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Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques

  • US 4,476,228 A
  • Filed: 11/08/1982
  • Issued: 10/09/1984
  • Est. Priority Date: 11/08/1982
  • Status: Expired due to Term
First Claim
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1. A method for determining unsaturated thyroxine binding protein sites in a sample containing thyroxine binding proteins, comprising:

  • (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0;

    (b) contacting the treated sample with a fluorescent labeled tracer wherein said fluorescent labeled tracer has a preferential binding affinity for thyroxine binding globulin, thereby forming a complex between the thyroxine binding proteins and fluorescent labeled tracer; and

    (c) determining the amount of complex formed utilizing fluorescence polarization techniques, as a measure of the unsaturated thyroxine binding protein sites in the sample.

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