Particle agglutination analyzing plate

• US 4,466,740 A
• Filed: 10/07/1981
• Issued: 08/21/1984
• Est. Priority Date: 10/09/1980
• Status: Expired due to Term

• Current Assignee: Olympus Optical Company Ltd. (Olympus Corporation)
• Original Assignee: Olympus Optical Company Ltd. (Olympus Corporation)
• Inventors: Kano, Tokio, Tamagawa, Akira
• Primary Examiner(s): Sikes, William L.
• Assistant Examiner(s): Koren, Matthew W.
• Application Number:
  US06/309,414
• Time in Patent Office: 1,049 Days
• Field of Search: 356/38, 356/39, 356/335, 356/244, 356/246, 350/536, 422/73, 422/102
• US Class Current: 356/246
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543

Abstract

A plate for use in an immunological analysis on the basis of agglutination reaction of particles, has formed therein a number of reaction vessels each having a conical bottom surface. The plate is formed by molding of chemically resistive acrylic resins. Each vessel has an inclined bottom surface and a plurality of concentric protrusions or depressions are formed in the bottom surface to allow formation of a stable basic layer of settled particles, which basic layer can assure the formation of clear and precise particle patterns on the bottom surface.

Claims

1. A particle agglutination analyzing plate, comprising:

a plurality of reaction vessels, each having an inclined bottom surface, formed in said plate;

a plurality of steps formed in the bottom surface of each of said reaction vessels, said steps having a dimension measured perpendicularly to the inclined bottom surface of 2 to 5 μ

m and a pitch measured in the inclined bottom surface of 5 to 200 μ

m.

2. The particle agglutination analyzing plate according to claim 1, wherein said plurality of steps are formed by a plurality of depressions each having a depth of 2 to 50 μ

m and a width in the inclined direction of 5 to 200 μ

m.

3. The particle agglutination analyzing plate according to claim 2, wherein said plurality of depressions are of sawtooth shape.

4. The particle agglutination analyzing plate according to claim 1, wherein said plurality of steps are formed by a plurality of protrusions each having a height of 2 to 50 μ

m and spaced apart from each other in the inclined direction by a distance of 5 to 200 μ

m.

5. The particle agglutination analyzing plate according to claim 1, wherein said bottom surface has a periphery and a lowest portion, wherein the depths and widths of the steps are gradually decreased from the periphery to the lowest portion of the bottom surface.

6. The particle agglutination analyzing plate according to claim 1, wherein said bottom surface has a periphery and a lowest portion, wherein the depths and widths of the steps are gradually increased from the periphery to the lowest portion of the bottom surface.

7. The particle agglutination analyzing plate according to claim 1, wherein said bottom surface has a periphery and a lowest portion, wherein angles between a horizontal plane and lines connecting successive tops of successively adjacent steps are gradually decreased from the lowest portion to the periphery of the inclined bottom surface.

8. The particle agglutination analyzing plate according to claim 7, wherein said angles near the lowest portion and the periphery are set at 25°

-45° and

15°

-30°

, respectively.

9. The particle agglutination analyzing plate according to claim 1, wherein said plate is formed of a molded, chemically resistive material.

10. The particle agglutination analyzing plate according to claim 1, wherein the plate comprises a plurality of vessels having different shapes and/or dimensions.

11. A plate according to claim 1, wherein said bottom surface has the shape of an inverted cone, and said plurality of steps are formed substantially from the lowest point of the inverted cone to the outer periphery of the inverted cone, and are arranged regularly and concentrically about the lowest point.

12. The particle agglutination analyzing plate according to claim, 11 wherein said lowest point of the inverted bottom surface of the vessel is flattened.

13. A plate according to claim 1, wherein said bottom surface has the shape of an inverted cone, and said plurality of steps are arranged concentrically and intermittently about the lowest point of the inverted cone.

14. A plate according to claim 1, wherein said plurality of steps are formed by a single spiral depression.

15. A plate according to claim 1, wherein said plurality of steps are formed by a single spiral protrusion.