Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
First Claim
Patent Images
1. A method for determining unsaturated thyroxine binding protein sites in a sample containing thyroxine binding proteins, comprising:
- (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0;
(b) contacting the treated sample with a fluorescent labeled tracer wherein said fluorescent labeled tracer has a preferential binding affinity for thyroxine binding globulin, thereby forming a complex between the thyroxine binding proteins and fluorescent labeled tracer; and
(c) determining the amount of complex formed utilizing fluorescence polarization techniques, as a measure of the unsaturated thyroxine binding protein sites in the sample.
1 Assignment
0 Petitions
Accused Products
Abstract
This invention encompasses a method for measuring unsaturated thyroxine binding protein sites in a sample comprising intermixing with the sample an effective amount of fluorescent labeled tracer having binding affinity for thyroxine binding proteins and then measuring the amount of tracer bound to the thyroxine binding protein using fluorescence polarization techniques.
-
Citations
10 Claims
-
1. A method for determining unsaturated thyroxine binding protein sites in a sample containing thyroxine binding proteins, comprising:
- (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0;
(b) contacting the treated sample with a fluorescent labeled tracer wherein said fluorescent labeled tracer has a preferential binding affinity for thyroxine binding globulin, thereby forming a complex between the thyroxine binding proteins and fluorescent labeled tracer; and
(c) determining the amount of complex formed utilizing fluorescence polarization techniques, as a measure of the unsaturated thyroxine binding protein sites in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- (a) treating the sample with a composition comprising a nonfluorescent surfactant in a buffered medium having a pH greater than 7.0;
Specification