Immunoglobulin half-molecules and process for producing hybrid antibodies
First Claim
1. A substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provides a binding site for a first antigen and the second of said half-molecules provides a chemically different binding site for the first or a second antigen;
- and wherein said half-molecules are bonded to each other through disulfide linkage.
13 Assignments
0 Petitions
Accused Products
Abstract
A process for preparing covalent hybrid antibodies is provided. These antibodies are obtained by the ordered covalent hybridization of intact immunoglobulin molecules. The generation of half-molecules from different IgG antibodies by reductive or S-sulfonation selective cleavage is followed by the ordered association of the different half-molecules. The pure covalent hybrid antibodies as new substances are also provided along with IgG half-molecules.
-
Citations
12 Claims
-
1. A substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provides a binding site for a first antigen and the second of said half-molecules provides a chemically different binding site for the first or a second antigen;
- and wherein said half-molecules are bonded to each other through disulfide linkage.
- View Dependent Claims (2, 3, 4, 5)
-
6. A process for preparing covalent hybrid antibodies comprising the steps of:
-
(A) selectively cleaving a first immunoglobulin molecule which is an antibody to a first antigen into its heavy chain-light chain half-molecules by sulfitolysis of the inter-heavy chain disulfide linkage to produce S-sulfonated half-molecules; (B) selectively cleaving a second immunoglobulin molecule which is an antibody to the first or a second antigen into its heavy chain-light chain half-molecules by reduction of the inter-heavy chain disulfide linkage to produce reduced half-molecules; and (C) combining the S-sulfonated half-molecules from step (A) with the reduced half-molecules from step (B). - View Dependent Claims (7, 8, 9, 10, 11, 12)
-
Specification