Test piece for quantitative analysis of substances in body fluids

• US 4,476,222 A
• Filed: 09/27/1982
• Issued: 10/09/1984
• Est. Priority Date: 08/02/1982
• Status: Expired due to Term

• Current Assignee: Eiken Kagaku Kabushiki Kaisha
• Original Assignee: Eiken Kagaku Kabushiki Kaisha
• Inventors: Wada, Hiroshi, Kosaka, Yuzo, Ohtani, Mikio
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/423,745
• Time in Patent Office: 743 Days
• Field of Search: 422/56, 422/57, 435/14, 435/26, 435/805, 435/25, 435/28
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

A testing piece for quantitative analysis of a substance in a body fluid, particularly in blood, said testing piece comprising an absorbent carrier containing in it reagents for measuring the substance and coated with a mixture of polymethylmethacrylate and polyvinyl formal. This testing piece enables the efficient and swift quantitative analysis of the substance in body fluid, especially in blood, in a short time without preliminary separating the serum from the blood.

Claims

1. A test piece for quantitative analysis of a substance in body fluid, said test piece comprising:

an absorbent carrier, reagents for measuring the substance, said reagents being contained in said absorbent carrier, and a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal.

2. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1, wherein the weight ratio of polymethylmethacrylate to polyvinyl formal in the coating is between 9:

1 and 1;

9.

3. A test piece for quantitative analysis of a substance in body fluid claimed in claim 2, wherein said weight ratio is around 1:

1.

4. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1 wherein the absorbent carrier is a member selected from the group consisting of filter paper, cotton, wood piece, synthetic fiber paper, glass fiber, and porous plastics.

5. A test piece for quantitative analysis of a substance in body fluid claimed in claim 4, wherein the absorbent carrier is filter paper.

6. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1 wherein a surface of said test piece is carried by a plastic sheet.

7. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1 wherein said reagent comprises glucose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

8. A test piece for quantitative analysis of a substance in body fluid claimed in claim 4, wherein said reagent comprises glucose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

9. A test piece for quantitative analysis of a substance in body fluid claimed in claim 6, wherein said reagent comprises glucose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

10. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1 wherein said reagent comprises galactose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

11. A test piece for quantitative analysis of a substance in body fluid claimed in claim 4, wherein said reagent comprises galactose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

12. A test piece for quantitative analysis of a substance in body fluid claimed in claim 6, wherein said reagent comprises galactose oxidase, a substance having a peroxidase activity, and an oxidizable indicator.

13. A test piece for quantitative analysis of a substance in body fluid claimed in claim 1 wherein said reagent comprises alcohol dehydrogenase, nicotine ademine dinucleotide diaphorase and a tetrazolium salt.

14. A test piece for quantitative analysis of a substance in body fluid claimed in claim 4, wherein said reagent comprises alcohol dehydrogenase, nicotine ademine dinucleotide, diaphorase, and a tetrazolium salt.

15. A test piece for quantitative analysis of a substance in body fluid claimed in claim 6, wherein said reagent comprises alcohol dehydrogenase, nicotine ademine dinucleotide, diaphorase and a tetrazolium salt.

16. A test piece for quantitative analysis of a substance in body fluid, comprising:

A. an absorbent carrier; and

B. a reagent in said absorbent carrier, said reagent being glucose oxidase, a substance having a peroxidase activity, and an oxidizable indicator; and

C. a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal, wherein the weight ratio of polymethylmethacrylate to polyvinyl formal is between 9;

1 and 1;

9.

17. A test piece for quantitative analysis of a substance in body fluid, comprising:

A. an absorbent carrier; and

B. a reagent in said absorbent carrier, said reagent being galactose oxidase, a substance having a peroxidase activity, and an oxidizable indicator; and

C. a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal, wherein the weight ratio of polymethylmethacrylate to polyvinyl formal is between 9;

1 and 1;

9.

18. A test piece for quantitative analysis of a substance in body fluid, comprising:

A. an absorbent carrier; and

B. a reagent in said absorbent carrier, said reagent being alcohol dehydrogenase, nicotine ademine dinucleotide diaphorase and a tetrazolium salt; and

C. a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal, wherein the weight ratio of polymethylmethacrylate to polyvinyl formal is between 9;

1 to 1;

9.

19. A test piece for quantitative analysis of glucose in body fluid comprising:

A. an absorbent carrier, said absorbent carrier being filter paper; and

B. a reagent in said absorbent carrier, said reagent comprising glucose oxidase, a substance having a peroxidase activity, and an oxidizable indicator; and

C. a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal, the weight ratio of polymethylmethacrylate to polyvinyl formal being 1;

1.

20. A test piece for quantitative analysis of a substance in body fluid, said test piece comprising:

A. an absorbent carrier, wherein said absorbent carrier is a member selected from the group consisting of filter paper, cotton, wood piece, synthetic fiber paper, glass fiber, and porous plastics; and

B. a reagent in said absorbent carrier, said reagent comprising;

1. a substance having a peroxidase activity,

2. an oxidizable indicator, and

3. a member selected from the group consisting of glucose oxidase, pyruvic acid oxidase, galactose oxidase, and alcohol dehydrogenase; and

C. a coating on a surface of the absorbent carrier, said coating comprising polymethylmethacrylate and polyvinyl formal, wherein the weight ratio of polymethylmethacrylate to polyvinyl formal is between 9;

1 and 1;

9.