Quantitative analysis film and a method for colorimetric analysis using the same
First Claim
1. A quantitative analysis film comprising a reagent layer containing a N,N-disubstituted aniline cationic dye-forming color indicator composition for detecting hydrogen peroxide comprising components capable of forming said colored cationic dye by chemical interaction in the presence of hydrogen peroxide, and an anionic polymer capable of reacting with the formed cationic dye to fix the formed dye.
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Abstract
A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.
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18 Claims
- 1. A quantitative analysis film comprising a reagent layer containing a N,N-disubstituted aniline cationic dye-forming color indicator composition for detecting hydrogen peroxide comprising components capable of forming said colored cationic dye by chemical interaction in the presence of hydrogen peroxide, and an anionic polymer capable of reacting with the formed cationic dye to fix the formed dye.
- 13. A method for quantitatively assaying hydrogen peroxide of a precursor capable of forming hydrogen peroxide contained in a test sample colorimetrically which comprises bringing said test sample into contact with a N,N-disubstituted aniline cationic dye-forming color indicator for detecting hydrogen peroxide comprising components capable of forming said colored cationic dye by chemical mutual action in the presence of hydrogen peroxide and then binding the cationic dye formed to an anionic polymer to fix the formed cationic dye.
Specification