Hyperproducing cellulase microorganism

• US 4,472,504 A
• Filed: 03/28/1983
• Issued: 09/18/1984
• Est. Priority Date: 03/28/1983
• Status: Expired due to Term

• Current Assignee: United States Of America As Represented By The Secretary Of The Army (Government of the United States of America)
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Gallo, Benedict J.
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US06/479,221
• Time in Patent Office: 540 Days
• Field of Search: 435/209, 435/254, 435/945, 435/252
• US Class Current: 435/209
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12N 9/2445 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: C12R 2001/885 Trichoderma
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 302/01021 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: Y10S 435/945 Trichoderma

Abstract

A process and a microorganism for synthesizing cellulase enzymes and solu proteins are described. The microorganism is a mutant strain of an Ascomycete fungus capable of the synthesizing cellulases and soluble proteins in the presence of a growth medium containing cellulose, cellulose enzymic hydrolysate sugars or lactose. The mutant strain is identified as T. reesei MCG80.

Claims

1. A biologically pure culture of T. reesei strain MCG80.

2. A biologically pure culture according to claim 1 wherein said culture has the capability to synthesize cellulase enzymes.

3. A biologically pure culture according to claim 2 wherein the synthesis of the cellulase enzymes by the organism is induced by cellulose, cellulose enzymic hydrolysate sugars or lactose.

4. A process for producing enzymes capable of catalyzing the hydrolysis of cellulose comprising:

(a) inoculating a suitable sterile growth medium with T. reesei strain MCG80, said growth medium containing an inducer of cellulase synthesis, and

(b) incubating the inoculated growth medium under conditions which will permit the growth of MCG80.

5. A process according to claim 4 wherein the inducer of cellulase synthesis is cellulose.

6. A process according to claim 5 wherein the pH is maintained at 3.75 for the first 48 hours of incubation and then at 3.5 for the remainder of the incubation.

7. A process according to claim 4 wherein the inducer of cellulase synthesis is lactose.

8. A process according to claim 7 wherein the pH is maintained at 3.5 during the incubation.

9. A process for producing soluble protein from cellulose, cellulose enzymic hydrolysate sugar or lactose substrate which comprises:

(a) inoculating sterile growth medium containing a substrate selected from the group consisting of cellulose, cellulose enzymic hydrolysate sugar and lactose with T. reesei strain MCG80, and

(b) incubating the inoculated growth under conditions which will permit the growth of strain MCG80.

10. A process according to claim 9 wherein the substrate is cellulose and wherein the pH is maintained at 3.75 for the first 48 hours of incubation and at 3.5 for the remainder of the incubation.

11. A process according to claim 9 wherein the substrate is lactose and wherein the pH is maintained at 3.5 during incubation.