Method and apparatus for preparative gel electrophoresis
First Claim
1. A method of forming a gel column for preparative electrophoresis comprisingproviding inner and outer coolable cylinders and a stationary lower end adapter at the bottom thereof forming an annular chamber;
- providing a source of polymerizable gel solution having selectable characteristics;
providing an upper end adapter which is movable between the upper end of the cylinders and a position adjacent the lower end adapter;
conducting polymerizable gel solution from the gel source into the space between the adapters and the cylinders while the upper adapter is concurrently elevated, thereby arranging the gel solution in gradients and building a gel column in the chamber, until the desired column height is reached;
and allowing the gel to polymerize.
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Abstract
A preparative gel electrophoresis apparatus comprises a first outer coolable cylinder, a second inner coolable cylinder within the outer cylinder, a compact column of separating material placed between the first and second cylinders and electrodes arranged above and below the separating column for forming an electrical field over the volume of the separating column, axially translatable adapters above and below the support, surrounding the electrodes, containing duct systems and completely surrounding the inner coolable cylinder. A method of forming the gel column by injecting gel while separating the adapters is also disclosed.
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Citations
22 Claims
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1. A method of forming a gel column for preparative electrophoresis comprising
providing inner and outer coolable cylinders and a stationary lower end adapter at the bottom thereof forming an annular chamber; -
providing a source of polymerizable gel solution having selectable characteristics; providing an upper end adapter which is movable between the upper end of the cylinders and a position adjacent the lower end adapter; conducting polymerizable gel solution from the gel source into the space between the adapters and the cylinders while the upper adapter is concurrently elevated, thereby arranging the gel solution in gradients and building a gel column in the chamber, until the desired column height is reached; and allowing the gel to polymerize. - View Dependent Claims (2, 3, 4)
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5. An apparatus for preparative gel electrophoresis comprising
an outer coolable cylinder; -
an inner coolable cylinder within said outer cylinder; and upper and lower adapters between said inner and outer cylinders, each of said adapters being axially movable and having means forming fluid seals between said adapter and said cylinders, electrode means connectable to a source of electrical energy for forming an electric field between said adapters, and duct means in each of said adapters for conducting fluids into and out of said adapters to comprise an electrode and elution buffer rinsing system, said outer and inner cylinders and said upper and lower adapters defining a chamber for receiving a column of separating material through which said electric field passes. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification