Agents for promoting growth of epithelial cells

• US 4,456,687 A
• Filed: 12/01/1980
• Issued: 06/26/1984
• Est. Priority Date: 11/16/1978
• Status: Expired due to Term

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Green, Howard
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/211,921
• Time in Patent Office: 1,303 Days
• Field of Search: 435/2, 435/240, 435/241
• US Class Current: 435/375
• CPC Class Codes: C12N 2501/01 Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin

Abstract

Certain agents are disclosed which promote growth of epithelial cells, including human epidermal cells. These agents are known to increase the level of cellular cyclic-AMP.

Claims

1. A method of promoting growth of human epithelial cells in culture comprising contacting said cells with an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP and cell multiplication.

2. The method of claim 1 wherein the agent capable of increasing cellular cyclic-AMP comprises cholera toxin.

4. A method of claims 1 or 2 wherein said epithelial cells comprise stratified squamous epithelium.

6. A method of claims 1 or 5 wherein said epithelial cells are grown in the presence of fibroblast cells treated to prevent their multiplication or medium conditioned by fibroblast cells.

3. In a method for serially culturing epithelial cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures:

the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture in order to stimulate cell multiplication.

5. A method of promoting multiplication of human epithelial cells in culture by increasing the level of cyclic-AMP in said cells.

7. In a method for serially culturing epithelical cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures:

the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture.

8. The improvement of claim 7 wherein said agent is selected from the group consisting of cholera toxin, methyl isobutyl xanthine and isoproterenol.

9. A method of claim 8 wherein said epithelial cells comprise stratified squamous epithelium.

10. A method of claim 9 wherein said culture contains fibroblast cells treated to prevent their multiplication, said treated cells being present at a density sufficient to allow epithelial colonies to grow.

11. A method of claim 10 wherein said culture additionally contains an effective growth-promoting amount of epidermal growth factor.

12. The method of claim 7 wherein the agent capable of increasing cellular cyclic-AMP comprises cholera toxin.

13. A method for serially culturing epithelial cells, comprising the steps of:

forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow and/or medium sufficiently conditioned by fibroblast cells to allow epithelial colonies to grow;

supplying an effective growth-promoting amount of cholera toxin capable of increasing cellular cyclic-AMP to said culture;

maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed;

harvesting said epithelial colonies; and

,

serially re-plating said epithelial cells into subcultures.