Process for saccharification of rootstocks and subsequent alcohol fermentation

• US 4,474,883 A
• Filed: 02/18/1982
• Issued: 10/02/1984
• Est. Priority Date: 03/14/1981
• Status: Expired due to Fees

• Current Assignee: Hankyu Kyoei Bussan Co., Ltd., Mitsui Engineering & Shipbuilding Company Limited (Mitsui E&S Holdings Co., Ltd.), UEDA Chemical Industrial Company Limited
• Original Assignee: Hankyu Kyoei Bussan Co., Ltd., Mitsui Engineering & Shipbuilding Company Limited (Mitsui E&S Holdings Co., Ltd.), UEDA Chemical Industrial Company Limited
• Inventors: Yamamoto, Takehiko, Matsumura, Yoshikazu, Ito, Shozo, Kakutani, Kazuo, Nojiri, Michihiko
• Primary Examiner(s): LANDER, FERRIS
• Application Number:
  US06/349,859
• Time in Patent Office: 957 Days
• Field of Search: 435/99, 435/161-163
• US Class Current: 435/162
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

For saving the heat energy required for alcohol production, processes are provided for subjecting rootstocks (Rhizomes) to enzymatic hydrolysis without conventional cooking with steam in order to obtain a high concentration saccharified material which is then subjected to alcohol fermentation.

The first process comprises dipping raw rootstocks in a dilute acid for the purpose of sterilization, then crushing the sterilized rootstocks, macerating the crushed rootstocks, dextrinizing the macerated material by the action of bacterial α-amylase and adding to the dextrinized material glucoamylase and yeast for alcohol fermentation to effect saccharification thereof and subsequent alcohol fermentation.

The second process comprises sterilizing rootstocks with a dilute acid and then crushing it, followed by adding blended maceration enzymes to effect maceration amd saccharification with subsequent alcohol fermentation by yeast.

Claims

1. A process for saccharification of rootstocks and subsequent alcohol fermentation, which process comprises:

a step of immersing rootstocks in a dilute acid solution to sterilize them;

a step of crushing the resulting sterilized rootstocks;

a step of macerating the crushed rootstocks through the action of macerating enzymes composed mainly of a polygalacturonase;

a step of forming a slurry comprising liquid and starch derived solely from the product resulting from the action of said macerating enzymes on the crushed rootstocks;

a step of adding a bacterial α

-amylase to the resulting slurry to dextrinize the starch contained therein; and

a step of adding to the resulting dextrinized starch, glucoamylase as a saccharifying enzyme, and yeast for alcohol fermentation to effect saccharification and alcohol fermentation.

2. A process according to claim 1 wherein said rootstocks are at least one selected from the group consisting of white potatoes, sweet potatoes and cassava roots.

3. A process according to claim 1 wherein said polygalacturonase is pectin endopolygalacturonase.

4. A process according to claim 3 wherein said maceration enzymes contain small amounts of carboxymethylcellulase, arabinoxylanase and arabinogalactanase, in addition to said pectin endopolygalacturonase.

5. A process according to claim 1 wherein said dextrinizing step by a bacterial α

-amylase is carried out at temperature of 80°

C. to 90°

C. and at pH of 5 or higher.

6. A process according to claim 1 wherein said maceration step by said maceration enzymes is carried out at temperature of 20°

C. to 45°

C. and at pH of 3.5 to 5.0.

7. A process according to claim 1 wherein said dilute acid is an aqueous solution of at least one compound selected from the group consisting of hydrochloric acid, sulfuric acid, and nitric acid.

8. A process according to claim 7, wherein the concentration of the acid is in the range of 0.02 to 0.08 normality.

9. A process according to claim 1 wherein a portion of the liquid in the slurry is removed before the dextrinizing step.

10. A process according to claim 9, wherein said rootstocks as raw material are white potatoes.

11. A process according to claim 1, wherein said dilute acid is an aqueous solution of acetic acid.

12. A process according to claim 11, wherein the concentration of the acid is in the range of 0.04 to 0.10 normality.

13. A process according to claim 1, wherein the rootstock is at least one of white potatoes and cassava root and the maceration enzymes are added in the ratio of 5 to 200 units per 100 g. of rootstocks.

14. A process according to claim 1, wherein the rootstock is sweet potatoes and the maceration enzymes are added in the ratio of 50 to 1,000 units per 100 g. of rootstocks.

15. A process according to claim 1, wherein the maceration step is conducted for 0.5 to 1 hour and the dextrinization step is conducted for about 1 to 15 minutes.

16. A process according to claim 1, wherein 4 to 16 units per gram of starch of bacterial α

-amylase is added.

17. A process according to claim 1, wherein the saccharification and alcohol fermentation step is conducted at 25°

C. and at a pH of 4.5.

18. A process according to claim 1, wherein 2 to 10 units per gram of starch of the saccharifying enzyme are added.

19. A process according to claim 1, wherein small amounts of ammonium sulfate, potassium dihydrogen phosphate, calcium chloride and magnesium sulfate are added as nutrients for the yeast.

21. A process according to claims 1 or 20, wherein the pH of the dilute acid solution is in the range of 1.6 to 2.8.

22. A process according to claims 1 or 20, wherein the rootstocks are immersed in the dilute acid solution for a period of approximately 4 to 6 hours.

23. A process according to claims 1 to 10, wherein the rootstocks are crushed into particles having a size in the range of 8 to 20 meshes.

20. A batch process for saccharification of rootstocks and subsequent alcohol fermentation, which process comprises:

a step of immersing rootstocks in a dilute acid solution to sterilize them;

a step of crushing the resulting sterilized rootstocks;

a step of macerating the crushed rootstocks through the action of blended enzyme agents comprising cellulase, hemicellulase, pectin endopolygalacturonase, glucoamylase and acidically active α

-amylase;

a step of forming a slurry comprising liquid and starch derived solely from the product resulting from the action of said blended enzymes on the crushed rootstocks;

a step of adding yeast for alcohol fermentation to the slurry to effect alcohol fermentation.

24. A process according to claim 20 wherein said rootstocks are selected from the group consisting of sweet potatoes and casava roots.

25. A process according to claim 20 wherein said blended enzyme agents are composed mainly of maceration enzymes and starch saccharifying amylase.

26. A process according to claim 20 wherein said blended enzyme agents contain per kg of rootstocks, 300 units or more of carboxymethylcellulase as said cellulase, 100 units or more of arabinoxylanase as said hemicellulase, as 100 units or more of said arabinogalactanase, 400 units or more of said pectin endopolygaracturonase in terms of a pectin viscosity-lowering activity, 1,500 units or more of said glucoamylase in terms of a starch-saccharifying activity, and 20 units or more of said acidically active α

-amylase in terms of a starch-gelatinizing activity determined by the iodometric method.

27. A process according to claim 20 wherein said maceration and saccharification of root-stocks by the action of said blended enzyme agents are carried out at temperature of 20°

to about 45°

C. and at pH of 3.5 to 5.0.

28. A process according to claim 27, wherein the temperature is 20°

to 35°

C.

29. A process according to claim 20 wherein said alcohol fermentation step is carried out at the same time with said maceration and saccharification step.

30. A process according to claim 20 wherein said blended enzyme agents are enzymes produced by fungi.

31. A process according to claim 30, wherein said fungi are selected from the group consisting of genus Aspergillus and genus Rhizopus.

32. A process according to claim 31, wherein the fungus is Aspergillus niger.

33. A batch for saccharification of rootstocks and subsequent alcohol fermentation comprising:

(a) immersing the rootstocks in a dilute acid to sterilize them;

(b) crushing the sterilized rootstocks from (a);

(c) macerating the crushed rootstocks from (b) through the action of macerating enzymes;

(d) forming a slurry comprising liquid and starch derived solely from the product resulting from the action of said macerating enzymes on the crushed rootstocks;

(e) dextrinizing the starch from (d) through the action of dextrinizing enzymes;

(f) saccharifying and fermenting the dextrinized starch from (d) through the action of a saccharifying enzyme and an alcohol fermentation yeast to effect saccharification and alcohol fermentation.

34. A process for the saccharification of white potatoes and subsequent alcohol fermentation comprising:

(a) immersing the white potatoes in a dilute acid to sterilize them;

(b) crushing the white potatoes from (a);

(c) macerating the crushed white potatoes through the action of macerating enzymes composed mainly of a polygalacturonase;

(d) a step of forming a slurry comprising liquid and starch derived solely from the product resulting from the action of said macerating enzymes on the crushed rootstocks;

(e) removing a portion of the liquid produced in (c);

(f) dextrinizing the starch from (c) through the action of bacterial α

-amylase;

(g) saccharifying and fermenting the dextrinized starch from (f) through the action of glucoamylase and an alcohol fermentation yeast to effect saccharification and alcohol fermentation respectively.