Multiple-component binding assay system and method of making and using it

• US 4,459,360 A
• Filed: 10/05/1981
• Issued: 07/10/1984
• Est. Priority Date: 10/05/1981
• Status: Expired due to Term

• Current Assignee: MAST Medical Industries Limited
• Original Assignee: MAST Medical Industries Limited
• Inventors: Marinkovich, Vincent A.
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/308,935
• Time in Patent Office: 1,009 Days
• Field of Search: 424/1, 424/12, 422/56-58, 422/61, 422/68, 436/530, 436/513, 436/804, 436/808, 436/810
• US Class Current: 436/513
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A multiple component binding assay system, and related methods of making and using it, having a plurality of coated filaments mounted at their opposite ends on a support, in a predetermined spaced relationship, for simultaneously screening a liquid test sample for a plurality of components. The system is particularly suitable for screening the sample for IgE class antibodies specific for certain allergens. The filaments are preferably cotton threads, each thread binding a different allergen through a covalent bond. The support, which is preferably cut away adjacent the mid-portion of each filament to reduce background interference, is composed of a plastic or other water insoluble material, permitting inexpensive and relatively simple manufacture. After any IgE class antibodies present in the liquid sample have reacted with the corresponding allergens coated on the filaments, they are detected using second antibodies labeled with radioactive, fluorescent or enzymatic tracers. By using a high energy radioactive tracer, such as ¹²⁵ I, autoradiographic techniques with phosphor screen enhancement allow for diagnostic analysis by a densitometer or even the unaided human eye.

Claims

1. For use in the diagnostic analysis of a liquid specimen through binding assays, a diagnostic system comprising a plurality of filaments supported in a spaced relationship for simultaneous contact with said liquid specimen, at least two of said filaments being coated with binding assay components from different assays, for multiple, coincident measurement of said assays.

2. The diagnostic system of claim 1 wherein said binding assay component is an antigen.

3. The diagnostic system of claim 1 wherein said binding assay component is an allergen.

4. The diagnostic system of claim 1 wherein said filaments consist essentially of cotton.

5. The diagnostic system of claim 1 wherein said filaments are threads.

52. The diagnostic system of claim 1 wherein:

the system further includes an elongated, flat support on which the plurality of filaments are supported; and

the filaments are oriented substantially parallel to each other and perpendicular to the longitudinal axis of the support.

53. The diagnostic system of claim 52 wherein the support contacts the filaments only at their respective ends.

6. A diagnostic test kit useful in screening for the presence of multiple allergen-specific IgE class antibodies in a liquid test sample, said kit comprising a plurality of threads supported in a predetermined spaced relationship for simultaneous contact with said test sample, wherein at least two of said threads are coated with allergen compositions specific to different IgE class antibodies.

7. The kit of claim 6 wherein the threads are cotton.

8. The kit of claim 6 wherein the threads consist essentially of a polysaccharide.

9. The kit of claim 6 wherein each allergen composition is bound to a thread through a covalent bond.

10. The kit of claim 9 wherein said covalent bond is formed with a cyanogen halide.

11. The kit of claim 6 further comprising labeled antibodies reactive with said IgE for detection and measurement of IgE class antibodies bound to said thread.

12. The kit of claim 11 wherein the antibodies reactive with said IgE class antibodies are labeled with a radioactive tracer.

13. The kit of claim 12 wherein said radioactive tracer is ¹²⁵ I.

14. The kit of claim 11 wherein the antibodies reactive with said IgE class antibodies are labelled with a fluorescent tracer.

15. The kit of claim 11 wherein the antibodies reactive with said IgE class antibodies are labelled with an enzyme.

16. The kit of claim 11 wherein the antibodies reactive with said IgE class antibodies are labeled with an enzyme substrate or coenzyme.

17. A method of detecting the presence of biological agents in a liquid specimen through binding assays, said method comprising the steps of:

incubating said liquid specimen with a plurality of filaments supported in a predetermined spaced relationship, at least two of said filaments being coated with binding assay components from different assays; and

analyzing said filaments to coincidently determine which of the biological agents, interact with said binding assay component coated on each of said filaments.

18. The method of claim 17 wherein said binding assays are immunoassays.

19. The method of claim 17 wherein said binding assay components are allergens.

20. The method of claim 17 wherein said filaments are analyzed by autoradiography.

21. A method of screening for the presence of multiple allergen-specific IgE class antibodies in a liquid test sample, said method comprising the steps of:

incubating said test sample with a plurality of filaments supported on a support in a predetermined spaced relationship and coated with allergens specific to said IgE class antibodies;

removing said filaments from said test sample;

incubating said filaments with a solution containing labeled antibodies that are reactive with said IgE class antibodies;

removing said filaments from said solution; and

analyzing said filaments to determine the presence of said labeled antibodies;

wherein at least two of said filaments are coated with different allergens.

22. The method of claim 21 wherein the labeled antibodies are radioactive.

23. The method of claim 22 wherein the radioactive antibodies contain ¹²⁵ I.

24. The method of claim 22 wherein the analysis is accomplished with a gamma counter.

25. The method of claim 22 wherein the analysis of the filament for the presence of labeled antibodies is accomplished by placing the filaments proximate to a film for exposing said film by said labeled antibodies.

26. The method of claim 25 wherein one or more phosphor screens are placed adjacent said film and said filaments to increase film exposure.

27. The method of claim 25 wherein the film is read by the unaided human eye.

28. The method of claim 25 wherein the film is read by a densitometer.

29. The method of claim 28 wherein the densitometer is a scanning densitometer.

30. The method of claim 21 wherein the labeled antibodies are fluorescent.

31. The method of claim 30 wherein the analysis of the filaments for the presence of labeled antibodies is accomplished with a fluorometer.

32. The method of claim 21 wherein the labelled antibodies are labeled with an enzyme, enzyme substrate or coenzyme.

33. The method of claim 21 wherein the analysis is accomplished with a spectrophotometer.

34. The method of claim 21 wherein a portion of the support is cut away to minimize background interference.

35. The method of claim 21 wherein the plurality of filaments comprises at least about eight filaments.

36. A method of making an apparatus useful in the diagnostic analysis of liquid specimens through binding assays, said method comprising the steps of:

coating each of a plurality of filaments with components of different binding assays; and

attaching the coated filaments to a support such that they are arranged in spaced relationship.

37. The method of claim 36 wherein said binding assay is an immunoassay.

38. The method of claim 36 wherein said binding assay component is an antigen.

39. The method of claim 36 wherein said binding assay component is an allergen.

40. The method of claim 36 wherein said support consists essentially of water insoluble material.

41. The method of claim 36 further comprising the step of cutting the support and attached filaments into a plurality of elongated strips of predetermined size.

42. The method of claim 36 wherein said binding assay component is covalently bound to said filament.

43. The method of claim 36 wherein said filament is a cotton thread.

44. A method of making a miniature apparatus for use in the simultaneous determination of the presence of multiple allergen-specific IgE class antibodies in a liquid test sample, said method comprising the steps of:

coating a filament with an allergen composition specific to at least one of said IgE class antibodies; and

attaching a plurality of said filaments to a support;

wherein at least two of said filaments attached to said support are coated with different allergen compositions.

45. The method of claim 44 further comprising the step of cutting said support and attached filaments into a plurality of elongated strips of predetermined size.

46. The method of claim 44 wherein one of said allergen composition comprises a mixture of related allergens.

47. The method of claim 46 wherein said mixture is a grass mix, mugwort mix, olive mix, ragweed mix, walnut mix, alternaria mix, dematiaceae mix or penicillium mix.

48. The method of claim 44 wherein said allergen composition is a defatted and extracted allergen.

49. The method of claim 48 wherein said allergen composition is covalently bound to said filament.

50. The method of claim 49 wherein said filament is a cotton thread.

51. The method of claim 50 wherein said covalent bond is formed with cyanogen bromide.

54. A method of detecting the presence of multiple biological agents in a liquid specimen through binding assays, comprising steps of:

incubating the liquid specimen with a diagnostic device having a plurality of filaments secured to a support member, at least two of the filaments being coated with binding assay components that react with different biological agents that might be present in the specimen;

separating the liquid specimen and the diagnostic device from each other;

incubating the diagnostic device with a solution containing labeled components that will bind to any of the biological agents that might have bound to the binding assay components in the first step of incubating;

separating the diagnostic device and the solution from each other; and

positioning the diagnostic device proximate to a photographic film, to expose a pattern in the film indicative of the presence of labeled components on each of the filaments.

55. The method of claim 54 wherein:

the labeled components present in the solution used in the second step of incubating are labeled with a radioactive tracer; and

the method further includes a step of positioning a phosphor screen adjacent to the photographic film, to enhance the film exposure.

56. The method of claim 54 wherein:

the filaments are secured at least at their opposite ends to the support member in a substantially parallel and spaced apart relationship such that the step of positioning results in a pattern of spaced lines being exposed in the film, each such line having an exposure indicative of the concentration of labeled components on the corresponding filament; and

the method further includes a step of measuring the exposure of the spaced lines in the film.

57. The method of claim 54 wherein:

the biological agents detected by the method are allergen-specific IgE class antibodies;

the binding assay components coated on the filaments are allergens; and

the labeled components contained in the solution used in the second step of incubating are anti-IgE antibodies.