Multichannel spectrophotometer

• US 4,477,190 A
• Filed: 07/20/1981
• Issued: 10/16/1984
• Est. Priority Date: 07/20/1981
• Status: Expired due to Term

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: American Hospital Supply Corp. (Baxter International Inc.)
• Inventors: Stark, William A., Liston, Max D., Dickinson, David G.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US06/284,841
• Time in Patent Office: 1,184 Days
• Field of Search: 356/409, 356/414, 356/418, 356/419, 356/420, 356/433, 356/434, 356/435
• US Class Current: 356/418
• CPC Class Codes: G01N 2021/3174 Filter wheel
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00

Abstract

A multichannel spectrophotometer has a single radiant energy source formed into a multiplicity of radiant energy beams which are each simultaneously intercepted by segments of a rotary source filter wheel, the spectral radiant energy output selected by each filter segment being directed into a separate source radiant energy conduit and transmitted to a remote sample station where a cuvette containing a sample to be analyzed is located, the radiant energy outputs of each cuvette being directed into a separate detector radiant energy conduit where each conduit is intercepted by a filter segment of a second rotating detector filter wheel identical to the source filter wheel and rotated in aligned synchronism therewith, the spectral radiant energy outputs of each segment of the detector filter wheel being further directed into a separate radiant energy detector and signal processing electronics associated with that sample station or spectrophotometer channel from which the radiant energy signal being detected and analyzed has been received. Central control, timing and display electronics are shared by each channel and may be overall programmed and controlled by computer. In one application involving the measurement of fluorescence, the filters of the detector filter wheel will be different from the filters of the source filter wheel so as to excite the sample cuvette at one wavelength and to measure fluorescent response at another.

Claims

1. A spectrophotometer for the simultaneous measurement of the transmission characteristics of a multiplicity of samples at selected spectral wave lengths comprising:

a single radiant energy source;

optical means for forming said single radiant energy source into a circular pattern of a multiplicity of substantially parallel beams of radiant energy;

a source filter wheel, having a multiplicity of spectrally selective filters arranged in a circular pattern substantially matching the circular pattern of said optical means, said source filter wheel and said filters thereon being positioned and arranged such that each filter sequentially receives and selectively transmits spectral portions of each one of the multiplicity of beams as said source filter wheel is rotated;

means for rotating said source filter wheel;

a multiplicity of source radiant energy conduits, one end of each source conduit being oppositely disposed one of said multiplicity of beams of radiant energy such that as said source filter wheel rotates each filter of said source filter wheel passes and intercepts each beam and transmits into each respective source conduit the spectrum associated with that filter for transmission through a sample cuvette;

a multiplicity of sample cuvettes, each one located at the other end of one of said multiplicity of source conduits for holding therein a sample and receiving therethrough the selected spectrum transmitted by said one of said multiplicity of source conduits; and

detection and display means for receiving said selected spectrum transmitted through each of said multiplicity of sample cuvettes and detecting and displaying the transmission effects of the sample in each cuvette on said selected spectrum passed therethrough, said detection and display means being connected to said means for rotating said source filter wheel to synchronize and coordinate said display with the sequence of filter wheel filters.

2. The spectrophotometer described in claim 1 wherein said optical means includes:

a parabolic mirror having said single radiant energy source substantially located at the focal point thereof;

a lens mounting disk located in front of said parabolic mirror adjacent said filter wheel having a multiplicity of lens openings therethrough equally spaced from the axis of said parabolic mirror and circularly arranged therearound; and

a multiplicity of lenses mounted in said multiplicity of lens openings of said lens mounting disk, for collecting radiant energy reflected by said parabolic mirror and directing said radiant energy through said filters of said filter wheel and into said multiplicity of source radiant energy conduits.

3. The spectrophotometer described in claim 2 wherein said lenses are plano-convex.

4. The spectrophotometer described in claim 1 wherein said detector and display means include:

a multiplicity of detector radiant energy conduits each having one end oppositely disposed one of said multiplicity of sample cuvettes to receive therefrom the selected spectrum of electromagnetic energy passed therethrough and to transmit said spectrum to the other end of said each detector conduit;

a detector filter wheel connected to said means for rotating said source filter wheel and having a given arrangement and sequence of filters, said detector radiant energy conduits being arranged such that a given filter of said detector filter wheel receives the spectral energy transmitted by a given filter of said source filter wheel;

a multiplicity of radiant energy detectors for converting the radiant energy falling thereon to electrical signals, each of said multiplicity of radiant energy detectors being located adjacent said detector filter wheel and oppositely disposed one of said multiplicity of detector radiant energy conduits such that as said detector filter wheel rotates the selected spectral radiant energy exiting from each of said multiplicity of detector radiant energy conduits passes through the corresponding filter on said detector filter wheel and into a radiant energy detector; and

electronic means for processing the electrical signals from said multiplicity of radiant energy detectors for analyzing and displaying the transmission effects of each sample cuvette in response to the selected spectra passed therethrough.

5. The spectrophotometer described in claim 4 further including a blank segment in each of the source and detector filter wheels and further including a holding amplifier and means for gating the voltage offset appearing in a particular channel when said filter wheel blank segments appear in that channel to said holding amplifier such that any voltage offset which appears in that channel when the filter blanks are positioned in that channel, is detected, gated and held to be applied as an input to that channel to reduce the voltage offset of that channel to substantially a true zero.

6. The spectrophotometer described in claim 4 further including an automatic gain control circuit comprising:

a gate which is operated to transmit the amplitude of a first few of the train signal pulses in a particular channel to a capacitor;

a capacitor to hold the value of these pulse amplitudes;

an operational amplifier having an input connected to the capacitor and an output coupled to an optical coupler;

an optical coupler connected to a high voltage control;

a high voltage control connected to a high voltage supply; and

a high voltage supply for a photomultiplier detector.

7. The spectrophotometer described in claim 4 wherein in said source and detector filter wheels have the same identical arrangement and sequence of filters such that a given filter of said detector filter wheel receives the spectral energy transmitted by the same identical filter of said source filter wheel.

8. The spectrophotometer described in claim 4 wherein said detector filter wheel is not identical to said source filter wheel and contains filters which are not the same as the filters of said source filter wheel whereby the sample cuvette can be excited by electromagnetic radiation at one wavelength and the fluorescent response thereto measured at another wavelength.

9. A Multichannel Spectrophotometer, comprising:

a single source of radiant energy;

optical means for forming said single source into a multiplicity of beams of radiant energy;

a first filter wheel adjacent said optical means and having a multiplicity of filter segments thereon, each filter segment intercepting one at a time each of said multiplicity of beams of radiant energy as the filter wheel rotates, each of said filter segments defining a band of spectral radiant energy;

means for transmitting each band of spectral radiant energy received from each filter segment to a measurement station;

a multiplicity of measurement stations each having a cuvette containing a sample to be measured by passing the spectral radiant energy received from said first filter wheel therethrough;

means for transmitting the radiant energy output received from each measurement station to a second filter wheel; and

a second filter wheel identical to said first filter wheel each filter segment intercepting one at a time each radiant energy output from said measurement stations, said second filter wheel being rotated in aligned synchronism with said first filter wheel such that a given band of spectral radiant energy transmitted through a given filter of said first filter wheel is received and transmitted through the same filter of said second filter wheel.

10. A multichannel spectrophotometer including optics for forming a multiplicity of radiant energy beams, comprising:

a parabolic reflector;

a source of radiant energy mounted at the focal point of said parabolic reflector;

a series of light baffles having a circular pattern of a multiplicity of circular holes therearound aligned and centered abut the axis of said parabolic reflector;

a lens disk adjacent to said light baffles having a multiplicity of circular lens assemblies aligned with said circular pattern of circular holes in said light baffles, each of said lens assemblies having a lens therein for forming a beam of radiant energy therefrom, said lens disk thereby providing a multiplicity of radiant energy beams therefrom; and

,

a source filter wheel, having a multiplicity of spectrally selective filters arranged in a circular pattern substantially matching the circular pattern of lens within said lens disk, said source filter wheel and said filters thereon being positioned and arranged so that each filter sequentially receives and selectively transmits spectral portions of each one of said beams of radiant energy from said lens assembly as said source filter wheel is rotated.