage providing successive communication between the sampler, the blood pH/gas component sensor unit, and the blood electrolyte component sensor unit.

4. An apparatus according to claim 1, 2 or 3, wherein the blood pH/gas component sensor unit comprises a pH sensor electrode, a P_O.sbsb.2 sensor electrode, or dissolved oxygen sensor electrode, a P_CO.sbsb.2 sensor electrode or a bicarbonate selective sensor electrode, and a reference electrode, the sensing side of each of the electrodes is in the blood sample flow passage, while the connecting side thereof is connected to a computer through a current amplifier or a differential amplifier for a differential voltage from voltage of the reference electrode, and the computer is connected to a display device, each of resulting signals being numerically displayed on the display device as pH, oxygen concentration or pressure, bicarbonate concentration upon computation in the computer.

5. An apparatus according to claim 1 or 3, wherein the blood electrolyte component sensor unit comprises a sodium ion sensor electrode, a potassium ion sensor electrode, an ammonium ion sensor electrode and/or a calcium ion sensor electrode, a chloride ion sensor electrode, and a reference electrode, the sensing side of each of the electrodes is in the blood sample flow passage, while the connecting side thereof is connected to a computer through a differential amplifier for a differential voltage from voltage of the reference electrode, the computer is connected to a display device, each of signals being numerically displayed on the display device as a sodium ion concentration, a potassium ion concentration, an ammonium ion concentration, and/or a calcium ion concentration, or a chloride ion concentration upon computation in the computer.

7. An apparatus according to claim 1, 2 or 3, where a dialysis cell is provided between the sensor units.

8. An apparatus according to claim 1, 2 or 3, wherein sample inlets are provided between the sensor units.

2. An apparatus for measuring blood emergency check items by electrochemically measuring pH, gas component concentrations or pressures, and biochemical component concentrations of whole blood by a flowthrough system, which comprises:

(1) a sampler,

(2) a blood pH/gas component sensor unit comprising a flow cell provided with a pH sensor electrode, at least one gas sensing electrode and a reference electrode.

(3) a blood biochemical component sensor unit comprising a flow cell provided with immobilized enzyme and a reference electrode, the sampler and the sensor units being arranged separately from each other and in series in the order given, and

(4) a blood sample flow passage providing successive communication between the sampler, the blood pH/gas component sensor unit, and the blood biochemical component sensor unit.

6. An apparatus according to claim 2 or 3, wherein the blood biochemical component sensor unit with immobilized enzyme comprises electrodes with immobilized enzyme reactor and reference electrodes, the sensing side of each of the electrodes is in a blood sample flow passage, while the connecting side thereof is connected to a computer through a current amplifier or a differential amplifier for a differential voltage from the voltage of the reference electrode, the computer is connected to a display device, each of signals being numerically displayed as a biochemical component concentration upon computation in the computer.

3. An apparatus for measuring blood emergency check items by electrochemically measuring pH, gas component concentrations or pressures, electrolyte component concentrations, and biochemical component concentrations of whole blood by a flowthrough system, which comprises:

(1) a sampler,

(2) a blood pH/gas component sensor unit comprising a flow cell provided with a pH sensor electrode, at least one gas sensing electrode and a reference electrode,

(3 ) a blood electrolyte component sensor unit comprising a flow cell provided with a plurality of ion sensing electrodes and a reference electrode,

(4) a blood biochemical component sensor unit comprising a flow cell provided with immobilized enzyme and a reference electrode, the sampler and the sensor units being arranged separately from each other and in series in the order given.

(5) a blood sample flow passage providing successive communication between the sampler, the blood pH/gas component sensor unit, the blood electrolyte component sensor unit, and the blood biochemical component sensor unit.

9. An apparatus for measuring blood emergency check items by electrochemically measuring pH, gas component concentrations or pressures, electrolyte component concentrations, and biochemical component concentrations of whole blood by a flowthrough system, which comprises:

(1) a blood pH/gas component sensor unit comprising a flow cell provided with a pH sensor electrode, a P_O.sbsb.2 sensor electrode and a reference electrode, the P_O.sbsb.2 sensor electrode being connected to a current amplifier, and the pH sensor electrode and the reference electrode being connected to a differential amplifier,

(2) a blood electrolyte component sensor unit comprising a flow cell provided with a plurality of ion sensing electrodes and a reference electrode, the plurality of the ion sensing electrodes being directed to detecting voltage potential,

(3) a blood biochemical component sensor unit provided with a glucose sensor means and a urea-form nitrogen sensor means, the glucose sensor means having a reactor containing immobilized glucose oxidase and either a pair of P_O.sbsb.2 sensor electrodes provided before and after the reactor containing immobilized glucose oxidase or a P_H.sbsb.2_O.sbsb.2 sensor electrode provided after the reactor containing immobilized glucose oxidase, the urea-form nitrogen sensor means being provided with a reactor containing immobilized urease, a pair of ammonium ion sensor electrodes provided before and after the reactor containing immobilized urease, and a pair of reference electrodes, and

(4) the sensor units being arranged separately from each other and in series in the order given.

10. The apparatus according to claim 9, wherein a sample inlet is provided at the upstream side of each of the sensor units.

11. The apparatus according to claim 9, wherein the reactor containing immobilized glucose oxidase is a first reactor and the reactor containing immobilized urease is a second reactor separate from said first reactor.

12. The apparatus according to claim 9, wherein the reactor containing immobilized glucose oxidase and the reactor containing immobilized urease constitute a single reactor.