Determination of the acid-base status of blood
First Claim
1. In a method for the determination of the acid-base status of blood wherein two of the three values actual blood pH, pCO2 and base excess BE along with hemoglobin content are required, the improvement wherein the determination of the base excess BE is made through measurement of the pH value of the blood at a pCO2 -value of about 0 mm Hg.
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Abstract
In order to simplify the operation for determination of the acid-base status of blood, composed of the values for pH, pCO2 and the base excess BE, the base excess BE is determined through measurement of the pH-value at a pCO2 of about 0 mm Hg. The pH-values may be determined photometrically, in which case the indicator solution for measuring the actual blood-pH is an aqueous solution of 40 micromol/l bromthymol blue plus 0.2 g/l sodium dodecylsulfate plus 1% ethanol; and for measurement of the base excess BE is an aqueous solution of 65 micro mol/l naphtholphthalein plus 0.2 g/l sodium dodecylsulfate plus 15% dimethylsulfoxide, which are measured at a wavelength of 635 nm. If desired, one may use a single solution for measuring both actual blood-pH as well as the base excess BE, composed of 16 micromol/l bromthymol blue plus 34 micromol/l naphtholphthalein plus 0.15 g/l sodium dodecylsulfate, which is measured at 615 nm wavelength.
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5 Claims
- 1. In a method for the determination of the acid-base status of blood wherein two of the three values actual blood pH, pCO2 and base excess BE along with hemoglobin content are required, the improvement wherein the determination of the base excess BE is made through measurement of the pH value of the blood at a pCO2 -value of about 0 mm Hg.
Specification