Analysis film and a method of analysis using the same
First Claim
1. An analysis film for analyzing an analyte in an aqueous medium based on a competitive reaction in the presence of a fixed quantity of a protein which specifically binds with said analyte and a fixed quantity of a labeled analyte which is also reactive with said protein,said analysis film comprising a reaction layer having a void volume of 50 to 90% and a thickness of 200 μ
- m to 1 mm which provides a liquid retention amount of at least 100 μ
l/cm2 which is necessary for the analysis, wherein said reaction layer;
(1) comprises a porous material,(2) contains a protein capable of specifically binding commonly with (a) said labeled analyte or its labeled analogue both being capable of modulating a signal produced by enzyme reaction as a result of a specific protein-binding reaction and with (b) said analyte, and,(3) does not substantially contain a complex of said analyte, or its analogue or said labeled analyte or its labeled analogue, with said protein.
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Accused Products
Abstract
An analysis film comprises a reagent layer composed of a porous material which contains an antibody but does not substantially contain a complex of an analyte or a labelled antigen with the antibody. In the analysis film, reagents for enzyme immune reaction of homogenous type are incorporated so that an analyte is analyzed without requiring B/F separation. An analysis method for various analytes using the same provides high sensitivity, high accuracy as well as good reproducibility and is simple and rapid.
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Citations
19 Claims
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1. An analysis film for analyzing an analyte in an aqueous medium based on a competitive reaction in the presence of a fixed quantity of a protein which specifically binds with said analyte and a fixed quantity of a labeled analyte which is also reactive with said protein,
said analysis film comprising a reaction layer having a void volume of 50 to 90% and a thickness of 200 μ - m to 1 mm which provides a liquid retention amount of at least 100 μ
l/cm2 which is necessary for the analysis, wherein said reaction layer;(1) comprises a porous material, (2) contains a protein capable of specifically binding commonly with (a) said labeled analyte or its labeled analogue both being capable of modulating a signal produced by enzyme reaction as a result of a specific protein-binding reaction and with (b) said analyte, and, (3) does not substantially contain a complex of said analyte, or its analogue or said labeled analyte or its labeled analogue, with said protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 19)
- m to 1 mm which provides a liquid retention amount of at least 100 μ
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10. In a method for analyzing analyte in an aqueous medium which comprises competitively reacting (a) said analyte in an aqueous medium, (b) a fixed quantity of a protein which specifically binds said analyte, and (c) a fixed quantity of a labeled analyte component, which is reactive with said protein, the method which comprises:
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(I) contacting an analysis film with a sample of the analyte-containing aqueous medium said analysis film comprising a reaction layer having a void volume of 50 to 90% and a thickness of 200 μ
m to 1 mm which provides a liquid retention amount of at least 100 μ
l/cm2 which is necessary for the analysis, wherein said reaction layer;(1) comprises a porous material, (2) contains a protein capable of specifically binding commonly with said labeled analyte component being capable of modulating a signal produced by enzyme reaction as a result of a specific protein-binding reaction and with said analyte, but, (3) does not contain a complex of said analyte, an analogue thereof or said labeled analogue thereof with said proein, (II) competitively reacting said analyte with said protein in said reaction layer in the presence of said labeled analyte component capable of modulating a detectable signal as a result of a specific protein-binding reaction, said detectable signal being produced by enzyme reaction, and, (III) directly measuring said modulated signal by said competitive reaction. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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Specification