Monoclonal antibodies to vitamin B.sub.6 and immunoassay method

• US 4,465,776 A
• Filed: 09/27/1982
• Issued: 08/14/1984
• Est. Priority Date: 09/27/1982
• Status: Expired due to Fees

• Current Assignee: Research Corporation A New York Non-Profit Corporation
• Original Assignee: Research Corporation
• Inventors: Cidlowski, John A., Viceps-Madore, Dace
• Primary Examiner(s): Padgett, Benjamin R.
• Assistant Examiner(s): Moskowitz, M.
• Application Number:
  US06/424,760
• Time in Patent Office: 687 Days
• Field of Search: 436/548, 436/505, 436/804, 436/808-810, 436/815, 436/527, 436/531, 436/504, 424/1, 424/1.1, 424/85, 424/88, 424/177, 435/172, 435/68, 435/417, 435/70, 435/240, 435/241, 435/948, 435/810, 260/112 R, 260/112.5 R
• US Class Current: 436/504
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 33/82 involving vitamins or their receptors
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/948 using viruses or cell lines
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10S 530/815 Carrier is a synthetic polymer

Abstract

A continuous hybridoma cell line which secretes recoverable quantities of monoclonal antibodies having specificity against Vitamin B₆, which antibodies are useful in a method for detecting the presence of vitamin B₆ in an animal sample.

Claims

1. A murine monoclonal antibody of the IgG class which is specific for vitamin B₆.

2. The antibody of claim 1 wherein said vitamin B₆ is selected from the group consisting of pyridoxal, pyridoxine, pyridoxamine and phosphate esters thereof.

3. The antibody of claim 1 wherein said vitamin B₆ is the biologically active form.

4. The antibody of claim 1 in detectably labeled form.

5. The antibody of claim 4 wherein said label is an enzyme label, a chromophore label or a radiolabel.

6. The antibody of claim 1 which is immobilized on a solid phase.

7. The antibody of claim 6 wherein said solid phase is plastic, glass or latex.

8. The antibody of claim 6 wherein said solid phase is a slide, a tube or a plastic strip.

9. A kit useful for the detection of vitamin B₆ in a sample which comprises:

a carrier being compartmentalized to receive one or more container means in close confinement therein,

a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B₆ ;

a second container means comprising a detectably labeled antibody which is specific for vitamin B₆.

12. The kit of claim 9 wherein said antibody in said second container is labeled with a radiolabel, an enzyme label or a chromophore.

13. The kit of claim 9 wherein said antibody in said second container means is a monoclonal antibody.

10. A kit useful for the detection of vitamin B₆ in a sample which comprises:

a carrier being compartmentalized to receive one or more container means in close confinement therein,

a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B₆ ;

a second container means comprising detectably labeled vitamin B₆.

11. The kit of any of claims 9 or 10 wherein said monoclonal antibody in said first container means is immobilized on said container means.

14. The kit of any of claims 9 or 10 which also comprises a multiplicity of container means with different amounts of vitamin B₆.

15. The kit of any of claims 9 or 10 wherein said vitamin B₆ is the biologically form thereof.

16. The kit of any of claims 9 or 10 wherein said container means is a tube.