Cell culture control
First Claim
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1. A cell culture process comprising(a) contacting cells with liquid culture medium under growth conditions to allow said cells to metabolize and grow,(b) adding a given amount of an additive to said medium which additive is toxic for the culture above a certain concentration, and determining the change of metabolic activity of said cells occurring after said adding,(c) repeating step b a plurality of times but each time only if at least a minimum change of metabolic activity was determined in the previous step b.
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Abstract
In a cell culture process, the addition of an additive is only carried out after it has been determined that the previous addition of additive has caused at least a minimum change in metabolic activity of the cells.
19 Citations
16 Claims
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1. A cell culture process comprising
(a) contacting cells with liquid culture medium under growth conditions to allow said cells to metabolize and grow, (b) adding a given amount of an additive to said medium which additive is toxic for the culture above a certain concentration, and determining the change of metabolic activity of said cells occurring after said adding, (c) repeating step b a plurality of times but each time only if at least a minimum change of metabolic activity was determined in the previous step b.
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11. An apparatus for growing a cell culture in a medium comprising
(a) a housing wherein said cell culture in said medium can be subjected to growth conditions, (b) a sensor operatively connected to said medium in said housing, (c) said sensor being capable of determining the metabolic activity of said cell culture, (d) operatively connected to said sensor a controller generating a manipulating signal if, following the previous manipulating signal, the sensor transmitted at least a minimum change of metabolic activity, (e) operatively connected to said controller and for receiving said manipulating signal, means for adding a given quantity of additive to said medium in said housing responsive to said manipulating signal.
Specification