Sampling apparatus

• US 4,475,411 A
• Filed: 03/18/1982
• Issued: 10/09/1984
• Est. Priority Date: 03/20/1981
• Status: Expired due to Term

• Current Assignee: Coulter International Corp. (Danaher Corporation)
• Original Assignee: Coulter Electronics Ltd (Danaher Corporation)
• Inventors: Wellerfors, Hans
• Primary Examiner(s): Goldberg, Gerald
• Assistant Examiner(s): Chapman, Jr., John E.
• Application Number:
  US06/359,565
• Time in Patent Office: 936 Days
• Field of Search: 422/63, 422/64, 422/67, 422/104, 422/100, 422/72, 73/864.24, 73/864.22, 73/864.21
• US Class Current: 73/864.24
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/00762 magnetic code
• CPC Class Codes: G01N 2035/0417 forming an endless chain in a vertical plane
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums

Abstract

Apparatus for taking a sample of liquid from each of a plurality of containers. Blood or similar settling liquids requiring continuous mixing are sampled for subsequent analysis from test tubes closed by rubber or plastic plugs. The test tubes are mounted along the periphery of a rotatable cassette, which is drivingly connected to a motor via gearing, a clutch and a brake, in such a manner, that the cassette rotates in order to maintain the liquids in the test tubes thoroughly mixed. The cassette is capable of stopping instantaneously when the clutch is disengaged. The cassette is locked in accurate position in front of a sampling needle by operation of the brake. The brake operates when a signal from an analysis instrument orders an electronic counter to determine the identity of the test tube to be selected and the cassette is stopped. Immediately, a pneumatic cylinder is operated to lower an arm fixing the selected test tube. A second pneumatic cylinder also is operated to lower the sampling needle to perforate the closure. The sampling needle is lowered to a definite level and, after having arrived at this position, effects a signal to the analysis instrument. Responsive to said signal, a definite liquid amount is drawn through the sampling needle, the needle withdrawn from the tube, the arm lifted, the brake disengaged, the clutch engaged and the cassette resumes its rotation.

Claims

1. Apparatus for taking a sample of liquid from each of a plurality of sealed containers each having pierceable closures, comprising a carrier, container receiving means in said carrier for receiving and holding a plurality of said sealed containers, means on the apparatus for supporting said carrier for rotation about the central axis thereof in a plane generally normal thereto and drive means for said carrier to cause the containers held thereby to follow a defined path to a sampling station disposed along the path and each container being inverted at least once during its movement along said path with movement of the carrier whereby to cause agitation of liquid in the containers, sampling means disposed at the sampling station, means for arresting a container at the sampling station with respect to said sampling means, and means for actuating said sampling means to effect withdrawal of a sample from the container, said sampling means including piercing needle means arranged at said sampling station for selective operation to pierce the closure of the sealed container when the container is stopped at the sampling station whereby to advance said needle means into the container and means for holding the container during withdrawal of sample therefrom.

2. Apparatus as claimed in claim 1, wherein said carrier is circular in configuration, said container receiving means being spaced circumferentially, said carrier being moved generally in a rotational plane which is at a substantial angle relative to the horizontal plane.

3. Apparatus as claimed in claim 2, wherein the said angle is not less than 30°

.

4. Apparatus as claimed in claims 2 or 3, wherein the carrier is capable of receiving elongate containers each having an access end outwardly facing relative to the periphery of said carrier, said containers located approximately in a common plane, said carrier being rotatable to cause the containers therein to follow a defined path of movement which lies in a plane taken generally normal to the axis of rotation of the carrier.

5. Apparatus as claimed in claims 1 or 2 wherein the containers have an elongate configuration, said containers being located each angularly directed relative to the plane in which said carrier rotates, the angle being no greater than about 45°

.

6. Apparatus for mixing and sampling for subsequent analysis blood or similar settling liquids requiring continuous mixing in test tubes sealingly closed by pierceable resilient plugs comprising:

a rotatable cassete;

means for holding and retaining the test tubes to dispose said test tubes axially normal to the rotational axis of said cassette with the plugs facing outwardly thereof along the periphery of said rotatable cassette;

drive means coupled to said cassette for rotating said cassette about its axis;

said drive means including motor, a clutch and a brake, rotation of said cassette causing the liquids in the test tubes to be thoroughly mixed, said clutch being operable to separate the drive means from the driven cassette and said brake being applied to stop the rotation of the cassette at a selected position and to lock said cassette at said position;

sampling needle means including a sampling needle at a sampling location;

means for selecting one of said test tubes and generating a stop signal, said drive means being responsive to said stop signal to stop said cassette;

means for holding the selected test tube at the sampling location during sampling;

means for advancing said sampling needle through said plug to enter said liquid sufficiently to enable withdrawal of a definitive amount of liquid;

means generating and directing a liquid withdrawal signal operative to effect withdrawal of the definitive amount of liquid and to retract the sampling needle from the plug, release the brake, reengage the clutch and resume rotation of the cassette.

7. Apparatus as claimed in claim 6, wherein the cassette has a cylindrical configuration including an outer circumferential surface which is parallel with the axis of rotation of the cassette, said mounting and retaining means being formed radially outwardly extending relative the axis of rotation and within said cassette for receiving therein test tubes mounted radially relative to the said axis of rotation, said test tubes having the plugs accessible and outwardly extending relative to said circumferential surface.

8. Apparatus as claimed in claim 6, wherein the cassette has a circular configuration including a circumferential surface, said circumferential surface including a portion at least partially beveled at least at an angle of about 45°

and said mounting means for said test tubes formed in said cassette and opening to said partially beveled surface at an angle of about 45°

relative to the axis of rotation of said cassette.

9. Apparatus as claimed in claim 6, wherein the cassette is located in a plane which deviates from the horizontal plane by an angle not less than 30°

.

10. Apparatus as claimed in claim 6, wherein the cassette is removably replaceably mounted to facilitate exchange of one cassette for another like cassette.

11. Apparatus as claimed in claim 6, wherein the clutch and the brake are of an electromagnetic type.

12. Apparatus as claimed in claim 6, wherein the sampling needle comprises at least two tubes, one tube for receiving the sample liquid, and one tube capable of allowing air to enter the test tube in order to prevent vacuum from arising in the test tube.