Ascorbate-resistant broad range glucose test composition, test device and method

• US 4,460,684 A
• Filed: 08/02/1982
• Issued: 07/17/1984
• Est. Priority Date: 08/02/1982
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Bauer, Robert
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/404,445
• Time in Patent Office: 715 Days
• Field of Search: 436/66, 436/904, 436/95, 435/14, 435/28, 435/805
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 2326/00 Chromogens for determinations of oxidoreductase enzymes
• CPC Class Codes: C12Q 2326/50 Phenols; Naphthols; Catechols
• CPC Class Codes: C12Q 2326/96 4-Amino-antipyrine
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators

Abstract

A composition, test device and method for detecting the presence and/or determining concentration of glucose in a liquid test sample are disclosed. The composition is capable of determining glucose concentration over a broad range, e.g. 0 to about 5,000 milligrams (mg) of glucose per deciliter (dl) of test sample. Moreover, the composition is resistant to interference from the presence of ascorbate in the sample. The composition comprises glucose oxidase, peroxidase and, as an indicator capable of producing a detectable response in the presence of peroxidase and H₂ O₂, a mixture of two compounds. One has the structure ##STR1## and the other has the structure ##STR2## in which R, same of different, is H or lower alkyl, R'"'"' is aryl and R" is lower alkyl.

Claims

1. A composition for detecting the presence of glucose, determining the concentration of glucose, or both, in a test sample, whereby the composition is capable of determining concentration over a range of 0 to about 5000 milligrams of glucose per deciliter of test sample, and whereby the composition is resistant to interference from the presence of ascorbate in the sample,

the composition comprising glucose oxidase, peroxidase and, as an indicator capable of producing a detectable response in the presence of peroxidase and H₂ O₂, a mixture of two compounds, one having the structure ##STR6## and the other having the structure ##STR7## in which R, same or different is H or lower alkyl, R'"'"' is aryl and R" is lower alkyl.

2. The composition of claim 1 in which the compounds are 4-aminoantipyrine and 4-methylcatechol.

3. A test device for detecting the presence and/or determining the concentration of glucose in a test sample comprising a carrier matrix incorporated with the composition of claim 1 or 2.

4. The test device of claim 3 in which the carrier matrix is paper.

5. A method for detecting the presence and/or determining the concentration of glucose in a test sample comprising contacting the sample with the composition of claim 1 or 2, and observing a detectable response.

6. A method for detecting the presence and/or determining the concentration of glucose in a test sample comprising contacting the sample with the test device of claim 3 and observing a detectable response.

7. A method for detecting the presence and/or determining the concentration of glucose in a test sample comprising contacting the sample with the test device of claim 4 and observing a detectable response.