Fiber optic P.sub.O.sbsb.2 probe

• US 4,476,870 A
• Filed: 07/07/1982
• Issued: 10/16/1984
• Est. Priority Date: 03/30/1982
• Status: Expired due to Term

• Current Assignee: United States Department Of Health And Human Services (Government of the United States of America)
• Original Assignee: United States Department Of Health And Human Services (Government of the United States of America)
• Inventors: Peterson, John I., Fitzgerald, Raphael V.
• Primary Examiner(s): Howell, Kyle L.
• Assistant Examiner(s): Hanley, J. C.
• Application Number:
  US06/396,055
• Time in Patent Office: 832 Days
• Field of Search: 128/633-635, 128/664, 128/665, 128/666, 356/39-41, 250/458.1, 250/459.1
• US Class Current: 600/312
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

A fiber optic probe to be implanted in human body tissue for physiologic studies involving measurement and monitoring of the partial pressure of gaseous oxygen in the blood stream, which is coursing through a particular blood vessel in the body. The use of the probe is based on the principle of dye fluorescence oxygen quenching. Structurally the probe comprises two 150-micrometer strands of plastic optical fiber ending in a section of porous polymer tubing serving as a jacket or envelope for the fibers. The tubing is packed with a suitable fluorescent light-excitable dye placed on a porous adsorptive particulate polymeric support. The tubing or jacket is usually made of a hydrophobic, gas-permeable commercial material, known as Celgard, but other suitable hydrophobic gas-permeable material could be used for such structure. The fiber optic probe of the invention is of very small size and flexible so that it can easily be threaded through small blood vessels which are located in a variety of tissues of the body.

Claims

1. A probe for determining P_O.sbsb.2 in the blood or tissue of a living animal, comprising:

an oxygen-porous jacket of a size sufficiently small to be passed into a blood vessel;

a porous dye support carried within said jacket, said dye support and said jacket having sufficiently high permeability to permit the exposure of individual dye molecules carried thereby to oxygen collision;

a non-toxic luminescent dye carried by said porous dye support, said dye being excitable by visible light, having and sufficiently great stability to aging to permit storage without substantial change of dye characteristics, and being oxygen quenching-sensitive; and

fiber optic means to pass excitation light to said dye within said jacket and collect luminescence therefrom.

2. A probe according to claim 1, wherein said dye is perylene dibutyrate.

3. A probe according to claim 1 or claim 2, wherein said porous dye support is a porous organic polymer.

4. A probe according to claim 1, and wherein said porous dye support comprises silica gel.

5. A probe according to claim 1, and wherein said porous dye support comprises a porous adsorptive particulate polymeric material.

6. A probe according to claim 1, and wherein said porous dye support comprises Amberlite XAD4.

7. A probe according to claim 1, and wherein said oxygen-porous jacket comprises a tubular envelope of porous material.

8. A probe according to claim 7, and wherein said tubular envelope is formed of Celgard.

9. A probe according to claim 1, and wherein said oxygen-porous jacket comprises porous polypropylene sheet material heat-sealed into tubing, closed at one end and provided at said closed end with a tapered closure tip.

10. A probe according to claim 1, and wherein said fiber optic means comprises at least one strand of transparent plastic fiber with one end extending into said jacket and being optically exposed to said dye.

11. A probe according to claim 1, and wherein said fiber optic means comprises two strands of transparent flexible plastic fiber with ends extending into said jacket and being optically exposed to said dye.

12. A probe according to claim 1, and wherein said fiber optic means includes a strand of transparent plastic fiber with one end extending into said jacket and being optically exposed to said dye, optical beam-splitting means optically exposed to the other end of said plastic fiber and forming two spaced optical beams from the light transmitted through the fiber, respective photoelectric signal generating means in the paths of said two optical beams, and P_O.sbsb.2 computing circuit means connected to the outputs of said photoelectric signal generating means.

13. A probe according to claim 12, and respective different-color filter means optically interposed in the paths of the two optical beams between the beam-splitting means and the photoelectric signal generating means.

14. A probe according to claim 13, and wherein one color filter means passes only light corresponding to the luminescence wavelength of the dye, and the other color filter means passes light only of a color corresponding to that of scattered incident light to which the dye is exposed and which is reflected from the dye.