High-speed centrifugal filter assembly and method
First Claim
1. A filter assembly for use with high-speed centrifugation apparatus comprising:
- a filter body adapted to be disposed within a filter holder in a centrifugal rotor, said filter body having an outer end of generally rounded configuration, with a radius of curvature greater than that of said filter holder and defining an interior chamber;
filter plate means disposed within said interior chamber, said filter plate means defining a plurality of fluid flow paths;
a filter forming member disposed within said interior chamber of said filter body and adjacent said filter plate means, said filter forming member having a crenelated periphery for contact with a filter membrane, to form said membrane to a crenelated configuration.
1 Assignment
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Accused Products
Abstract
The filter assembly for swinging bucket type centrifuges includes a cylindrical filter body disposed within a filter body holder, both having hemispherical outer ends. The diameter of curvature of the filter body is smaller than the diameter of curvature of the holder allowing the contact surface between the holder and the body to be in the shape of a ring; centrifugal forces thus result in compression of the filter body thereby preventing cracking. The filter'"'"'s crenelated shape is provided through use of a plunger to apply force to the filter against the crenelated filter membrane forming member. The filter assembly also may have a chamber and a meniscus equalizing pore around the filter holder. The chamber and appropriately positioned pore prevent fluid from rising during centrifugation and contaminating the particulate. A method for installation includes providing a filter body with an open inner end and a rounded outer end, placing a filter plate in one recess therein and a filter membrane in the other recess therein and applying force to provide the membrane with a crenelated shape. Another method includes the additional step of positioning an insert within the body to form a fluid retaining cavity and a meniscus equalizing pore.
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Citations
28 Claims
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1. A filter assembly for use with high-speed centrifugation apparatus comprising:
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a filter body adapted to be disposed within a filter holder in a centrifugal rotor, said filter body having an outer end of generally rounded configuration, with a radius of curvature greater than that of said filter holder and defining an interior chamber; filter plate means disposed within said interior chamber, said filter plate means defining a plurality of fluid flow paths; a filter forming member disposed within said interior chamber of said filter body and adjacent said filter plate means, said filter forming member having a crenelated periphery for contact with a filter membrane, to form said membrane to a crenelated configuration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A high speed filter assembly for installation in a filter holder of a high speed centrifuge comprising:
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a filter body defining a hollow interior and having an outer end fittable into said filter holder; a filter insert member disposed within said hollow interior of said filter body and defining an interior chamber, said filter insert member having an outer end of generally rounded configuration, said filter insert member including means for contacting the inner walls of said filter body, said filter body and said filter insert forming an anti-capillarity chamber therebetween, said filter insert defining a meniscus equalizing pore connecting said interior chamber of said filter insert member and of said anti-capillarity chamber, said meniscus equalizing pore being positioned to equalize the level of fluid between said interior chamber of said filter insert member and said anti-capillarity chamber; and filter plate means disposed in contact with said filter insert member and defining a plurality of perforations for passage therethrough of fluid. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for providing a filter assembly for use in high-speed centrifugation wherein said filter assembly is placed within a filter holder rotatable at high rotational velocities comprising the steps of:
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(a) providing a filter body having a hollow interior and fittable into said filter holder; (b) positioning a filter insert member within said filter body to form a fluid retaining cavity and a meniscus equalizing pore connected thereto; (c) disposing a filter plate member in contact with said filter insert member; (d) disposing a filter membrane upon the open end of said filter body; and (e) forcibly thrusting said filter membrane into said filter body against said filter plate member, whereby said filter membrane assumes a folded form whereby it has crenelated sides and a substantially flat bottom in contact with said filter plate member and said crenelated sides are in contact with the wall of the filter body. - View Dependent Claims (21, 22)
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23. A filter assembly for high-speed centrifugation comprising:
a filter body having a peripheral wall, an outer end of generally hemispherical configuration, and an open inner end, said hemispherical outer end having a radius larger than that of a filter holder into which said filter body is placed for centrifugation, said filter body having a generally hollow interior and including a first interior shoulder extending from the wall of said filter body and including a second interior shoulder extending from the wall of said filter body and spaced from said first interior shoulder, the latter being closer to said hemispherical outer end of said filter body than the former, said filter body further including an interior side wall disposed between said first shoulder and said second shoulder to form a first recess, said second shoulder and the wall of said filter body forming a second recess, the diameter of said first shoulder being smaller than the diameter of said second shoulder, and the diameter of said first recess being smaller than the diameter of said second recess. - View Dependent Claims (24, 25, 26, 27, 28)
Specification