Cell-seeding procedures involving fibrous lattices
First Claim
1. A method of promoting tissue generation at a wound, comprising the following steps:
- a. creating a fibrous lattice which;
(1) has a controllable biode-gradability in the presence of body enzymes;
(2) provokes no substantial immune response or inflammation when grafted or implanted into a wound;
(3) has sufficiently high porosity, pore size and permeability to allow cell migration and reproduction therein; and
(4) has sufficiently high strength to resist tearing and collapse if grafted onto a wound;
b. securing said lattice in contact with said wound;
c. selecting cells which promote tissue growth at said wound; and
d. seeding said cells onto or into said lactice to promote generation of tissue.
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Abstract
This invention relates to the introduction of viable cells into a fibrous lattice by surgical, force-utilizing, or other manipulative techniques, all of which are referred to herein as "seeding." One embodiment comprises an autografting technique which involves intact tissue. Other embodiments which involve the distribution of an aqueous suspension of cells comprise centrifugal, spraying, pipetting and syringe emplacement. Each cell that survives the seeding process may reproduce into a colony of cells which grow until they reach confluence, thereby creating a uniform layer or volume of tissue. By proper placement of cells capable of generating such colonies, the period of recovery of a wound may be drastically reduced. In addition, research by the Applicants indicates that tissue that is regenerated using the methods of this invention to seed a lattice comprising collagen and glycosaminoglycan tends to be smoother, more uniform, less fibrotic, less scarred and more flexible than tissue that is regenerated by wound contraction or conventional autografting.
415 Citations
16 Claims
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1. A method of promoting tissue generation at a wound, comprising the following steps:
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a. creating a fibrous lattice which; (1) has a controllable biode-gradability in the presence of body enzymes; (2) provokes no substantial immune response or inflammation when grafted or implanted into a wound; (3) has sufficiently high porosity, pore size and permeability to allow cell migration and reproduction therein; and (4) has sufficiently high strength to resist tearing and collapse if grafted onto a wound; b. securing said lattice in contact with said wound; c. selecting cells which promote tissue growth at said wound; and d. seeding said cells onto or into said lactice to promote generation of tissue. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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2. A method of promoting tissue generation at a wound, comprising the following steps:
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a. creating a fibrous lattice which has morphological characteristics that substantially resemble the morphological characteristics of the intercellular proteinous matrix that normally exists within healthy tissue at the location of said wound; b. securing said lattice in contact with said wound; c. selecting cells which promote tissue growth at said wound; and d. seeding said cells onto or into said lattice to promote generation of tissue.
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Specification