Test circuit for use in coagulation instrument

• US 4,454,752 A
• Filed: 06/21/1982
• Issued: 06/19/1984
• Est. Priority Date: 06/21/1982
• Status: Expired due to Term

• Current Assignee: Medical Laboratory Automation Incorporated
• Original Assignee: Medical Laboratory Automation Incorporated
• Inventors: Scordato, Richard E.
• Primary Examiner(s): Swisher, S. Clement
• Assistant Examiner(s): Roskos, Joseph W.
• Application Number:
  US06/390,679
• Time in Patent Office: 729 Days
• Field of Search: 73/64.1, 73/432 SD, 73/5, 73/1 R, 356/39, 364/578, 422/73, 436/69
• US Class Current: 73/64.43
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

In a photometric coagulation instrument a synthetic clot signal is generated so that the operation of the instrument can be checked without the need to measure the actual clotting time of a control plasma sample.

Claims

1. A photometric coagulation instrument comprising light source means for directing light through a plasma sample, light sensor means responsive to the light passing through a plasma sample for generating an electrical signal that is a function of the optical density of the plasma sample as a clot forms therein, circuit means responsive to the electrical signal generated by said light sensor means for determining the instant at which the plasma sample is considered to have clotted, timer means responsive to said circuit means for indicating a time measure of the clotting time of the plasma sample, additional circuit means for producing a clot simulating signal that will cause said circuit means to respond as though a plasma sample were placed in the light path between said light source means and said light sensor means, and means for activating said additional circuit means when it is desired to obtain a time measure of clotting time without using a plasma sample and thereby check the operation of said circuit means.

2. A photometric coagulation instrument according to claim 1 wherein said clot simulating signal operates to reduce the light output of said light source means so that said light sensor means responds as though the reduced light falling thereon is the result of a clot forming in a plasma sample.

3. A photometric coagulation instrument according to claim 1 wherein said clot simulating signal operates to cause said circuit means to respond as though an optical density signal from said light sensor means were being applied thereto.

4. A photometric coagulation instrument according to claim 3 wherein said light sensor means generates a constant signal as the result of unimpeded light from said light source means falling thereon, and said clot simulating signal modifies said constant signal whereby the signal to which said circuit means responds simulates an optical density signal resulting from clot formation in a plasma sample placed between said light source means and said light sensor means.

5. A photometric coagulation instrument according to claim claim 1 or 2 or 3 or 4 wherein said additional circuit means includes timer means, and means for generating a signal having a waveshape similar to that of the optical density curve during the period of clot formation.

6. A photometric coagulation instrument according to claim 5 wherein said signal generating means generates a signal that simulates only a portion of the optical density curve during the period of clot formation.

7. A photometric coagulation instrument according to claim 6 wherein said signal generating means generates a signal having a second order waveshape.

8. A photometric coagulation instrument having means for detecting clot formation in a plasma sample and for measuring the time period between the introduction of a reagent into the plasma sample and the onset of clot formation, said instrument comprising generating means for generating a clot simulating signal, and means for switching said signal generating means into the clot formation detecting means so that the instrument will measure a time period resulting from said clot simulating signal.

9. A photometric coagulation instrument according to claim 8 wherein said generating means includes timer means, and a waveform signal generator that simulates the waveform of an optical density signal during clot formation.

10. The method of simulating the optical density signal obtained in a photometric coagulation instrument after a reagent is added to a plasma sample so that the instrument can be checked to see if it measures normal clotting time without the need to test the clotting time of a control plasma, said method comprising the steps of providing a time interval corresponding to the time period that elapses before the onset of the rapid rise in clot density of a plasma sample, and providing at the expiration of said time interval a signal having a waveform corresponding to that of the optical density signal waveform obtained during the rapid rise in the clot density of a plasma sample.

11. The method of checking the operation of the clot detection circuitry of a photometric coagulation instrument which method comprises the steps of generating in a signal generating circuit a synthetic signal that simulates the signal that results during clot formation in a reagent plasma mixture, applying the synthetic signal to the clot detection circuitry of the instrument, and having the instrument indicate the clot time for the synthetic signal.

12. The method of checking the operation of the clot detection circuitry of a photometric coagulation instrument according to claim 11 wherein the synthetic signal simulates the rise in the optical density signal occurring during clot formation, and including the step of delaying the application of the synthetic signal to the clot detection circuitry for an initial time interval.

13. The method of checking the operation of the clot detection circuitry of a photometric coagulation instrument which method comprises the steps of starting the clot timing means of the coagulation instrument, providing an initial time interval, generating a second order synthetic signal that simulates the signal that results from the rise in optical density when a clot forms in a reagent plasma mixture, applying the synthetic signal to the clot detection circuitry of the instrument after the initial time interval, and having the instrument indicate the clot time for the synthetic signal.