Method for externally treating the blood

• US 4,464,166 A
• Filed: 06/30/1983
• Issued: 08/07/1984
• Est. Priority Date: 06/12/1981
• Status: Expired due to Term

• Current Assignee: Eleanor F. Bourke, Fredric A. Bourke, Richard L. Edelson, Trust Edelson
• Original Assignee: Eleanor F. Bourke, Frederic A. Bourke, Jr., Richard L. Edelson, The Edelson Trust
• Inventors: Edelson, Richard L.
• Primary Examiner(s): Lesmes, George F.
• Assistant Examiner(s): Swisher, Nancy A. B.
• Application Number:
  US06/509,807
• Time in Patent Office: 404 Days
• Field of Search: 604/4, 604/5, 604/6, 604/20, 604/28, 128/395, 422/44
• US Class Current: 604/6.08
• CPC Class Codes: A61K 41/10 Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person
• CPC Class Codes: A61M 1/3616 Batch-type treatment
• CPC Class Codes: A61M 1/3623 Means for actively controlling temperature of blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3686 by removing photoactive agents after irradiation
• CPC Class Codes: Y10S 128/03 Heart-lung

Abstract

A method and system are disclosed for externally treating human blood, with the objective of reducing the functioning lymphocyte population in the blood system of a human subject. According to the method, blood is withdrawn from the subject and passed throught an ultraviolet radiation field in the presence of from about 1 nanogram to 100 micrograms per ml of blood, of a dissolved photoactive agent capable of forming photo-adducts with lymphocytic-DNA, mobile cortisone receptors or antigen sites to thereby effect covalent bonding between the photoactive agent and the same, thereby inhibiting the metabolic processes of the lymphocytes or complexing them; and thereupon returning the irradiated blood to the subject. The withdrawn blood may be formed into an extracorporeal stream and flowed through a treatment station whereat the irradiation is effected, as for example by exposure to UV radiation; and such flow process may be conducted on a continuous basis. If desired, at least portions of the treated blood may then be separated, as for example by a continuous centrifuge, before returning the remaining diverted blood to the subject.

Claims

1. A method for reducing the functioning population of a nucleated cell in the blood supply of a human subject, comprising the steps of:

withdrawing blood from said subject, and irradiating said withdrawn blood with UV radiation in the presence of a dissolved photoactivated antibody specific for the nucleated blood cell and capable when activated by said UV radiation of forming photoadducts with the nucleated blood cell, to thereby effect chemical bonding between said photoactivated antibody and the nucleated cells, thereby creating irreversible complexes of said antibody and said nucleated blood cells; and

returning the irradiated blood to said subject.

2. A method in accordance with claim 1 wherein the nucleated blood cells are lymphocytes.

3. A method in accordance with claim 1 or 2 wherein said withdrawn blood is formed into an extracorporeal flowing stream, passed through a treatment station whereat said irradiation is effected and returned to said subject in a continuous operation.

4. A method in accordance with claim 2 including the further step of separating at least portions of said photoactivated antibody-lymphocyte complexes before returning said blood to said subject.

5. A method in accordance with claim 2 including the further step of separating at least portions of said photoactivated antibody-lymphocyte complexes before returning said blood to said subject, by passing said bloodstream through a filtration system or a continuous flow centrifuge.

6. A method in accordance of claim 5, wherein excess quantities of said photoactivated antibody are removed from the extracorporeal stream by withdrawing blood plasma at said centrifuge and replacing same with fresh photoactivated antibody-free plasma.

7. A method in accordance with claim 1 wherein said blood is irradiated with photoenergy in the UVA wavelength range, and at a radiation dose level of from about 0.1 to 100 joules/cm².

8. A method in accordance with claim 1 wherein said photoactivated antibody is dissolved in said blood by mixing same with said blood subsequent to withdrawing the blood from said subject.

9. A method in accordance with claim 1 wherein said photoactivated antibody is dissolved in said blood by administering said photoactivated antibody to said subject.

19. A method in accordance with claim 1 or 10 wherein the photoactivated chemical has an azide or diazo moiety contributing to its photoactivity.

10. A method for reducing the functioning population of an immunogenic chemical in the blood supply of a human subject, comprising the steps of:

withdrawing blood from said subject, and irradiating said withdrawn blood with UV radiation in the presence of a dissolved photoactivated antibody specific for the immunogenic chemical and capable when activated by said UV radiation of forming photoadducts with said immunogenic chemical, to thereby effect chemical bonding between said photoactivated antibody and said immunogenic chemical, thereby creating irreversible complexes of said antibody and said immunogenic chemical; and

returning the irradiated blood to said subject.

11. A method in accordance with claim 10 wherein the immunogenic chemical is an undesirable natural antibody of the human subject.

12. A method in accordance with claim 10 or 11 wherein said withdrawn blood is formed into an extracorporeal flowing stream, passed through a treatment station whereat said irradiation is effected and returned to said subject in a continuous operation.

13. A method in accordance with claim 11 including the further step of separating at least portions of said photoactivated antibody-undesirable natural antibody complexes before returning said blood to said subject.

14. A method in accordance with claim 11 including the further step of separating at least portions of said photoactivated antibody-undesirable natural antibody complexes before returning said blood to said subject, by passing said bloodstream through a filtration system or a continuous flow centrifuge.

15. A method in accordance with claim 14 wherein excess quantities of said photoactivated antibody are removed from the extracorporeal stream by withdawing blood plasma at said centrifuge and replacing same with fresh photoactivated antibody-free plasma.

16. A method in accordance with claim 10 wherein said blood is irradiated with photoenergy in the UVA wavelength range, and at a radiation dose level of from about 0.1 to 100 joules/cm².

17. A method in accordance with claim 10 wherein said photoactivated antibody is dissolved in said blood by mixing same with said blood subsequent to withdrawing the blood from said subject.

18. A method in accordance with claim 10 wherein said photoactivated antibody is dissolved in said blood by administering said photoactivated antibody to said subject.